Whole-body vibration (WBV) training is frequently applied in sports and rehabilitation with the aim of inducing beneficial functional and structural adaptations. In the past decades, blood flow restriction (BFR) training has received increasing attention by enhancing the effectiveness of several low-load exercise regimens. The objective of this study was to evaluate the additional effect of BFR on myoelectric activity and metabolic accumulation during WBV training.
Fifteen active men performed three sessions in a counterbalanced order on three different days: whole-body vibration exercise (WBV), whole-body vibration exercise with blood flow restriction (WBV + BFR), and a control session (CON) with neither WBV nor BFR. Electromyographic (EMG) activity was measured in six lower limb muscles throughout each exercise session; lactate and reactive oxygen species (ROS) concentrations were determined prior to, immediately after and 15 min after the exercise sessions.
EMG amplitudes increased from CON (29 ± 13% MVC) to WBV (45 ± 20% MVC) to WBV + BFR (71 ± 37% MVC) conditions (p < 0.05). Likewise, lactate concentrations increased in a similar manner, demonstrating significantly higher increases in the WBV + BFR session compared to WBV and CON. Furthermore, significant correlations between lactate concentration and EMG amplitude were detected. ROS concentration did not change significantly between the conditions.
The findings of the present study emphasize that the addition of BFR increases the acute effects beyond WBV treatment alone which becomes manifested in both neuromuscular and metabolic adaptations. Further research is needed to identify potential long-term effects of the combination of these two training regimens.
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One repetition maximum
Arterial occlusion pressure
Blood flow restriction
Electron paramagnetic resonance
Maximum voluntary contraction
Root mean square
Reactive oxygen species
Aagaard P et al (2001) A mechanism for increased contractile strength of human pennate muscle in response to strength training: changes in muscle architecture. J Physiol 534:613–623
Abercromby AF, Amonette WE, Layne CS, McFarlin BK, Hinman MR, Paloski WH (2007) Variation in neuromuscular responses during acute whole-body vibration exercise. Med Sci Sports Exerc 39:1642–1650. https://doi.org/10.1249/mss.0b013e318093f551
Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL (2000) Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 32:1576–1581
Bittar ST, Pfeiffer PS, Santos HH, Cirilo-Sousa MS (2018) Effects of blood flow restriction exercises on bone metabolism: a systematic review. Clin Physiol Funct Imaging. https://doi.org/10.1111/cpf.12512
Bogaerts A, Delecluse C, Claessens AL, Coudyzer W, Boonen S, Verschueren SM (2007) Impact of whole-body vibration training versus fitness training on muscle strength and muscle mass in older men: a 1-year randomized controlled trial. J Gerontol A Biol Sci Med Sci 62:630–635
Cardinale M, Lim J (2003) Electromyography activity of vastus lateralis muscle during whole-body vibrations of different frequencies. J Strength Cond Res 17:621–624
Centner C, Wiegel P, Gollhofer A, König D (2019) Effects of blood flow restriction training on muscular strength and hypertrophy in older individuals: a systematic review and meta-analysis. Sports Med 49:95–108. https://doi.org/10.1007/s40279-018-0994-1
Chen WC, Wu CM, Cai ZY (2018) Effect of one bout of local vibration exercise with blood flow restriction on neuromuscular and hormonal responses. Physiol Int 105:166–176. https://doi.org/10.1556/2060.105.2018.2.9
Cochrane DJ, Stannard SR (2005) Acute whole body vibration training increases vertical jump and flexibility performance in elite female field hockey players. Br J Sports Med 39:860
Cook SB, Kanaley JA, Ploutz-Snyder LL (2014) Neuromuscular function following muscular unloading and blood flow restricted exercise. Eur J Appl Physiol 114:1357–1365. https://doi.org/10.1007/s00421-014-2864-3
Debold EP (2012) Recent insights into the molecular basis of muscular fatigue. Med Sci Sport Exerc 44:1440–1452. https://doi.org/10.1249/mss.0b013e31824cfd26
Delecluse C, Roelants M, Verschueren S (2003) Strength increase after whole-body vibration compared with resistance training. Med Sci Sports Exerc 35:1033–1041. https://doi.org/10.1249/01.MSS.0000069752.96438.B0
Droge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47–95. https://doi.org/10.1152/physrev.00018.2001
Ebing J, Gast U, Hauptmann C, Felsenberg D, Belavy DL (2018) Hypertrophy and explosive-reactive functioning in sedentary men after 10 weeks of whole-body vibration. J Strength Cond Res 32:27–36. https://doi.org/10.1519/jsc.0000000000001728
Figueroa A, Gil R, Wong A, Hooshmand S, Park SY, Vicil F, Sanchez-Gonzalez MA (2012) Whole-body vibration training reduces arterial stiffness, blood pressure and sympathovagal balance in young overweight/obese women. Hypertens Res 35:667–672. https://doi.org/10.1038/hr.2012.15
Finaud J, Lac G, Filaire E (2006) Oxidative stress: relationship with exercise and training. Sports Med 36:327–358
Fry CS et al (2010) Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men. J Appl Physiol 108:1199–1209. https://doi.org/10.1152/japplphysiol.01266.2009
Fujita S et al (2007) Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. J Appl Physiol 103:903–910. https://doi.org/10.1152/japplphysiol.00195.2007
Giles L, Webster KE, McClelland J, Cook JL (2017) Quadriceps strengthening with and without blood flow restriction in the treatment of patellofemoral pain: a double-blind randomised trial. Br J Sports Med 51:1688. https://doi.org/10.1136/bjsports-2016-096329
Gómez-Bruton A et al (2017) Do 6 months of whole-body vibration training improve lean mass and bone mass acquisition of adolescent swimmers? Arch Osteoporos 12:69. https://doi.org/10.1007/s11657-017-0362-z
Henneman E, Somjen G, Carpenter DO (1965) Functional significance of cell size in spinal motoneurons. J Neurophysiol 28:560–580
Hughes L, Paton B, Rosenblatt B, Gissane C, Patterson SD (2017) Blood flow restriction training in clinical musculoskeletal rehabilitation: a systematic review and meta-analysis. Br J Sports Med 51:1003–1011. https://doi.org/10.1136/bjsports-2016-097071
Item F, Nocito A, Thony S, Bachler T, Boutellier U, Wenger RH, Toigo M (2013) Combined whole-body vibration, resistance exercise, and sustained vascular occlusion increases PGC-1alpha and VEGF mRNA abundances. Eur J Appl Physiol 113:1081–1090. https://doi.org/10.1007/s00421-012-2524-4
Karabulut M, Cramer JT, Abe T, Sato Y, Bemben MG (2010) Neuromuscular fatigue following low-intensity dynamic exercise with externally applied vascular restriction. J Electromyogr Kinesiol 20:440–447. https://doi.org/10.1016/j.jelekin.2009.06.005
Lixandrao ME et al (2018) Magnitude of muscle strength and mass adaptations between high-load resistance training versus low-load resistance training associated with blood-flow restriction: a systematic review and meta-analysis. Sports Med 48:361–378. https://doi.org/10.1007/s40279-017-0795-y
Loenneke JP, Fahs CA, Wilson JM, Bemben MG (2011) Blood flow restriction: the metabolite/volume threshold theory. Med Hypotheses 77:748–752. https://doi.org/10.1016/j.mehy.2011.07.029
Loenneke JP, Fahs CA, Rossow LM, Abe T, Bemben MG (2012a) The anabolic benefits of venous blood flow restriction training may be induced by muscle cell swelling. Med Hypotheses 78:151–154. https://doi.org/10.1016/j.mehy.2011.10.014
Loenneke JP, Wilson JM, Marin PJ, Zourdos MC, Bemben MG (2012b) Low intensity blood flow restriction training: a meta-analysis. Eur J Appl Physiol 112:1849–1859. https://doi.org/10.1007/s00421-011-2167-x
Loenneke JP, Thiebaud RS, Fahs CA, Rossow LM, Abe T, Bemben MG (2013) Blood flow restriction does not result in prolonged decrements in torque. Eur J Appl Physiol 113:923–931. https://doi.org/10.1007/s00421-012-2502-x
Loenneke JP et al (2015) Effects of exercise with and without different degrees of blood flow restriction on torque and muscle activation. Muscle Nerve 51:713–721. https://doi.org/10.1002/mus.24448
Machado A, Garcia-Lopez D, Gonzalez-Gallego J, Garatachea N (2010) Whole-body vibration training increases muscle strength and mass in older women: a randomized-controlled trial. Scand J Med Sci Sports 20:200–207. https://doi.org/10.1111/j.1600-0838.2009.00919.x
Mariappan N, Elks CM, Fink B, Francis J (2009) TNF-induced mitochondrial damage: a link between mitochondrial complex I activity and left ventricular dysfunction. Free Radic Biol Med 46:462–470. https://doi.org/10.1016/j.freeradbiomed.2008.10.049
Marin PJ, Rhea MR (2010) Effects of vibration training on muscle strength: a meta-analysis. J Strength Cond Res 24:548–556. https://doi.org/10.1519/JSC.0b013e3181c09d22
Marshall LC, Wyon MA (2012) The effect of whole-body vibration on jump height and active range of movement in female dancers. J Strength Cond Res 26:789–793. https://doi.org/10.1519/JSC.0b013e31822a5ce8
Masumoto K, Takasugi S, Hotta N, Fujishima K, Iwamoto Y (2004) Electromyographic analysis of walking in water in healthy humans. J Physiol Anthropol Appl Hum Sci 23:119–127
Moritani T, Sherman WM, Shibata M, Matsumoto T, Shinohara M (1992) Oxygen availability and motor unit activity in humans. Eur J Appl Physiol Occup Physiol 64:552–556
Mrakic-Sposta S, Gussoni M, Montorsi M, Porcelli S, Vezzoli A (2012) Assessment of a standardized ROS production profile in humans by electron paramagnetic resonance. Oxid Med Cell Longev 2012:973927. https://doi.org/10.1155/2012/973927
Nielsen JL, Aagaard P, Prokhorova TA, Nygaard T, Bech RD, Suetta C, Frandsen U (2017) Blood flow restricted training leads to myocellular macrophage infiltration and upregulation of heat shock proteins, but no apparent muscle damage. J Physiol 595:4857–4873. https://doi.org/10.1113/jp273907
Patterson SD, Ferguson RA (2010) Increase in calf post-occlusive blood flow and strength following short-term resistance exercise training with blood flow restriction in young women. Eur J Appl Physiol 108:1025–1033. https://doi.org/10.1007/s00421-009-1309-x
Pearson SJ, Hussain SR (2015) A review on the mechanisms of blood-flow restriction resistance training-induced muscle hypertrophy. Sports Med 45:187–200. https://doi.org/10.1007/s40279-014-0264-9
Perchthaler D, Grau S, Hein T (2015) Evaluation of a six-week whole-body vibration intervention on neuromuscular performance in older adults. J Strength Cond Res 29:86–95. https://doi.org/10.1519/JSC.0000000000000608
Pollock RD, Woledge RC, Mills KR, Martin FC, Newham DJ (2010) Muscle activity and acceleration during whole body vibration: effect of frequency and amplitude. Clin Biomech 25:840–846. https://doi.org/10.1016/j.clinbiomech.2010.05.004
Powers SK, Duarte J, Kavazis AN, Talbert EE (2010) Reactive oxygen species are signalling molecules for skeletal muscle adaptation. Exp Physiol 95:1–9. https://doi.org/10.1113/expphysiol.2009.050526
Powers SK, Ji LL, Kavazis AN, Jackson MJ (2011a) Reactive oxygen species: impact on skeletal muscle. Compr Physiol 1:941–969. https://doi.org/10.1002/cphy.c100054
Powers SK, Talbert EE, Adhihetty PJ (2011b) Reactive oxygen and nitrogen species as intracellular signals in skeletal muscle. J Physiol 589:2129–2138. https://doi.org/10.1113/jphysiol.2010.201327
Reeves GV, Kraemer RR, Hollander DB, Clavier J, Thomas C, Francois M, Castracane VD (2006) Comparison of hormone responses following light resistance exercise with partial vascular occlusion and moderately difficult resistance exercise without occlusion. J Appl Physiol 101:1616–1622. https://doi.org/10.1152/japplphysiol.00440.2006
Ritzmann R, Gollhofer A, Kramer A (2013) The influence of vibration type, frequency, body position and additional load on the neuromuscular activity during whole body vibration. Eur J Appl Physiol 113:1–11. https://doi.org/10.1007/s00421-012-2402-0
Ritzmann R, Kramer A, Bernhardt S, Gollhofer A (2014) Whole body vibration training–improving balance control and muscle endurance. PLoS One 9:e89905. https://doi.org/10.1371/journal.pone.0089905
Roelants M, Verschueren SM, Delecluse C, Levin O, Stijnen V (2006) Whole-body-vibration-induced increase in leg muscle activity during different squat exercises. J Strength Cond Res 20:124–129. https://doi.org/10.1519/r-16674.1
Sauer H, Wartenberg M, Hescheler J (2001) Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell Physiol Biochem 11:173–186. https://doi.org/10.1159/000047804
Schoenfeld BJ (2013) Potential mechanisms for a role of metabolic stress in hypertrophic adaptations to resistance training. Sports Med 43:179–194. https://doi.org/10.1007/s40279-013-0017-1
Scott BR, Loenneke JP, Slattery KM, Dascombe BJ (2015) Exercise with blood flow restriction: an updated evidence-based approach for enhanced muscular development. Sports Med 45:313–325. https://doi.org/10.1007/s40279-014-0288-1
Slysz J, Stultz J, Burr JF (2016) The efficacy of blood flow restricted exercise: a systematic review and meta-analysis. J Sci Med Sport 19:669–675. https://doi.org/10.1016/j.jsams.2015.09.005
Suga T et al (2012) Effect of multiple set on intramuscular metabolic stress during low-intensity resistance exercise with blood flow restriction. Eur J Appl Physiol 112:3915–3920. https://doi.org/10.1007/s00421-012-2377-x
Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N (2000a) Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol 88:61–65
Takarada Y, Takazawa H, Sato Y, Takebayashi S, Tanaka Y, Ishii N (2000b) Effects of resistance exercise combined with moderate vascular occlusion on muscular function in humans. J Appl Physiol (1985) 88:2097–2106
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84. https://doi.org/10.1016/j.biocel.2006.07.001
Verschueren SM, Roelants M, Delecluse C, Swinnen S, Vanderschueren D, Boonen S (2004) Effect of 6-month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. J Bone Miner Res 19:352–359. https://doi.org/10.1359/jbmr.0301245
Ward K, Alsop C, Caulton J, Rubin C, Adams J, Mughal Z (2004) Low magnitude mechanical loading is osteogenic in children with disabling conditions. J Bone Miner Res 19:360–369. https://doi.org/10.1359/JBMR.040129
Wiley ME, Damiano DL (1998) Lower-extremity strength profiles in spastic cerebral palsy. Dev Med Child Neurol 40:100–107
Yasuda T, Brechue WF, Fujita T, Shirakawa J, Sato Y, Abe T (2009) Muscle activation during low-intensity muscle contractions with restricted blood flow. J Sports Sci 27:479–489. https://doi.org/10.1080/02640410802626567
Yasuda T et al (2010) Venous blood gas and metabolite response to low-intensity muscle contractions with external limb compression. Metabolism 59:1510–1519. https://doi.org/10.1016/j.metabol.2010.01.016
Yasuda T et al (2014) Effects of low-intensity, elastic band resistance exercise combined with blood flow restriction on muscle activation. Scand J Med Sci Sports 24:55–61. https://doi.org/10.1111/j.1600-0838.2012.01489.x
Yasuda T et al (2015) Effects of low-load, elastic band resistance training combined with blood flow restriction on muscle size and arterial stiffness in older adults. J Gerontol A Biol Sci Med Sci 70:950–958. https://doi.org/10.1093/gerona/glu084
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Communicated by Michalis G. Nikolaidis.
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Centner, C., Ritzmann, R., Schur, S. et al. Blood flow restriction increases myoelectric activity and metabolic accumulation during whole-body vibration. Eur J Appl Physiol 119, 1439–1449 (2019). https://doi.org/10.1007/s00421-019-04134-5
- Blood flow restriction
- Metabolic accumulation
- Whole-body vibration
- Myoelectric activity