Fig. 3
From: Presynaptically expressed long-term depression at cerebellar parallel fiber synapses
Long-term depression of parallel fiber presynaptic Ca2+ transients induced by 4 Hz parallel fiber stimulation. a 1–3 4 Hz PF stimulation in the presence of H-89 induced a long-term depression of PF Ca2+ transients. 1 Left, fluorescent image of a coronal cerebellar slice from a GCaMP2 transgenic mouse with stimulation electrode (S) placed in the molecular layer. Yellow rectangle indicates cropped region used for color-coded maps of peak Ca2+ transients in response to a single PF stimulation pulse, before (Pre1–Pre3) and after (Post1–Post4) LTD induction (arrowhead). 2 Time course of PF Ca2+ transients obtained from responsive area (red outline in 1). 3 Time course of the baseline-normalized peak amplitude of Ca2+ transient from the experiment illustrated in 1 and 2. b Time course of the baseline-normalized mean peak amplitude of PF Ca2+ transient before and after delivery of 120 pulses to the PFs at 1 Hz (n = 7), 2 Hz (n = 7), 4 Hz (n = 8), and 8 Hz (n = 8). Note that LTD was induced with 4–8 Hz PF stimulation but not with 1–2 Hz stimulation. c Mean baseline-normalized PF Ca2+ transient 40 min after PF stimulation at 1–8 Hz. All experiments were conducted in the presence of 10 μM H-89. Data points in b and c are presented as the mean±SEM