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Pflügers Archiv

, Volume 452, Issue 4, pp 471–477 | Cite as

Renal microvascular constriction to membrane depolarization and other stimuli: pivotal role for rho-kinase

  • Marjon H. Roos
  • William F. van Rodijnen
  • Anton A. van Lambalgen
  • Piet M. ter Wee
  • Geert Jan TangelderEmail author
Smooth Muscle

Abstract

Contraction of vascular smooth muscle is determined not only by levels of intracellular free calcium but also by the sensitivity of its contractile apparatus. A potential modulator of the latter is rho-kinase. We addressed the question of a possible central role for rho-kinase in the regulation of periglomerular microvascular tone. In the rat hydronephrotic kidney model, diameter changes of distal interlobular arteries, afferent and efferent arterioles were measured using three distinctly different stimuli: intravascular pressure changes, angiotensin II (AngII) and membrane depolarization, which is a physiological component of many signaling pathways, as evoked in two ways. Two selective, structurally different rho-kinase inhibitors, Y-27632 and HA-1077, were employed, as well as a selective protein kinase C alpha inhibitor. Preglomerular vasoconstriction induced by direct membrane depolarization, increases in pressure or AngII all depended for their effect on rho-kinase. A differing role for rho-kinase in efferent arteriolar constriction to AngII as compared to preglomerular microvessels was not found. In conclusion, our data indicate that in the kidney, rho-kinase is involved in a variety of signaling pathways leading to microvascular constriction. It plays a pivotal role not only in preglomerular but also in postglomerular tone.

Keywords

Kidney Microvascular tone Rho-kinase Barium chloride Potassium chloride Angiotensin II Myogenic response 

Notes

Acknowledgements

This study was supported by a grant of the Dutch Kidney Foundation, C99.1808. The authors would like to thank Wim Gerrissen for taking care of the animals. A portion of this work was presented at the Experimental Biology 2003, San Diego, CA, and has been published in abstract form in FASEB J 17:A53 (2003).

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Marjon H. Roos
    • 1
  • William F. van Rodijnen
    • 1
  • Anton A. van Lambalgen
    • 1
  • Piet M. ter Wee
    • 2
  • Geert Jan Tangelder
    • 1
    Email author
  1. 1.Laboratory for Physiology, Institute for Cardiovascular ResearchVU University Medical CenterAmsterdamThe Netherlands
  2. 2.Department of Nephrology, Institute for Cardiovascular ResearchVU University Medical CenterAmsterdamThe Netherlands

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