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European Journal of Applied Physiology

, Volume 116, Issue 2, pp 311–318 | Cite as

The effect of post-resistance exercise alcohol ingestion on lipopolysaccharide-stimulated cytokines

  • Danielle E. Levitt
  • Anthony A. Duplanty
  • Ronald G. BudnarJr.
  • Hui-Ying Luk
  • Alexander Fernandez
  • Travis J. Layman
  • Daniel L. Fancher
  • David W. Hill
  • Brian K. McFarlin
  • Jakob L. VingrenEmail author
Original Article

Abstract

Purpose

To examine the effect of post-resistance exercise alcohol ingestion on lipopolysaccharide (LPS)-stimulated production of IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10.

Methods

Recreationally resistance-trained men (n = 10, 25 ± 3 year, 177 ± 7 cm, 83.8 ± 15.7 kg, 14.8 ± 8.5 % body fat) and women (n = 8, 23 ± 2 year, 161 ± 3 cm, 59.5 ± 6.0 kg, 26.5 ± 3.0 % body fat) completed two identical heavy back squat sessions (6 × 10 at 80 % 1 repetition maximum) followed by ingestion of either an alcohol (ALC; 1.09 g ethanol ·  kg fat-free mass−1) or water (PLA) drink. Blood samples were collected before exercise (PRE), and at 3 h (3 h), and 5 h (5 h) after exercise, stimulated with LPS, and analyzed for IFNγ, TNF-α, IL-1β, IL-6, IL-8, and IL-10 concentrations.

Results

There were no drink conditions by time effects for IFNγ, TNF-α, IL-1β, or IL-10. Regardless of condition, resistance exercise induce an increase in IFNγ, TNF-α, and IL-1β at 5 h compared to PRE but a decrease in IL-10 at 3 and 5 h compared to PRE. For ALC, IL-8 was reduced at 5 h compared to PLA. From PRE to 3 h, IL-6 was reduced for ALC but increased for PLA; resistance exercise induced an increase in IL-6 for both conditions at 5 h.

Conclusions

Heavy resistance exercise increased production of IFNγ, TNF-α, IL-1β, and Il-6 and decreased production of IL-10. Alcohol ingestion after resistance exercise affected aspects of inflammatory capacity (IL-6 and IL-8 production). It appears that some of the effects previously observed for alcohol ingestion alone on the LPS-stimulated cytokine production were overwhelmed by the response to resistance exercise.

Keywords

Resistance exercise Alcohol Lipopolysaccharide Inflammation 

Abbreviations

1RM

One-repetition maximum

3 h

Three hours post-exercise time point

5 h

Five hours post-exercise time point

ALC

Alcohol condition

ANOVA

Analysis of variance

BAC

Blood alcohol concentration

IFN

Interferon

IL

Interleukin

LPS

Lipopolysaccharide

PLA

Placebo condition

PRE

Pre-exercise time point

TNF

Tumor necrosis factor

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Funding

This project was funded in part by grants from the American College of Sports Medicine—Texas Chapter and the National Strength and Conditioning Association.

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the Institutional Review Board of the University of North Texas and in accordance with the 1964 Helsinki declaration and its later amendments. This article does not contain any studies with animals performed by the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Danielle E. Levitt
    • 1
    • 2
  • Anthony A. Duplanty
    • 1
    • 2
  • Ronald G. BudnarJr.
    • 1
    • 2
  • Hui-Ying Luk
    • 1
    • 2
  • Alexander Fernandez
    • 1
  • Travis J. Layman
    • 1
  • Daniel L. Fancher
    • 1
  • David W. Hill
    • 1
  • Brian K. McFarlin
    • 1
    • 2
  • Jakob L. Vingren
    • 1
    • 2
    Email author
  1. 1.Applied Physiology Laboratory, Department of Kinesiology, Health Promotion, and RecreationUniversity of North TexasDentonUSA
  2. 2.Department of Biological SciencesUniversity of North TexasDentonUSA

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