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Blunted satellite cell response is associated with dysregulated IGF-1 expression after exercise with age

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Abstract

Purpose

Insulin-like growth factor-1 (IGF-1) regulates protein synthesis and cell cycle kinetics. Given that aging is associated with anabolic resistance, we sought to determine if the attenuated exercise-induced satellite cell (SC) expression in older muscle is associated with a blunted IGF-1 response.

Methods

SC expression (Pax7+ cells) and protein (Western blot) and mRNA (RT-PCR) expression of IGF-1 splice variants and ubiquitous (IGFBP4) and muscle-specific (IGFBP3 and -5) IGF-1 binding proteins were measured in skeletal muscle of young (Y: 22 ± 2, n = 7) and older (O: 70 ± 2, n = 7) adults up to 48 h after an acute bout of resistance exercise.

Results

SC expression was greater in Y compared to O (age; P < 0.01) and increased (interaction; P < 0.05) by 24 h after exercise in Y only. IGF-1Ea and IGF-1Eb mRNA tended to be greater in O (age; P < 0.06–0.09). IGF-1Eb mRNA increased at 48 h (time; P < 0.05), whereas IGF-1Ec mRNA increased (interaction; P < 0.05) at 24 and 48 h in O only. IGF binding protein (IGFBP)4 mRNA was greater (age; P < 0.01) in O with the increase at 24 h and 48 h (time; P < 0.01) primarily driven by changes in O (interaction; P < 0.01). Despite IGFBP3 mRNA being greater in O (age; P < 0.01) and increasing at 48 h (time; P < 0.01), there was no effect of age or exercise on IGFBP3 protein expression. In contrast, IGFBP5 mRNA was greater (age; P < 0.01) despite IGFBP5 protein expression being lower (age; P < 0.01) in O compared to Y.

Conclusions

The greater muscle-specific expression of IGF-1 family members with a blunted post-exercise SC expression may be a compensatory attempt to rescue age-related anabolic resistance.

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Abbreviations

IGF-1:

Insulin-like growth factor-1

IGFBP:

Insulin-like growth factor-1 binding protein

JAK/STAT:

Janus tyrosine kinase/signal transducer and activator of transcription

mRNA:

Messenger ribonucleic acid

Pax7:

Paired box transcription factor 7

SC:

Satellite cells

SOCS:

Suppressor of cytokine signaling

TBST:

Tris-buffered saline with 0.1% Tween

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Acknowledgements

Study funding provided by a Natural Sciences and Engineering Research Council (NSERC) Discovery Grant to GP. DRM was supported in part by an NSERC Post-Doctoral Fellowship.

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Authors and Affiliations

  1. Faculty of Kinesiology and Physical Education, University of Toronto, 100 Devonshire Place, Toronto, ON, M5S 2W6, Canada

    Daniel R. Moore

  2. Department of Kinesiology, McMaster University, Hamilton, ON, Canada

    Daniel R. Moore, Bryon R. McKay & Gianni Parise

  3. Department of Pediatrics and Medicine, McMaster University, Hamilton, ON, Canada

    Mark A. Tarnopolsky

Authors
  1. Daniel R. Moore
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  2. Bryon R. McKay
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  3. Mark A. Tarnopolsky
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  4. Gianni Parise
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Contributions

BRM, MAT, and GP designed the research. BRM, MAT, and GP conducted the study. DRM and BRM analyzed the data. DRM wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Daniel R. Moore.

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Authors declare that they have no conflict of interest

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Communicated by William J. Kraemer.

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Moore, D.R., McKay, B.R., Tarnopolsky, M.A. et al. Blunted satellite cell response is associated with dysregulated IGF-1 expression after exercise with age. Eur J Appl Physiol 118, 2225–2231 (2018). https://doi.org/10.1007/s00421-018-3954-4

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  • DOI: https://doi.org/10.1007/s00421-018-3954-4

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