Abstract
Purpose
The effect of carbohydrate (CHO), or CHO supplemented with either sodium caseinate protein (CHO–C) or a sodium caseinate protein hydrolysate (CHO–H) on the recovery of skeletal muscle glycogen and anabolic signaling following prolonged aerobic exercise was determined in trained male cyclists [n = 11, mean ± SEM age 28.8 ± 2.3 years; body mass (BM) 75.0 ± 2.3 kg; VO2peak 61.3 ± 1.6 ml kg−1 min−1].
Methods
On three separate occasions, participants cycled for 2 h at ~ 70% VO2peak followed by a 4-h recovery period. Isoenergetic drinks were consumed at + 0 and + 2 h of recovery containing either (1) CHO (1.2 g kg −1 BM), (2) CHO–C, or (3) CHO–H (1.04 and 0.16 g kg−1 BM, respectively) in a randomized, double-blind, cross-over design. Muscle biopsies from the vastus lateralis were taken prior to commencement of each trial, and at + 0 and + 4 h of recovery for determination of skeletal muscle glycogen, and intracellular signaling associated with protein synthesis.
Results
Despite an augmented insulin response following CHO–H ingestion, there was no significant difference in skeletal muscle glycogen resynthesis following recovery between trials. CHO–C and CHO–H co-ingestion significantly increased phospho-mTOR Ser2448 and 4EBP1 Thr37/46 versus CHO, with CHO–H displaying the greatest change in phospho-4EBP1 Thr37/46. Protein co-ingestion, compared to CHO alone, during recovery did not augment glycogen resynthesis.
Conclusion
Supplementing CHO with intact sodium caseinate or an insulinotropic hydrolysate derivative augmented intracellular signaling associated with skeletal muscle protein synthesis following prolonged aerobic exercise.
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Abbreviations
- 4EBP1:
-
4E binding protein 1
- AA:
-
Amino acids
- BCAA:
-
Branched chain amino acids
- BM:
-
Body mass
- BMI:
-
Body mass index
- CHO:
-
Carbohydrate
- CHO–C:
-
CHO and sodium caseinate
- CHO–H:
-
CHO and sodium caseinate hydrolysate
- C max :
-
Maximal plasma concentration
- DPP-IV:
-
Dipeptidyl peptidase 4
- DXA:
-
Dual energy X-ray absorptiometry
- EAA:
-
Essential amino acids
- eEF2:
-
Eukaryotic elongation factor 2
- GAPDH:
-
Glyceraldehyde 3-phosphate dehydrogenase
- HPLC:
-
High performance liquid chromatography
- HR:
-
Heart rate
- HRmax :
-
Maximum heart rate
- LT:
-
Lactate threshold
- MPS:
-
Muscle protein synthesis
- mTOR:
-
Mechanistic target of rapamycin
- OPA:
-
O-phthalaldehyde
- RER:
-
Respiratory exchange ratio
- RPE:
-
Rating of perceived exertion
- RP-UPLC:
-
Reverse phase ultra-performance liquid chromatography
- TAA:
-
Total amino acids
- VO2peak :
-
Peak oxygen consumption
- W max :
-
Maximum power output
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Acknowledgements
Dr. Will McCormack and Prof. Phil Jakeman (University of Limerick, Ireland) for technical assistance with the analysis of plasma amino acid concentrations.
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This work was supported by Food for Health Ireland (F.H.I) and Enterprise Ireland (Grant No.: TC2013001).
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The authors declare no conflict of interests.
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Communicated by Anni Vanhatalo.
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Cogan, K.E., Evans, M., Iuliano, E. et al. Co-ingestion of protein or a protein hydrolysate with carbohydrate enhances anabolic signaling, but not glycogen resynthesis, following recovery from prolonged aerobic exercise in trained cyclists. Eur J Appl Physiol 118, 349–359 (2018). https://doi.org/10.1007/s00421-017-3775-x
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DOI: https://doi.org/10.1007/s00421-017-3775-x