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Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer

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Abstract

Purpose

We previously demonstrated that hepatic ischemia and reperfusion (I/R) injury increased liver metastasis and cancer growth of RCN-H4 cells. Using a rat model of hepatic I/R-induced liver metastasis, we investigated the metastasis-suppressing effect of polysaccharide-K (PSK), a biological response modifier composed of protein-bound polysaccharide.

Methods

Fischer rats underwent 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. PSK was administered orally before I/R, after I/R, or before and after I/R. The weights of metastatic lesions of the liver or the numbers of liver metastatic nodules were determined on day 21. The effect of PSK on angiogenesis was studied by a rat cornea model using RCN-H4 cells or a vascular endothelial growth factor (VEGF)-containing pellet and an in vitro VEGF-induced endothelial cell migration assay.

Results

PSK administration significantly (p < 0.05) suppressed the I/R-induced increase in hepatic metastasis of RCN-H4 cells. The suppression of I/R-promoted metastasis was observed irrespective of the timing of administration. Furthermore, PSK significantly suppressed angiogenesis induced by RCN-H4 cells (p < 0.05) and the VEGF pellet (p < 0.01). PSK significantly suppressed the VEGF-induced migration of vascular endothelial cells (p < 0.05).

Conclusion

PSK may suppress metastasis induced by hepatic I/R. The suppression of angiogenesis by PSK may be one of the mechanisms of the inhibition of hepatic metastasis.

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Conflicts of interest

KT and TH have no potential conflicts of interest. TW, KB, and TA are employees of Kureha Corporation, but the study was conducted with scientific integrity and presents no conflict of interest.

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Authors and Affiliations

  1. Department of Surgery, National Hospital Organization Osaka-Minami Medical Center, 2-1 Kidohigashi-cho, Kawachinagano, Osaka, 586-8521, Japan

    Koji Tamagawa & Tetsuya Horiuchi

  2. Biomedical Research Laboratories, Kureha Corporation, 3-26-2, Hyakunin-cho, Shinjuku, Tokyo, Japan

    Tsutomu Wada, Kenji Bannai & Takao Ando

Authors
  1. Koji Tamagawa
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  2. Tetsuya Horiuchi
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  3. Tsutomu Wada
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  4. Kenji Bannai
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  5. Takao Ando
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Corresponding author

Correspondence to Tetsuya Horiuchi.

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Tamagawa, K., Horiuchi, T., Wada, T. et al. Polysaccharide-K (PSK) may suppress surgical stress-induced metastasis in rat colon cancer. Langenbecks Arch Surg 397, 475–480 (2012). https://doi.org/10.1007/s00423-011-0896-2

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  • DOI: https://doi.org/10.1007/s00423-011-0896-2

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