We investigated the consequence of varying hypoxia severity during an initial set of repeated cycling sprints on performance, neuromuscular fatigability, and exercise-related sensations during a subsequent set of repeated sprints in normoxia.
Nine active males performed ten 4-s sprints (recovery = 30 s) at sea level (SL; FiO2 ~ 0.21), moderate (MH; FiO2 ~ 0.17) or severe normobaric hypoxia (SH; FiO2 ~ 0.13). This was followed, after 8 min of passive recovery, by five 4-s sprints (recovery = 30 s) in normoxia.
Mean power decrement during Sprint 10 was exacerbated in SH compared to SL and MH (− 34 ± 12%, − 22 ± 13%, − 25 ± 14%, respectively, p < 0.05). Sprint performance during Sprint 11 recovered to that of Sprint 1 in all conditions (p = 0.267). All exercise-related sensations at Sprint 11 recovered significantly compared to Sprint 1, with no difference for Set 2 (p > 0.05). Ratings of overall perceived discomfort, difficulty breathing, and limb discomfort were exacerbated during Set 1 in SH versus SL (p < 0.05). Compared to SL, the averaged MPO value for Set 2 was 5.5 ± 3.0% (p = 0.003) lower in SH. Maximal voluntary force and twitch torque decreased similarly in all conditions immediately after Set 1 (p < 0.05), without further alterations after Set 2. Peripheral and cortical voluntary activation values did not change (p > 0.05).
Exercise-related sensations, rather than neuromuscular function integrity, may play a pivotal role in influencing performance of repeated sprints and its recovery.
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- FiO2 :
Fraction of inspired oxygen
Mean power output
Maximal voluntary contraction
Peripheral motor nerve
Root mean square
- SpO2 :
Arterial oxygen saturation
Transcranial magnetic stimulation
Amann M, Dempsey JA (2008) Locomotor muscle fatigue modifies central motor drive in healthy humans and imposes a limitation to exercise performance. J Physiol 586(1):161–173
Amann M, Kayser B (2009) Nervous system function during exercise in hypoxia. High Alt Med Biol 10(2):149–164
Amann M, Pegelow DF, Jacques AJ, Dempsey JA (2007a) Inspiratory muscle work in acute hypoxia influences locomotor muscle fatigue and exercise performance of healthy humans. Am J Physiol Regul Integr Comp Physiol 293(5):R2036–R2045
Amann M, Romer LM, Subudhi AW, Pegelow DF, Dempsey JA (2007b) Severity of arterial hypoxaemia affects the relative contributions of peripheral muscle fatigue to exercise performance in healthy humans. J Physiol 581(1):389–403
Arbogast S, Vassilakopoulos T, Darques JL, Duvauchelle JB, Jammes Y (2000) Influence of oxygen supply on activation of group IV muscle afferents after low-frequency muscle stimulation. Muscle Nerve 23(8):1187–1193
Billaut F, Bishop DJ (2012) Mechanical work accounts for sex differences in fatigue during repeated sprints. Eur J Appl Physiol 112(4):1429–1436
Billaut F, Kerris JP, Rodriguez RF, Martin DT, Gore CJ, Bishop DJ (2013) Interaction of central and peripheral factors during repeated sprints at different levels of arterial O2 saturation. PLoS ONE 8(10):e77297
Bishop D, Lawrence S, Spencer M (2003) Predictors of repeated-sprint ability in elite female hockey players. J Sci Med Sport 6(2):199–209
Brocherie F, Girard O, Faiss R, Millet GP (2017) Effects of repeated-sprint training in hypoxia on sea-level performance: a meta-analysis. Sports Med 47(8):1651–1660. https://doi.org/10.1007/s40279-017-0685-3
Christian RJ, Bishop D, Girard O, Billaut F (2014) The role of sense of effort on self-selected cycling power output. Front Physiol 5:115
Collins BW, Pearcey GE, Buckle NC, Power KE, Button DC (2018) Neuromuscular fatigue during repeated sprint exercise: underlying physiology and methodological considerations. Appl Physiol Nutr Metab 43(11):1166–1175
Del Vecchio A, Negro F, Felici F, Farina D (2017) Associations between motor unit action potential parameters and surface EMG features. J Appl Physiol 123(4):835–843
Enoka RM, Duchateau J (2016) Translating fatigue to human performance. Med Sci Sports Exerc 48(11):2228
Fernández-Pena E, Lucertini F, Ditroilo M (2009) A maximal isokinetic pedalling exercise for EMG normalization in cycling. J Electromyogr Kinesiol 19(3):e162–e170
Gandevia SC (2001) Spinal and supraspinal factors in human muscle fatigue. Physiol Rev 81(4):1725–1789
Girard O, Mendez-Villanueva A, Bishop D (2011) Repeated-sprint ability—Part I. Sports Med 41(8):673–694
Girard O, Bishop DJ, Racinais S (2013) Neuromuscular adjustments of the quadriceps muscle after repeated cycling sprints. PLoS ONE 8(5):e61793
Girard O, Brocherie F, Morin J-B, Millet GP (2015) Neuro-mechanical determinants of repeated treadmill sprints-Usefulness of an “hypoxic to normoxic recovery” approach. Front Physiol 6:260
Girard O, Billaut F, Christian RJ, Bradley PS, Bishop DJ (2017) Exercise-related sensations contribute to decrease power during repeated cycle sprints with limited influence on neural drive. Eur J Appl Physiol 117(11):2171–2179
Goodall S, Romer L, Ross E (2009) Voluntary activation of human knee extensors measured using transcranial magnetic stimulation. Exp Physiol 94(9):995–1004
Goodall S, González-Alonso J, Ali L, Ross EZ, Romer LM (2012) Supraspinal fatigue after normoxic and hypoxic exercise in humans. J Physiol 590(11):2767–2782
Goodall S, Charlton K, Howatson G, Thomas K (2015) Neuromuscular fatigability during repeated-sprint exercise in male athletes. Med Sci Sports Exerc 47(3):528–536
Goods PS, Dawson BT, Landers GJ, Gore CJ, Peeling P (2014) Effect of different simulated altitudes on repeat-sprint performance in team-sport athletes. Int J Sports Physiol Perform 9(5):857–862
Herbert R, Gandevia S (1999) Twitch interpolation in human muscles: mechanisms and implications for measurement of voluntary activation. J Neurophysiol 82(5):2271–2283
Hureau TJ, Olivier N, Millet GY, Meste O, Blain GM (2014) Exercise performance is regulated during repeated sprints to limit the development of peripheral fatigue beyond a critical threshold. Exp Physiol 99(7):951–963
Hureau TJ, Ducrocq GP, Blain GM (2016) Peripheral and central fatigue development during all-out repeated cycling sprints. Med Sci Sports Exerc 48(3):391–401
Karatzaferi C, De Haan A, Van Mechelen W, Sargeant A (2001) Metabolic changes in single human muscle fibres during brief maximal exercise. Exp Physiol 86(3):411–415
Marcora SM, Staiano W (2010) The limit to exercise tolerance in humans: mind over muscle? Eur J Appl Physiol 109(4):763–770
Martin J, Spirduso W (2001) Determinants of maximal cycling power: crank length, pedaling rate and pedal speed. Eur J Appl Physiol 84(5):413–418
Mendez-Villanueva A, Hamer P, Bishop D (2007) Fatigue responses during repeated sprints matched for initial mechanical output. Med Sci Sports Exerc 39(12):2219–2225
Mendez-Villanueva A, Edge J, Suriano R, Hamer P, Bishop D (2012) The recovery of repeated-sprint exercise is associated with PCr resynthesis, while muscle pH and EMG amplitude remain depressed. PLoS ONE 7(12):e51977
Millet GY, Muthalib M, Jubeau M, Laursen PB, Nosaka K (2012) Severe hypoxia affects exercise performance independently of afferent feedback and peripheral fatigue. J Appl Physiol 112(8):1335–1344
Noakes TDO (2012) Fatigue is a brain-derived emotion that regulates the exercise behavior to ensure the protection of whole body homeostasis. Front Physiol 3:82
Pearcey GE, Murphy JR, Behm DG, Hay DC, Power KE, Button DC (2015) Neuromuscular fatigue of the knee extensors during repeated maximal intensity intermittent-sprints on a cycle ergometer. Muscle Nerve 51(4):569–579
Perrey S, Rupp T (2009) Altitude-induced changes in muscle contractile properties. High Alt Med Biol 10(2):175–182
Rothwell J (1997) Techniques and mechanisms of action of transcranial stimulation of the human motor cortex. J Neurosci Methods 74(2):113–122
Sidhu SK, Bentley DJ, Carroll TJ (2009) Cortical voluntary activation of the human knee extensors can be reliably estimated using transcranial magnetic stimulation. Muscle Nerve 39(2):186–196
Sweeting AJ, Billaut F, Varley MC, Rodriguez RF, Hopkins WG, Aughey RJ (2017) Variations in hypoxia impairs muscle oxygenation and performance during simulated team-sport running. Front Physiol 8:80
Taylor JL (2009) Point: counterpoint: the interpolated twitch does/does not provide a valid measure of the voluntary activation of muscle. J Appl Physiol 107(1):354–355
Todd G, Taylor JL, Butler JE, Martin PG, Gorman RB, Gandevia SC (2007) Use of motor cortex stimulation to measure simultaneously the changes in dynamic muscle properties and voluntary activation in human muscles. J Appl Physiol 102(5):1756–1766
Verges S, Maffiuletti NA, Kerherve H, Decorte N, Wuyam B, Millet GY (2009) Comparison of electrical and magnetic stimulations to assess quadriceps muscle function. J Appl Physiol 106(2):701–710
The authors thank all the subjects for their participation in this study.
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Communicated by Guido Ferretti.
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Soo, J., Billaut, F., Bishop, D.J. et al. Neuromuscular and perceptual responses during repeated cycling sprints—usefulness of a “hypoxic to normoxic” recovery approach. Eur J Appl Physiol 120, 883–896 (2020). https://doi.org/10.1007/s00421-020-04327-3
- Repeated-sprint ability
- Exercise-related sensations
- Neuromuscular fatigue