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Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide–Mg2+ interaction

  • Ion Channels, Receptors and Transporters
  • Published:
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Abstract

The effect of nucleotides on single chloride channels derived from rat hepatocyte rough endoplasmic reticulum vesicles incorporated into bilayer lipid membrane was investigated. The single chloride channel currents were measured in 200/50 mmol/l KCl cis/trans solutions. Adding 2.5 mM adenosine triphosphate (ATP) and adenosine diphosphate (ADP) did not influence channel activity. However, MgATP addition inhibited the chloride channels by decreasing the channel open probability (Po) and current amplitude, whereas mixture of Mg2+ and ADP activated the chloride channel by increasing the Po and unitary current amplitude. According to the results, there is a novel regulation mechanism for rough endoplasmic reticulum (RER) Cl− channel activity by intracellular MgATP and mixture of Mg2+ and ADP that would result in significant inhibition by MgATP and activation by mixture of Mg2+ and ADP. These modulatory effects of nucleotide–Mg2+ complexes on chloride channels may be dependent on their chemical structure configuration. It seems that Mg–nucleotide–ion channel interactions are involved to produce a regulatory response for RER chloride channels.

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Abbreviations

HEPES:

4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid N-(2-hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) potassium salt

RER:

Rough endoplasmic reticulum

mitoKATP :

Mitochondrial ATP-sensitive K+ channel

DIDS:

4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid

CFTR:

Cystic fibrosis transmembrane conductance regulator

VDAC:

Voltage-dependent anion channel

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Acknowledgments

This work was supported by a grant from the Cellular and Molecular Research Center of Babol University of Medical Sciences in collaboration with Neuroscience Research Center of Shahid Beheshti Medical Sciences University. We thank Dr. A. Eliassi for her generous guides.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Department of Physiology and Pharmacology, Babol University of Medical Sciences, Babol, Iran

    M. Ashrafpour

  2. Neuroscience Research Center and Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    J. Fahanik Babaei

  3. Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran

    R. Saghiri

  4. Neuroscience Research Center and Department of Physiology, Golestan University of Medical Sciences, Gorgan, Iran

    H. Sepehri

  5. Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

    H. Sharifi

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  1. M. Ashrafpour
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  2. J. Fahanik Babaei
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  3. R. Saghiri
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  4. H. Sepehri
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  5. H. Sharifi
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Correspondence to M. Ashrafpour.

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Ashrafpour, M., Babaei, J.F., Saghiri, R. et al. Modulation of the hepatocyte rough endoplasmic reticulum single chloride channel by nucleotide–Mg2+ interaction. Pflugers Arch - Eur J Physiol 464, 175–182 (2012). https://doi.org/10.1007/s00424-012-1121-z

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  • DOI: https://doi.org/10.1007/s00424-012-1121-z

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