Abstract
We conducted non-invasive methods to investigate the mechanisms how an orthostasis improves fatigue resistance in human calf muscle during intense exercise. Eleven healthy volunteers performed two series of ten intervals of maximum dynamic exercise (15 s) and recovery (45 s) at almost horizontal body position under both, control conditions (CON) and lower body negative pressure (LBNP, −40 mbar). As from the second work interval, LBNP significantly improved fatigue resistance shown as a lower reduction in work and in contraction velocity (P < 0.01). During each work interval, EMG showed a small increase in amplitude (P < 0.01) and a steep drop by 20% in median frequency (P < 0.01). Under LBNP, both EMG parameters completely recovered during subsequent rest, whereas under CON recovery was incomplete (P < 0.01). During the first work interval, consumption of phosphocreatine (PCr) was almost the same for both conditions. In periods of recovery under LBNP, resynthesis of PCr and inorganic phosphate were significantly faster. PCr reached 10 to 20% higher levels (P < 0.01). LBNP caused an initial increase in intracellular pH (0.08 U (P < 0.01)). The subsequent time courses of pH were similar for CON and LBNP. During work, pH steeply increased by about 0.3 U. During subsequent recovery, pH dropped to values between 6.3 and 6.5. LBNP caused significantly higher levels of total haemoglobin and oxy-haemoglobin (P < 0.05). A simulated orthostasis increased fatigue resistance during high intense interval exercise because of a faster PCr resynthesis and may be because of improvements in the maintenance of motoneuronal activity.
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Arnold DL, Matthews PM, Radda GK (1984) Metabolic recovery after exercise and the assessment of mitochondrial function in vivo in human skeletal muscle by means of 31P NMR. Magn Reson Med 1:307–315
Baerwalde S, Zange J, Müller K, Maassen N (1999) High-energy-phosphates measured by 31P-MRS during LBNP in exercising human leg muscle. J Grav Physiol 6:37–38
Binzoni T, Quaresima V, Ferrari M, Hiltbrand E, Cerretelli P (2000) Human calf microvascular compliance measured by near-infrared spectroscopy. J Appl Physiol 88:369–372
Caffier G, Rehfeldt H, Kramer H, Mucke R (1992) Fatigue during sustained maximal voluntary contraction of different muscles in humans: dependence on fibre type and body posture. Eur J Appl Physiol 64:237–243
Chance B, Leigh JS, Kent J, McCully K (1986) Metabolic control principles and 31P NMR. Fed Proc 45:2915–2920
Chance B, Leigh JSJ, Kent J, McCully K, Nioka S, Clark BJ, Maris JM, Graham T (1986) Multiple controls of oxidative metabolism in living tissues as studied by phosphorus magnetic resonance. Proc Natl Acad Sci USA 83:9458–9462
Christ M, Zange J, Janson CP, Müller K, Kuklinski P, Schmidt BM, Tillmann HC, Gerzer R, Wehling M (2001) Hypoxia modulates rapid effects of aldosterone on oxidative metabolism in human calf muscle. J Endocrinol Invest 24:587–597
Crowther GJ, Carey MF, Kemper WF, Conley KE (2002) Control of glycolysis in contracting skeletal muscle. I. Turning it on. Am J Physiol 282:E67–E73
De Blasi RA, Ferrari M, Natali A, Conti G, Mega A, Gasparetto A (1994) Noninvasive measurement of forearm blood flow and oxygen consumption by near-infrared spectroscopy. J Appl Physiol 76:1388–1393
Duncan A, Meek JH, Clemence M, Elwell CE, Tyszczuk L, Cope M, Delpy DT (1995) Optical pathlength measurements on adult head, calf and forearm and the head of the newborn infant using phase resolved optical spectroscopy. Phys Med Biol 40:295–304
Egaña M, Green S (2005) Effect of body tilt on calf muscle performance and blood flow in humans. J Appl Physiol 98:2249–2258
Eiken O (1988) Effects of increased muscle perfusion pressure on responses to dynamic leg exercise in man. Eur J Appl Physiol 57:772–776
Gasiorowska A, Nazar K, Mikulski T, Cybulski G, Niewiadomski W, Smorawinski J, Krzeminski K, Porta S, Kaciuba-Uscilko H (2005) Hemodynamic and neuroendocrine predictors of lower body negative pressure (LBNP) intolerance in healthy young men. J Physiol Pharmacol 56:179–193
Hamaoka T, Katsumura T, Murase N, Sako T, Higuchi H, Murakami M, Esaki K, Kime R, Homma T, Sugeta A, Kurosawa Y, Shimomitsu T, Chance B (2003) Muscle oxygen consumption at onset of exercise by near infrared spectroscopy in humans. Adv Exp Med Biol 530:475–483
Hinghofer-Szalkay HG, Vigas M, Sauseng-Fellegger G, König EM, Lichardus B, Jezova D (1996) Head-up tilt and lower body suction: comparison of hormone responses in healthy men. Physiol Res 45:369–378
Iotti S, Gottardi G, Clementi V, Barbiroli B (2004) The mono-exponential pattern of phosphocreatine recovery after muscle exercise is a particular case of a more complex behaviour. Biochim Biophys Acta 1608:131–139
Kahn JF, Huart F, Kapitaniak B, Monod H (1986) Effect of arm position on cardiovascular responses during isometric handgrips. Eur J Appl Physiol Occup Physiol 55:88–92
Kemp GJ, Taylor DJ, Radda GK (1993) Control of phosphocreatine resynthesis during recovery from exercise in human skeletal muscle. NMR Biomed 6:66–72
Kemp GJ, Taylor DJ, Thompson CH, Hands LJ, Rajagopalan B, Styles P, Radda GK (1993) Quantitative analysis by 31P magnetic resonance spectroscopy of abnormal mitochondrial oxidation in skeletal muscle during recovery from exercise. NMR Biomed 6:302–310
Kushmerick MJ, Meyer RA, Brown TR (1992) Regulation of oxygen consumption in fast- and slow-twitch muscle. Am J Physiol 263:C598–C606
Lösel R, Schultz A, Boldyreff B, Wehling M (2004) Rapid effects of aldosterone on vascular cells: clinical implications. Steroids 69:575–578
Marcinek DJ, Ciesielski WA, Conley KE, Schenkman KA (2003) Oxygen regulation and limitation to cellular respiration in mouse skeletal muscle in vivo. Am J Physiol Heart Circ Physiol 285:H1900–H1908
Molé PA, Chung Y, Tran TK, Sailasuta N, Hurd R, Jue T (1999) Myoglobin desaturation with exercise intensity in human gastrocnemius muscle. Am J Physiol 277:R173–R180
Rothman DL, Shulman RG, Shulman GI (1992) 31P nuclear magnetic resonance measurements of muscle glucose-6- phosphate. Evidence for reduced insulin-dependent muscle glucose transport or phosphorylation activity in non-insulin-dependent diabetes mellitus. J Clin Invest 89:1069–1075
Sahlin K, Edström L, Sjöholm H (1983) Fatigue and phosphocreatine depletion during carbon dioxide-induced acidosis in rat muscle. Am J Physiol 245:C15–C20
Saunders NR, Dinenno FA, Pyke KE, Rogers AM, Tschakovsky ME (2005) Impact of combined NO and PG blockade on rapid vasodilation in a forearm mild-to-moderate exercise transition in humans. Am J Physiol Heart Circ Physiol 288:H214–H220
Saunders NR, Pyke KE, Tschakovsky ME (2005) Dynamic response characteristics of local muscle blood flow regulatory mechanisms in human forearm exercise. J Appl Physiol 98:1286–1296
Saunders NR, Tschakovsky ME (2004) Evidence for a rapid vasodilatory contribution to immediate hyperemia in rest-to-mild and mild-to-moderate forearm exercise transitions in humans. J Appl Physiol 97:1143–1151
Schneider G, Koch H, Maassen N, Leibfritz D (1999) 31P NMR Spectroscopy of the human calf Muscle during intensive interval exercise. Eur J Appl Physiol 69:S14
Shushakov V, Stubbe C, Peuckert A, Endeward V, Maassen N (2007) The relationships between plasma potassium, muscle excitability, and fatigue during voluntary exercise. Exp Physiol 92:705–715
Søgaard K, Gandevia SC, Todd G, Petersen NT, Taylor JL (2006) The effect of sustained low-intensity contractions on supraspinal fatigue in human elbow flexor muscles. J Physiol 573:511–523
Taylor DJ, Bore PJ, Styles P, Gadian DG, Radda GK (1983) Bioenergetics of intact human muscle. A 31P nuclear magnetic resonance study. Mol Biol Med 1:77–94
Tran TK, Sailasuta N, Kreutzer U, Hurd R, Chung Y, Mole P, Kuno S, Jue T (1999) Comparative analysis of NMR and NIRS measurements of intracellular PO2 in human skeletal muscle. Am J Physiol 276:R1682–R1690
Van Beekvelt MC, Colier WN, Wevers RA, Van Engelen BG (2001) Performance of near-infrared spectroscopy in measuring local O(2) consumption and blood flow in skeletal muscle. J Appl Physiol 90:511–519
Vestergaard-Poulsen P, Thomsen C, Sinkjaer T, Henriksen O (1995) Simultaneous 31P-NMR spectroscopy and EMG in exercising and recovering human skeletal muscle: a correlation study. J Appl Physiol 79:1469–1478
Westerblad H, Allen DG, Lännergren J (2002) Muscle fatigue: lactic acid or inorganic phosphate the major cause? News Physiol Sci 17:17–21
Zange J, Müller K, Gerzer R, Sippel K, Wehling M (1996) Nongenomic effects of aldosterone on phosphocreatine levels in human calf muscle during recovery from exercise. J Clin Endocrinol Metab 81:4296–4300
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Zange, J., Beisteiner, M., Müller, K. et al. Energy metabolism in intensively exercising calf muscle under a simulated orthostasis. Pflugers Arch - Eur J Physiol 455, 1153–1163 (2008). https://doi.org/10.1007/s00424-007-0361-9
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DOI: https://doi.org/10.1007/s00424-007-0361-9