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Reduction in β-myosin heavy chains in stunned myocardium as assessed by nondenaturing gel electrophoresis

  • Cardiovascular System
  • Published:
Pflügers Archiv - European Journal of Physiology Aims and scope Submit manuscript

Abstract

Myosin plays a key role in the structure and function of cardiac muscle. Three myosin isoenzymes (V1, V2, and V3) with different ATPase activities have been identified in mammalian ventricles based on their heavy chain constituents. The relative amount of myosin isoenzymes changes under physiological and pathological conditions. Until now, myosin isoenzymes have frequently been determined using either tube gel (nondenaturing) polyacrylamide gel electrophoresis (PAGE), or gradient or uniform sodium dodecyl sulfate (denaturing) PAGE. Both methods have disadvantages, e.g., a long running time. We developed, therefore, a uniform, nondenaturing PAGE with slab minigel format for analyzing the myosin isoenzymes in normoxic and stunned rabbit hearts. In normoxic hearts of adult rabbits, V3 predominated over V1 (46 vs 41%). In turn, in the stunned hearts, V1 predominated over V3 (70 vs 30%), and the heterodimeric V2 was not anymore detectable. This alteration appears to result from a selective loss of myosin heavy chain (MHC)-β. In parallel, the biochemical markers troponin I and creatine kinase were increased in the stunned hearts. We suggest that alterations of myosin isoenzymes in stunned myocardium can be monitored with native PAGE. The present analysis of myosin isoenzyme appears thus as a new tool for evaluating defects in MHC dimer formation in postischemic hearts.

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Acknowledgement

Reading and correcting of the manuscript by Dr. C. Heke is greatly appreciated. Mrs. S.C. Garcia was supported by a fellowship from the German Academic Exchange Service (DAAD) and had a lay-off of the Federal University of Santa Maria/Brazil. The study was supported in part by a grant from the German Research Foundation (DFG; Schi 201/13-1).

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Authors and Affiliations

  1. Research Group Experimental Surgery, Department of Surgery I, University Hospital Duesseldorf, Heinrich-Heine-University, Moorenstraße 5, 40225, Duesseldorf, Germany

    S. C. Garcia, V. J. Pomblum & J. D. Schipke

  2. Clinic of Thoracic and Cardiovascular Surgery, Department of Surgery I, University Hospital Duesseldorf, Heinrich-Heine-University, Duesseldorf, Germany

    E. Gams

  3. Department of Internal Medicine and Cardiology, Molecular Cardiology Laboratory, Philipps University, Marburg, Germany

    H. Rupp

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  1. S. C. Garcia
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  2. V. J. Pomblum
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  3. E. Gams
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  4. H. Rupp
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  5. J. D. Schipke
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Correspondence to S. C. Garcia.

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Garcia, S.C., Pomblum, V.J., Gams, E. et al. Reduction in β-myosin heavy chains in stunned myocardium as assessed by nondenaturing gel electrophoresis. Pflugers Arch - Eur J Physiol 454, 937–943 (2007). https://doi.org/10.1007/s00424-007-0268-5

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  • DOI: https://doi.org/10.1007/s00424-007-0268-5

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