Pflügers Archiv - European Journal of Physiology

, Volume 463, Issue 2, pp 309–318 | Cite as

Differential pathways for calcium influx activated by concanavalin A and CD3 stimulation in Jurkat T cells

  • Bo Pang
  • Dong Hoon Shin
  • Kyung Sun Park
  • Yun Jeong Huh
  • Joohan Woo
  • Yin-Hua Zhang
  • Tong Mook KangEmail author
  • Ki-Young Lee
  • Sung Joon KimEmail author
Ion Channels, Receptors and Transporters


Sustained increase in [Ca2+]c (Δ[Ca2+]c) is a critical early signal from T-cell receptor (TCR/CD3). In general, Ca2+-release activated Ca2+ channels (CRAC) are responsible for the Ca2+ influx and Δ[Ca2+]c after TCR/CD3 stimulation. However, T cells also express Ca2+-permeable nonselective cation channels such as TRPM2 and TRPC. Gd3+ is a relatively selective blocker for CRAC at micromolar concentrations. Here, Jurkat T cells were used to investigate the Gd3+-resistant Ca2+ influx (Δ[Ca2+]c,Gd) induced by concanavalin A (ConA, 1 μg/ml), a widely used mitogenic agent for T cells, or by anti-CD3 Ab (αCD3). αCD3-induced Δ[Ca2+]c was partly (~60%) inhibited by 1 μM Gd3+ while thapsigargin-induced Δ[Ca2+] was almost completely abolished. ConA-induced Δ[Ca2+] was mostly inhibited by 1 μM Gd3+ during the early phase (<30 s of ConA application) and became resistant during the late phase (>2 min). Induction of Δ[Ca2+]c,Gd by αCD3 and ConA was inhibited by 2-aminoethoxydiphenyl borate (2-APB) and by N-(p-amylcinnamoyl) anthranilic acid, indicating that TRPM2 and TRPC are involved in this process. Treatment with Pyr-3, a TRPC3-specific inhibitor, potently suppressed Δ[Ca2+]c,Gd by αCD3 (IC50, 0.16 μM). Patch clamp experiments demonstrated that the TRPM2 channels were activated by ConA, and the TRPC-like channels were activated by αCD3. Our present study suggests that TRPM2 and TRPC3 are activated by ConA and TCR/CD3, respectively, in Jurkat T cells and are responsible for the induction of Δ[Ca2+]c,Gd.


Ca2+ influx Calcium signaling Lymphocyte Nonselective cation channel TRP channels 



This work was supported by a Korea Research Foundation Grant funded by the Korean Government (KRF-2008-314-E00008).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Bo Pang
    • 1
    • 2
  • Dong Hoon Shin
    • 1
  • Kyung Sun Park
    • 3
  • Yun Jeong Huh
    • 1
  • Joohan Woo
    • 1
  • Yin-Hua Zhang
    • 1
  • Tong Mook Kang
    • 4
    Email author
  • Ki-Young Lee
    • 5
  • Sung Joon Kim
    • 1
    • 6
    Email author
  1. 1.Department of PhysiologySeoul National University College of MedicineSeoulRepublic of Korea
  2. 2.Key Laboratory of Hormones and Development, Ministry of Health Metabolic Diseases HospitalTianjin Medical UniversityTianjinChina
  3. 3.Division of Integrative Biosciences and BiotechnologyPohang University of Science and TechnologyPohangRepublic of Korea
  4. 4.Department of PhysiologySBRI, Sungkyunkwan University School of MedicineSuwonRepublic of Korea
  5. 5.Department of Molecular Cell BiologySBRI, Sungkyunkwan University School of MedicineSuwonRepublic of Korea
  6. 6.Ischemic/Hypoxic Disease InstituteSeoul National University College of MedicineSeoulRepublic of Korea

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