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The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons

  • Cellular Neurophysiology
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Abstract

Neuronal depolarization causes larger intracellular pH (pHi) shifts in axonal and dendritic regions than in the cell body. In this paper, we present evidence relating the time for collapse of these gradients to neuronal morphology. We have used ratiometric pHi measurements using 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) in whole-cell patch-clamped snail neurons to study the collapse of longitudinal pH gradients. Using depolarization to open voltage-gated proton channels, we produced alkaline pHi microdomains. In the absence of added mobile buffers, facilitated H+ diffusion down the length of the axon plays a critical role in determining pHi microdomain lifetime, with axons of ∼100 μm allowing pH differences to be maintained for >60 s. An application of mobile, membrane-permeant pH buffers accelerated the collapse of the alkaline-pH gradients but, even at 30 mM, was unable to abolish them. Modeling of the pHi dynamics showed that both the relatively weak effect of the weak acid/base on the peak size of the pH gradient and the accelerated collapse of the pH gradient could be due to the time taken for equilibration of the weak acid and base across the cell. We propose that appropriate weak acid/base mixes may provide a simple method for studying the role of local pHi signals without perturbing steady-state pHi. Furthermore, an extrapolation of our in vitro data to longer and thinner neuronal structures found in the mammalian nervous system suggests that dendritic and axonal pHi are likely to be dominated by local pHi-regulating mechanisms rather than simply following the soma pHi.

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Acknowledgements

AP was supported by a Vacation Studentship from the Wellcome Trust. We thank the MRC for past support, and acknowledge its desire to have funded this work. We also acknowledge the University of Cambridge for consumables funding and Roger Thomas for comments on the manuscript.

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Authors and Affiliations

  1. Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK

    A. Pantazis & M. Postma

  2. Department of Physiology, University of Cambridge, Downing Street, Cambridge, CB2 3EG, UK

    P. Keegan & C. J. Schwiening

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  1. A. Pantazis
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  2. P. Keegan
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  3. M. Postma
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  4. C. J. Schwiening
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Correspondence to C. J. Schwiening.

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Pantazis, A., Keegan, P., Postma, M. et al. The effect of neuronal morphology and membrane-permeant weak acid and base on the dissipation of depolarization-induced pH gradients in snail neurons. Pflugers Arch - Eur J Physiol 452, 175–187 (2006). https://doi.org/10.1007/s00424-005-0019-4

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  • DOI: https://doi.org/10.1007/s00424-005-0019-4

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