Skip to main content
SpringerLink
Log in

p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum

  • Original Article
  • Published:
Langenbeck's Archives of Surgery Aims and scope Submit manuscript

Abstract

Backgrounds and aims

Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method has some important adverse effects on intra-abdominal organs. The aim of this study is to evaluate the effects of different pressures of CO2 on apoptosis and p53 expression in cells in liver and spleen.

Methods

In total, 30 male Sprague–Dawley rats were used in the study. CO2 was insufflated into the intra-abdominal cavity via angiocatheter cannule by an insufflator in two different pressures of 10 and 20 mm Hg for 60 min. However, in the control group, only cannule was inserted into the intra-abdominal cavity, but no gas was insufflated. After 60 min, the rats were killed and laparotomy was applied. The liver and spleen were excised. The samples were histologically processed and immunohistochemistry was applied.

Results

All the data revealed that the number of apoptotic cells in liver and spleen increases in proportion to CO2 pressure level. No p53 expression was detected in both organs.

Conclusion

CO2 pressure level and application time may affect on cells living in liver and spleen. High pressure and/or long application time may cause releasing of cytokines and superoxide radicals from these organs’ cells, and transient or serious organ dysfunctions may occur.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  1. Davis CJ, Filipi CJ (1995) A history of endoscopic surgery. In: Arregui ME, Fitzgibbons RJ, Kotkhoude M, McKernan JB, Reich H (eds) Principles of laparoscopic surgery: basic and advanced techniques. Springer, New York, p 3

    Google Scholar 

  2. Rosario MTA, Ribeiro U Jr, Corbet CEP, Ozaki AC, Bresciani CC, Zilberstein B (2006) Does CO2 pneumoperitoneum alter the ultra-structure of the mesothelium? J Surg Res 133:84–88

    Article  PubMed  Google Scholar 

  3. Lindström P, Kallskog Ö, Wadström J, Persson AEG (2003) Blood flow distribution during elevated intraperitoneal pressure in the rat. Acta Physiol Scand 177:149–156

    Article  PubMed  Google Scholar 

  4. Erikoğlu M, Yol S, Avunduk MC, Erdemli E, Can A (2005) Electron microscopic alterations of the peritoneum after both cold and heated carbon dioxide pneumoperitoneum. J Surg Res 125:73–77

    Article  PubMed  Google Scholar 

  5. Sare M, Yılmaz I, Hamamcı D, Birincioğlu M, Özmen M, Yeşilada Ö (2000) The effect of carbon dioxide pneumoperitoneum on free radicals. Surg Endosc 14:649–652

    Article  PubMed  CAS  Google Scholar 

  6. Kopernik G, Avinoach E, Grossman Y, Levy R, Yulzari R, Rogachev B (1998) The effect of high partial pressure of carbon dioxide environment on metabolism and immune function of human peritoneal cells—relevance to carbon dioxide pneumoperitoneum. Am J Obstet Gynecol 179(6,1):1503–1509

    Article  PubMed  CAS  Google Scholar 

  7. Unsal MA, İmamoğlu M, Kadıoğlu M, Aydın S, Ulku C, Kesim M (2006) The acute alterations in biochemistry, morphology and contractility of rat-isolatd terminal ileum via increased intra-abdominal pressure. Pharm Res 53:135–141

    Article  CAS  Google Scholar 

  8. Gutt CN, Kim ZG, Schmandra T, Paolucci V, Lorenz M (2000) Carbon dioxide pneumoperitoneum is associated with increased liver metastases in a rat model. Surgery 127(5):566–570

    Article  PubMed  CAS  Google Scholar 

  9. Schachtrupp A, Toens Ch, Hoer J, Klosterhalfen B, Lawong AG, Schumpelick VA (2002) 24 h pneumoperitoneum leads to multiple organ impairment in a porcine model. J Surg Res 106:37–45

    Article  PubMed  CAS  Google Scholar 

  10. Levine AJ (1997) p53, the cellular gatekeeper for growth and division. Cell 88:323–331

    Article  PubMed  CAS  Google Scholar 

  11. Tosun M, Kalkan S (2002) İntrauterin Dönemde Apoptosis’in Önemi. Sendrom 14(3):95–104

    Google Scholar 

  12. Helal MA, Mehmet H, Thomas NSB, Cox PM, Ralph DJ, Bajoria R, Chatterjee R (2002) Ontogeny of human fetal testicular apoptosis during first, second, and third trimesters of pregnancy. J Clin Endocrinol Metab 87:1189–1193

    Article  PubMed  CAS  Google Scholar 

  13. Tosun M, Kalkan S (2002) Apoptosis ve Önemi. Sendrom 14(6):120–126

    Google Scholar 

  14. Diebel LN, Dulchavsky SA, Brown WJ (1997) Splanchnic ischemia and bacterial translocation in the abdominal compartment syndrome. J Trauma 43:852–855

    Article  PubMed  CAS  Google Scholar 

  15. Andersson L, Lagerstrand L, Thorne A, Sollevi A, Brodin LA, Odeberg-Wernermann S (2002) Effect of CO2 pneumoperitoneum on ventilation–perfusion relationships during laparoscopic cholecystectomy. Acta Anaesthesiol Scand 46:552–560

    Article  PubMed  CAS  Google Scholar 

  16. Gründel K, Böhm B, Bauwenss K, Junghans T, Schebia R (1998) Influence of acute hemorrhage and pneumoperitoneum on hemodynamic and respiratory parameters. Surg Endosc 12:809–812

    Article  PubMed  Google Scholar 

Download references

Acknowledgement

This study has been financed by Afyon Kocatepe University Scientific Research Projects Commission (approval no. 051-TIP-10).

Author information

Authors and Affiliations

  1. Medical Faculty Department of General Surgery, Afyon Kocatepe University, Afyonkarahisar, 03200, Turkey

    Yüksel Arikan & Volkan Şaykol

  2. Medical Faculty Department of Histology Embryology, Afyon Kocatepe University, Afyonkarahisar, 03200, Turkey

    Murat Tosun & Serpil Erdem

  3. Meram Medical Faculty Department of Histology Embryology, Selcuk University, Konya, 42080, Turkey

    Serpil Kalkan

  4. Medical Faculty Ali Cetinkaya kampusu, Afyon Kocatepe University, Afyon, Turkey

    Murat Tosun

Authors
  1. Yüksel Arikan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Murat Tosun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Volkan Şaykol
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Serpil Kalkan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Serpil Erdem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Murat Tosun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arikan, Y., Tosun, M., Şaykol, V. et al. p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum. Langenbecks Arch Surg 393, 877–882 (2008). https://doi.org/10.1007/s00423-007-0254-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00423-007-0254-6

Keywords

Search

Navigation