Abstract
Purpose
To determine associations between immediate and delayed response of serum cartilage oligomeric matrix protein (sCOMP) to loading (i.e., 3000 walking steps) and femoral cartilage interlimb T1ρ relaxation times in individual’s post-anterior cruciate ligament reconstruction (ACLR).
Methods
This cross-sectional study included 20 individuals 6–12 months following primary ACLR (65% female, 20.5 ± 4.0 years old, 24.9 ± 3.0 kg/m2, 7.3 ± 1.5 months post-ACLR). Serum samples were collected prior to, immediately following, and 3.5 h following walking 3000 steps on a treadmill at habitual walking speed. sCOMP concentrations were processed using enzyme-linked immunosorbent assays. Immediate and delayed absolute sCOMP responses to loading were evaluated immediately and 3.5 h post-walking, respectively. Participants underwent bilateral magnetic resonance imaging with T1ρ sequences to calculate resting femoral cartilage interlimb T1ρ relaxation time ratios between limbs (i.e., ACLR/Uninjured limb). Linear regression models were fitted to determine associations between sCOMP response to loading and femoral cartilage T1ρ outcomes controlling for pre-loading sCOMP concentrations.
Results
Greater increases in delayed sCOMP response to loading were associated with greater lateral (∆R2 = 0.29, p = 0.02) but not medial (∆R2 < 0.01, p = 0.99) femoral cartilage interlimb T1ρ ratios. Associations between immediate sCOMP response to loading with femoral cartilage interlimb T1ρ ratios were weak and non-significant (∆R2 range = 0.02–0.09, p range = 0.21–0.58).
Conclusion
Greater delayed sCOMP response to loading, a biomarker of cartilage breakdown, is associated with worse lateral femoral cartilage composition in the ACLR limb compared to the uninjured limb. Delayed sCOMP response to loading may be a more indicative metabolic indicator linked to deleterious changes in composition than immediate sCOMP response.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- ACL:
-
Anterior cruciate ligament
- ACLR:
-
Anterior cruciate ligament reconstruction
- BMI:
-
Body mass index
- COMP:
-
Cartilage oligomeric matrix protein
- ELISA:
-
Enzyme-linked immunosorbent assay
- KOOS:
-
Knee Injury Osteoarthritis Outcomes
- LFC:
-
Lateral femoral articular cartilage
- MRI:
-
Magnetic resonance imaging
- MFC:
-
Medial femoral articular cartilage
- PTOA:
-
Posttraumatic osteoarthritis
- sCOMP:
-
Serum cartilage oligomeric matrix protein
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Acknowledgements
We recognized the American College of Sports Medicine Doctoral Student Research Grant and the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health for funding this study.
Funding
This study was funded by the American College of Sports Medicine Doctoral Student Research Grant and the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health (1R21AR074094-01 and F31AR078013).
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CL and BP made substantial contributions to the concept and design of the manuscript as well as drafting of the work. CL, AEP, HDW, AEM, LL, DL, JRF, TAS and BP contributed to data analysis and interpretation. AEP, HDW, AEM, LL, DL, TAS, and JRF served as manuscript reviewer/revisers. AEP, HDW, AEM , LL, and DL aided in data acquisition.
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Lisee, C., Evans-Pickett, A., Davis-Wilson, H. et al. Delayed cartilage oligomeric matrix protein response to loading is associated with femoral cartilage composition post-ACLR. Eur J Appl Physiol 123, 2525–2535 (2023). https://doi.org/10.1007/s00421-023-05253-w
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DOI: https://doi.org/10.1007/s00421-023-05253-w