The aim of this study was to evaluate the short- and long-term effects of the Fasting-Mimicking-Diet (FMD) intervention on neuromuscular parameters of force production in healthy young men.
Twenty-four physically active men completed the study. Participants were randomly assigned to Fasting-Mimicking (FMD) or Normal Diet (ND) and asked to follow three cycles of dietary intervention. Neuromuscular parameters of force production during maximal voluntary isometric contractions (MVCs) with the leg extensors muscles and anthropometrics were measured at baseline (T0), at the end of the first cycle (T1), and 7–10 days after the 3rd cycle of the nutritional intervention (T2). The study was registered on Clinicaltrials.gov (No. NCT04476615).
There was a significant decrease in body mass at T1 for FMD (− 2.6 kg, ∆ from baseline, on average; p < 0.05) but not in ND (− 0.1 kg;). Neuromuscular parameters of force production, muscle volume, and MVC torque did not change or differ between groups across visits. Results were similar even when parameters were normalized by muscle volume.
The consumption of FMD in a group of young healthy male subjects showed to be feasible, and it did not affect neuromuscular parameters of force production. The results suggest that FMD could be safely adopted by strength athletes without detrimental effects on force and muscle volume. Further research in clinical population at risk of muscle mass loss, such as elderly and obese subjects with sarcopenia, is warranted.
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Analysis of variance
Body Mass Index
International physical activity questionnaire
Maximal rate of force development
Maximal rate of relaxation
Maximal voluntary isometric contraction
Peripheral nerve stimulation
- Qtwpot :
Potentiated single twitch
12-Item Short Form Health Survey
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This research was funded in part by the Create Cures Foundation.
Conflict of interest
VDL has equity interest in L-Nutra, a company that develops medical food. All other authors have no conflicts of interest to declare that are relevant to the content of this article.
The research protocol was approved by the protocol local ethical committee (No. 140166/2017) and conformed to the most recent revisions of the Declaration of Helsinki.
Informed consent was obtained from all the participants enrolled for this study.
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Communicated by Michalis G Nikolaidis.
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Nardon, M., Venturelli, M., Ruzzante, F. et al. Fasting-Mimicking-Diet does not reduce skeletal muscle function in healthy young adults: a randomized control trial. Eur J Appl Physiol 122, 651–661 (2022). https://doi.org/10.1007/s00421-021-04867-2