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Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice

  • Renal Function, Body Fluids
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Abstract

Based on the controversy about the relevance of cyclooxygenase-2 (Cox-2)-derived prostanoids from the macula densa for the control of the renin system, this study aimed to determine the interrelation between Cox-2 and renin expression in the mouse kidney. In control mice renin mRNA was readily detectable whilst renocortical Cox-2 mRNA abundance was at the detection limit of the RNase protection assay and no specific signals for Cox-2 were obtained by in situ hybridization or Western blot analysis. Experimental maneuvers such as low-salt diet, treatment with loop diuretics or angiotensin I converting enzyme inhibitors clearly increased renin mRNA abundance up to sevenfold, but under none of these conditions renocortical Cox-2 mRNA levels were significantly changed. Moreover, the strong stimulation of renin expression by angiotensin I-converting enzyme inhibition was not changed by the cyclooxygenase inhibitor ibuprofen, which in turn clearly lowered tissue prostanoid content. Our data suggest a marked divergence of renin and Cox-2 expression in the kidney cortex of C57Bl/6 mice with no clear evidence for a role of Cox-2-derived prostanoids from the macula densa in the regulation of renin expression.

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Acknowledgements

The skillful assistance provided by Marie-Luise Schweiger is gratefully acknowledged. The work was financially supported by grants from the Deutsche Forschungsgemeinschaft (Ku859/12-2).

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Authors and Affiliations

  1. Department of Physiology, University of Regensburg, 93040, Regensburg, Germany

    Charlotte Wagner, Helga Vitzthum, Hayo Castrop, Sybille Albertin & Armin Kurtz

  2. Department of Anatomy, University of Regensburg, 93040, Regensburg, Germany

    Karl Schumacher

  3. Department of Anesthesiology, University of Regensburg, 93040, Regensburg, Germany

    Michael Bucher

  4. Department of Medicine, Duke University, Durham, NC, USA

    Thomas M. Coffman

  5. Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    William J. Arendshorst

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  1. Charlotte Wagner
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Correspondence to Charlotte Wagner.

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Wagner, C., Vitzthum, H., Castrop, H. et al. Differential regulation of renin and Cox-2 expression in the renal cortex of C57Bl/6 mice. Pflugers Arch - Eur J Physiol 447, 214–222 (2003). https://doi.org/10.1007/s00424-003-1157-1

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