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The effect of prolonged interval and continuous exercise in the heat on circulatory markers of intestinal barrier integrity

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Abstract

Purpose

The purpose of this study was to determine the effect of prolonged high-intensity interval (INT) and moderate-intensity continuous (CONT) treadmill exercise in the heat on markers of enterocyte injury and bacterial endotoxin translocation.

Methods

Nine males completed 2 h of work-matched exercise in the heat (40 °C and 15% RH) as either INT (2 min at 80% VO2max and 3 min at 30% VO2max) or CONT (~ 50% of VO2max). Blood samples collected pre- and post-exercise were assayed for intestinal fatty acid-binding protein (I-FABP), claudin-3 (CLDN-3), and lipopolysaccharide-binding protein (LBP).

Results

I-FABP was significantly increased from pre- to post-exercise in CONT (913.96 ± 625.13 to 1477.26 ± 760.99 pg•mL−1; p = 0.014, d = 0.766) and INT (714.59 ± 470.27 to 1547.93 ± 760.99 pg•mL−1; p = 0.001, d = 1.160). Pre- to post-exercise changes in I-FABP were not different between CONT and INT (p = 0.088, d = 0.414). LBP was significantly increased from pre- to post-exercise in INT (15.94 ± 2.90 to 17.35 ± 3.26 μg•mL−1; p = 0.028, d = 0.459) but not CONT (18.11 ± 5.35 to 16.93 ± 5.39 μg•mL−1; p = 0.070, d = 0.226), and pre- to post-exercise changes in LBP were higher in the INT compared to CONT (p < 0.001, d = 1.160). No significant changes were detected from pre- to post-exercise for CLDN-3 in CONT (14.90 ± 2.21 to 15.30 ± 3.07 μg•mL−1) or INT (15.55 ± 1.63 to 16.41 ± 2.11 μg•mL−1) (p > 0.05).

Conclusions

We conclude that prolonged exercise in the heat induces enterocyte injury, but interval (or intermittent) exercise may cause greater bacterial endotoxin translocation which may increase the risk for local and systemic inflammation.

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Abbreviations

ηp 2 :

Partial eta squared

AUC:

Area under the curve

CLDN-3:

Claudin-3

CV:

Coefficient of variation

d:

Cohen’s d

ELISA:

Enzyme-linked immunosorbent assays

HR:

Heart rate

I-FABP:

Intestinal fatty acid-binding protein

LBP:

Lipopolysaccharide-binding protein

LPS:

Lipopolysaccharides

PSI:

Physiological strain index

RH:

Relative humidity

SD:

Standard deviation

Trectal :

Rectal temperature

USG:

Urine specific gravity

References

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Funding

Partial funding for this study was provided by the Graduate and Professional Student Association at the University of New Mexico.

Author information

Authors and Affiliations

Authors

Contributions

ZM, FA, and JH conceived the research. ZM, JH, CM, MD, OL, and FA designed the experiments. ZM, JH, JD, ZL, QB, ZF, AW, and FA collected data. ZM, JH, ZL, QB, CM, MD, OL, and FA analyzed data. ZM and JH drafted the manuscript. All authors contributed to the final manuscript.

Corresponding author

Correspondence to Zachary McKenna.

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Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This study was approved by the Institutional Review Board at the University of New Mexico Main Campus.

Consent to participate

All participants have read and signed an informed consent prior to participating in the study.

Additional information

Communicated by Narihiko kondo.

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McKenna, Z., Houck, J., Ducharme, J. et al. The effect of prolonged interval and continuous exercise in the heat on circulatory markers of intestinal barrier integrity. Eur J Appl Physiol 122, 2651–2659 (2022). https://doi.org/10.1007/s00421-022-05049-4

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  • DOI: https://doi.org/10.1007/s00421-022-05049-4

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