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Flow distribution during infusion of UW and HTK solution in anaesthetised rats

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Abstract

Purpose

Organ transplantation necessitates the use of preservation solutions to maintain the integrity of the organs during retrieval. The aim of this study was to investigate the flow distribution in abdominal organs in rats during acute infusion of preservation solution.

Methods

Microspheres were used to estimate the distribution of flow in the pancreas, duodenum, ileum, colon, liver, kidneys and lungs in untreated Wistar–Furth rats and in animals with an opened abdominal cavity and catheterised aorta. Some animals were infused by free flow of 5 ml of UW, HTK or Ringer solution containing microspheres at a pressure of 100 cm H2O through an intra-aortic catheter.

Results

Opening of the abdominal cavity did not affect any of the organ blood flow values. However, the fraction of total pancreatic blood flow diverted through the islets increased. During infusion of microsphere-containing UW, HTK or Ringer solution, splanchnic and renal organ flow values, represented by microsphere contents, were similar. The fraction of microspheres found in the islets was lower in UW-infused rats. The number of microspheres present in the lungs or liver was very low, suggesting that shunting was negligible.

Conclusions

Infusion of HTK and UW solution into anaesthetised rats results in a flow distribution which is similar to that in normal animals in most abdominal organs, but there is a reduction in islet blood perfusion by UW but not HTK solution.

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Acknowledgements

The skilled technical assistance of Astrid Nordin is gratefully acknowledged. Financial support was received from the Juvenile Diabetes Research Foundation, the Swedish Research Council (72X-109, 72XD-15043), the Swedish Diabetes Association, Barndiabetesfonden, the NOVO Nordic Research Fund and the Family Ernfors Fund.

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Box 571, 751 23, Uppsala, Sweden

    Leif Jansson, Per-Ola Carlsson, Birgitta Bodin & Örjan Källskog

  2. Department of Medical Sciences, Uppsala University, Uppsala University Hospital, Uppsala, Sweden

    Per-Ola Carlsson

Authors
  1. Leif Jansson
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  2. Per-Ola Carlsson
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  3. Birgitta Bodin
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  4. Örjan Källskog
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Corresponding author

Correspondence to Leif Jansson.

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Author contributions

Leif Jansson: study conception and design; acquisition of data; analysis and interpretation of data; drafting of the manuscript

Per-Ola Carlsson: analysis and interpretation of data; critical revision of the manuscript

Birgitta Bodin: acquisition of data

Örjan Källskog: study conception and design; analysis and interpretation of data; critical revision of the manuscript

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Jansson, L., Carlsson, PO., Bodin, B. et al. Flow distribution during infusion of UW and HTK solution in anaesthetised rats. Langenbecks Arch Surg 396, 677–683 (2011). https://doi.org/10.1007/s00423-011-0747-1

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  • DOI: https://doi.org/10.1007/s00423-011-0747-1

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