Pflügers Archiv

, Volume 445, Issue 6, pp 693–696 | Cite as

The serine/threonine kinases SGK2 and SGK3 are potent stimulators of the epithelial Na+ channel α,β,γ-ENaC

  • B. Friedrich
  • Y. Feng
  • P. Cohen
  • T. Risler
  • A. Vandewalle
  • S. Bröer
  • J. Wang
  • D. Pearce
  • F. Lang
Ion Channels, Transporters


The serum- and glucocorticoid-inducible kinase 1 (SGK1) has been identified as a signalling molecule up-regulated by aldosterone, which stimulates the renal epithelial Na+ channel ENaC. It is therefore thought to participate in the antinatriuretic action of this hormone. More recently, two isoforms, SGK2 and SGK3, have been cloned. The present study was performed to establish whether SGK2 and SGK3 influence ENaC activity similarly to SGK1. Dual-electrode voltage-clamp experiments in Xenopus laevis oocytes expressing α,ß,γ-ENaC with or without SGK1, SGK2 or SGK3 revealed a stimulatory effect of all three kinases on the amiloride-sensitive current (I Na). To establish whether the SGK isoforms exert their effects through direct phosphorylation, we replaced the serine at the SGK consensus site of αENaC (αS622AENaC) by site-directed mutagenesis. αS622A,β,γ-ENaC was up-regulated similar to wild-type ENaC, suggesting that SGK isoforms do not act via direct phosphorylation of the transport proteins. In conclusion, SGK2 and SGK3 mimic the function of SGK1 and are likely to participate in the regulation of ENaC activity.


Sodium channel Sodium reabsorption ENaC Protein kinases Kidney 



The authors are indebted to Dr. B. Rossier (Lausanne, Switzerland) for providing the ENaC constructs. They acknowledge the technical assistance of B. Noll and the meticulous preparation of the manuscript by Leijla Subasic. This study was supported by the Deutsche Forschungsgemeinschaft, Nr. La 315/4-4 and La 315/5-1, the Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (Centre for Interdisciplinary Clinical Research) 01 KS 9602 (to FL) and the UK Medical Research Council and The Royal Society (to PC).


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Copyright information

© Springer-Verlag  2003

Authors and Affiliations

  • B. Friedrich
    • 1
  • Y. Feng
    • 1
  • P. Cohen
    • 2
  • T. Risler
    • 3
  • A. Vandewalle
    • 4
  • S. Bröer
    • 5
  • J. Wang
    • 6
  • D. Pearce
    • 6
  • F. Lang
    • 1
  1. 1.Physiologisches Institut der Universität TübingenTübingenGermany
  2. 2.MRC Protein Phosphorylation Unit, School of Life SciencesUniversity of DundeeGreat Britain
  3. 3.Department of Internal Medicine IIIUniversity of TübingenGermany
  4. 4.INSERM U478 Faculty of Medicine, Xavier BichatParisFrance
  5. 5.Div. of Biochemistry and Molecular BiologyAustralian National UniversityCanberraAustralia
  6. 6.Department of MedicineUniversity of CaliforniaUSA

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