Neuro-mechanical and metabolic adjustments to the repeated anaerobic sprint test in professional football players

Abstract

Purpose

This study aimed to determine the neuro-mechanical and metabolic adjustments in the lower limbs induced by the running anaerobic sprint test (the so-called RAST).

Methods

Eight professional football players performed 6 × 35 m sprints interspersed with 10 s of active recovery on artificial turf with their football shoes. Sprinting mechanics (plantar pressure insoles), root mean square activity of the vastus lateralis (VL), rectus femoris (RF), and biceps femoris (BF) muscles (surface electromyography, EMG) and VL muscle oxygenation (near-infrared spectroscopy) were monitored continuously.

Results

Sprint time, contact time and total stride duration increased from the first to the last repetition (+17.4, +20.0 and +16.6 %; all P < 0.05), while flight time and stride length remained constant. Stride frequency (−13.9 %; P < 0.001) and vertical stiffness decreased (−27.2 %; P < 0.001) across trials. Root mean square EMG activities of RF and BF (−18.7 and −18.1 %; P < 0.01 and 0.001, respectively), but not VL (−1.2 %; P > 0.05), decreased over sprint repetitions and were correlated with the increase in running time (r = −0.82 and −0.90; both P < 0.05). Together with a better maintenance of RF and BF muscles activation levels over sprint repetitions, players with a better repeated-sprint performance (lower cumulated times) also displayed faster muscle de- (during sprints) and re-oxygenation (during recovery) rates (r = −0.74 and −0.84; P < 0.05 and 0.01, respectively).

Conclusion

The repeated anaerobic sprint test leads to substantial alterations in stride mechanics and leg-spring behaviour. Our results also strengthen the link between repeated-sprint ability and the change in neuromuscular activation as well as in muscle de- and re-oxygenation rates.

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Abbreviations

BF:

Biceps femoris

EMG:

Electromyography

Fz max :

Maximal vertical force

K leg :

Leg stiffness

K vert :

Vertical stiffness

L 0 :

Lower-limb length

RAST:

Running anaerobic Sprint Test

RF:

Rectus femoris

RSA:

Repeated-sprint ability

RMS:

Root mean square

S dec :

Sprint decrement score

S F :

Stride frequency

S L :

Stride length

t c :

Contact time

t f :

Flight time

TSI:

Tissue saturation index

V forward :

Forward running velocity

VL:

Vastus lateralis

ΔL :

Leg compression

Δz :

Centre of mass vertical displacement

References

  1. Avela J, Komi PV (1998) Interaction between muscle stiffness and stretch reflex sensitivity after long-term stretch-shortening cycle exercise. Muscle Nerve 21:1224–1227

    Article  CAS  PubMed  Google Scholar 

  2. Balciunas M, Stonkus S, Abrantes C, Sampaio J (2006) Long term effects of different training modalities on power, speed, skill and anaerobic capacity in young male basketball players. J Sports Sci Med 5:163–170

    PubMed Central  PubMed  Google Scholar 

  3. Beck WR, Zagatto AM, Gobatto CA (2014) Repeated sprint ability tests and intensity-time curvature constant to predict short-distance running performances. Sport Sci Health 10:105–110

    Article  Google Scholar 

  4. Bishop D, Girard O, Mendez-Villanueva A (2011) Repeated-sprint ability—part II: recommendations for training. Sports Med 41:741–756

    Article  PubMed  Google Scholar 

  5. Borg G (1970) Perceived exertion as an indicator of somatic stress. Scand J Rehabil Med 2:92–98

    CAS  PubMed  Google Scholar 

  6. Brocherie F, Girard O, Faiss R, Millet GP (2014a) High-intensity intermittent training in hypoxia: a double-blinded, placebo-controlled field study in youth football players J Strength Cond Res [Epub ahead of print]

  7. Brocherie F, Girard O, Forchino F, Al Haddad H, Dos Santos G, Millet GP (2014b) Relationships between anthropometric measures and athletic performance, with special reference to repeated-sprint ability, in the Qatar national soccer team. J Sports Sci 32:1243–1254

    Article  PubMed  Google Scholar 

  8. Brughelli M, Cronin J (2008) Influence of running velocity on vertical, leg and joint stiffness: modelling and recommendations for future research. Sports Med 38:647–657

    Article  PubMed  Google Scholar 

  9. Buchheit M (2012) Fatigue during repeated sprints: precision needed. Sports Med 42:165–167

    Article  PubMed  Google Scholar 

  10. Buchheit M, Ufland P (2011) Effect of endurance training on performance and muscle reoxygenation rate during repeated-sprint running. Eur J Appl Physiol 111:293–301

    Article  PubMed  Google Scholar 

  11. Buchheit M, Cormie P, Abbiss CR, Ahmaidi S, Nosaka KK, Laursen PB (2009) Muscle deoxygenation during repeated sprint running: effect of active vs. passive recovery. Int J Sports Med 30:418–425

    Article  CAS  PubMed  Google Scholar 

  12. Buchheit M, Bishop D, Haydar B, Nakamura FY, Ahmaidi S (2010) Physiological responses to shuttle repeated-sprint running. Int J Sports Med 31:402–409

    Article  CAS  PubMed  Google Scholar 

  13. Buchheit M, Abbiss CR, Peiffer JJ, Laursen PB (2012) Performance and physiological responses during a sprint interval training session: relationships with muscle oxygenation and pulmonary oxygen uptake kinetics. Eur J Appl Physiol 112:767–779

    Article  CAS  PubMed  Google Scholar 

  14. Carling C (2013) Interpreting physical performance in professional soccer match-play: should we be more pragmatic in our approach? Sports Med 43:655–663

    Article  PubMed  Google Scholar 

  15. Cipryan L, Gajda V (2011) The influence of aerobic power on repeated anaerobic exercise in junior soccer players. J Hum Kinet 28:63–71

    Article  PubMed Central  PubMed  Google Scholar 

  16. Coleman DR, Cannavan D, Horne S, Blazevich AJ (2012) Leg stiffness in human running: comparison of estimates derived from previously published models to direct kinematic-kinetic measures. J Biomech 45:1987–1991

    Article  PubMed  Google Scholar 

  17. Delextrat A, Baliqi F, Clarke N (2013) Repeated sprint ability and stride kinematics are altered following an official match in national-level basketball players. J Sports Med Phys Fit 53:112–118

    CAS  Google Scholar 

  18. Deminice R, Rosa FT, Franco GS, Jordao AA, de Freitas EC (2013) Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition 29:1127–1132

    Article  CAS  PubMed  Google Scholar 

  19. Dupont G, McCall A, Prieur F, Millet GP, Berthoin S (2010a) Faster oxygen uptake kinetics during recovery is related to better repeated sprinting ability. Eur J Appl Physiol 110:627–634

    Article  PubMed  Google Scholar 

  20. Dupont G, Nedelec M, McCall A, McCormack D, Berthoin S, Wisloff U (2010b) Effect of 2 soccer matches in a week on physical performance and injury rate. Am J Sports Med 38:1752–1758

    Article  PubMed  Google Scholar 

  21. Faiss R, Leger B, Vesin JM, Fournier PE, Eggel Y, Deriaz O, Millet GP (2013) Significant molecular and systemic adaptations after repeated sprint training in hypoxia. PLoS ONE 8:e56522

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Farina D, Merletti R, Enoka RM (2004) The extraction of neural strategies from the surface EMG. J Appl Physiol 96:1486–1495

    Article  PubMed  Google Scholar 

  23. Faude O, Koch T, Meyer T (2012) Straight sprinting is the most frequent action in goal situations in professional football. J Sports Sci 30:625–631

    Article  PubMed  Google Scholar 

  24. Ferrari M, Muthalib M, Quaresima V (2011) The use of near-infrared spectroscopy in understanding skeletal muscle physiology: recent developments. Philos Trans A 369:4577–4590

    Article  CAS  Google Scholar 

  25. Girard O, Mendez-Villanueva A, Bishop D (2011a) Repeated-sprint ability—part I: factors contributing to fatigue. Sports Med 41:673–694

    Article  PubMed  Google Scholar 

  26. Girard O, Micallef JP, Millet GP (2011b) Changes in spring-mass model characteristics during repeated running sprints. Eur J Appl Physiol 111:125–134

    Article  PubMed  Google Scholar 

  27. Girard O, Racinais S, Kelly L, Millet GP, Brocherie F (2011c) Repeated sprinting on natural grass impairs vertical stiffness but does not alter plantar loading in soccer players. Eur J Appl Physiol 111:2547–2555

    Article  PubMed  Google Scholar 

  28. Glaister M, Howatson G, Pattison JR, McInnes G (2008) The reliability and validity of fatigue measures during multiple-sprint work: an issue revisited. J Strength Cond Res 22:1597–1601

    Article  PubMed  Google Scholar 

  29. Guex K, Millet GP (2013) Conceptual framework for strengthening exercises to prevent hamstring strains. Sports Med 43:1207–1215

    Article  PubMed  Google Scholar 

  30. Gwacham N, Wagner DR (2012) Acute effects of a caffeine-taurine energy drink on repeated sprint performance of American college football players. Int J Sport Nutr Exerc Metab 22:109–116

    CAS  PubMed  Google Scholar 

  31. Hanon C, Thepaut-Mathieu C, Vandewalle H (2005) Determination of muscular fatigue in elite runners. Eur J Appl Physiol 94:118–125

    Article  PubMed  Google Scholar 

  32. Hoffman JR, Im J, Kang J, Ratamess NA, Nioka S, Rundell KW, Kime R, Cooper J, Chance B (2005) The effect of a competitive collegiate football season on power performance and muscle oxygen recovery kinetics. J Strength Cond Res 19:509–513

    PubMed  Google Scholar 

  33. Hunter JP, Marshall RN, McNair PJ (2004) Interaction of step length and step rate during sprint running. Med Sci Sports Exerc 36:261–271

    Article  PubMed  Google Scholar 

  34. Ihsan M, Abbiss CR, Lipski M, Buchheit M, Watson G (2013) Muscle oxygenation and blood volume reliability during continuous and intermittent running. Int J Sports Med 34:637–645

    Article  CAS  PubMed  Google Scholar 

  35. Ingebrigtsen J, Dalen T, Hjelde GH, Drust B, Wisloff U (2014) Acceleration and sprint profiles of a professional elite football team in match play. Eur J Sport Sci 1–10 [Epub ahead of print]

  36. Jones B, Cooper CE (2014) Use of NIRS to assess effect of training on peripheral muscle oxygenation changes in elite rugby players performing repeated supramaximal cycling tests. Adv Exp Med Biol 812:333–339

    Article  PubMed  Google Scholar 

  37. Jonhagen S, Ericson MO, Nemeth G, Eriksson E (1996) Amplitude and timing of electromyographic activity during sprinting. Scand J Med Sci Sports 6:15–21

    Article  CAS  PubMed  Google Scholar 

  38. Kalva-Filho CA, Loures JP, Franco GS, Kaminagakura EI, Zagatto AM, Papoti M (2013) Comparison of the anaeorobic power measured by the RAST test at different footwear and surfaces conditions. Rev Bras Med Esporte 19:139–142

    Article  Google Scholar 

  39. Kaminagakura EI, Zagatto AM, Redkva PE, Gomes EB, Loures JP, Kalva-Filho CA, Papoti M (2012) Can the running-based anaerobic sprint test be used to predict anaerobic capacity? J Exerc Physiol 15:90–99

    Google Scholar 

  40. Keir DA, Theriault F, Serresse O (2013) Evaluation of the running-based anaerobic sprint test as a measure of repeated sprint ability in collegiate-level soccer players. J Strength Cond Res 27:1671–1678

    Article  PubMed  Google Scholar 

  41. Kelly LA, Racinais S, Tanner CM, Grantham J, Chalabi H (2010) Augmented low dye taping changes muscle activation patterns and plantar pressure during treadmill running. J Orthop Sports Phys Ther 40:648–655

    Article  PubMed  Google Scholar 

  42. Kime R, Karlsen T, Nioka S, Lech G, Madsen O, Saeterdal R, Im J, Chance B, Stray-Gundersen J (2003) Discrepancy between cardiorespiratory system and skeletal muscle in elite cyclists after hypoxic training. Dyn Med 2:4

    Article  PubMed Central  PubMed  Google Scholar 

  43. Koga S, Poole DC, Ferreira LF, Whipp BJ, Kondo N, Saitoh T, Ohmae E, Barstow TJ (2007) Spatial heterogeneity of quadriceps muscle deoxygenation kinetics during cycle exercise (1985). J Appl Physiol 103:2049–2056

    Article  PubMed  Google Scholar 

  44. Marshall PW, Lovell R, Jeppesen GK, Andersen K, Siegler JC (2014) Hamstring muscle fatigue and central motor output during a simulated soccer match. PLoS One 9:e102753

    Article  PubMed Central  PubMed  Google Scholar 

  45. McCully KK, Iotti S, Kendrick K, Wang Z, Posner JD, Leigh J Jr, Chance B (1994) Simultaneous in vivo measurements of HbO2 saturation and PCr kinetics after exercise in normal humans. J Appl Physiol 77:5–10

    CAS  PubMed  Google Scholar 

  46. Mendez-Villanueva A, Hamer P, Bishop D (2008) Fatigue in repeated-sprint exercise is related to muscle power factors and reduced neuromuscular activity. Eur J Appl Physiol 103:411–419

    Article  PubMed  Google Scholar 

  47. Mendez-Villanueva A, Edge J, Suriano R, Hamer P, Bishop D (2012) The recovery of repeated-sprint exercise is associated with PCr resynthesis, while muscle pH and EMG amplitude remain depressed. PLoS One 7:e51977

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  48. Mendiguchia J, Samozino P, Martinez-Ruiz E, Brughelli M, Schmikli S, Morin JB, Mendez-Villanueva A (2014) Progression of mechanical properties during on-field sprint running after returning to sports from a hamstring muscle injury in soccer players. Int J Sports Med 114(11):2281–2288

    Google Scholar 

  49. Mohr M, Krustrup P, Bangsbo J (2005) Fatigue in soccer: a brief review. J Sports Sci 23:593–599

    Article  PubMed  Google Scholar 

  50. Morin JB, Seve P (2011) Sprint running performance: comparison between treadmill and field conditions. Eur J Appl Physiol 111:1695–1703

    Article  PubMed  Google Scholar 

  51. Morin JB, Dalleau G, Kyrolainen H, Jeannin T, Belli A (2005) A simple method for measuring stiffness during running. J Appl Biomech 21:167–180

    PubMed  Google Scholar 

  52. Morin JB, Jeannin T, Chevallier B, Belli A (2006) Spring-mass model characteristics during sprint running: correlation with performance and fatigue-induced changes. Int J Sports Med 27:158–165

    Article  PubMed  Google Scholar 

  53. Morin JB, Samozino P, Zameziati K, Belli A (2007) Effects of altered stride frequency and contact time on leg-spring behavior in human running. J Biomech 40:3341–3348

    Article  CAS  PubMed  Google Scholar 

  54. Morin JB, Samozino P, Edouard P, Tomazin K (2011) Effect of fatigue on force production and force application technique during repeated sprints. J Biomech 44:2719–2723

    Article  PubMed  Google Scholar 

  55. Nagahara R, Naito H, Morin JB, Zushi K (2014) Association of acceleration with spatiotemporal variables in maximal sprinting. Int J Sports Med 35:755–761

    Article  CAS  PubMed  Google Scholar 

  56. Nagasawa T (2013) Slower recovery rate of muscle oxygenation after sprint exercise in long-distance runners compared with that in sprinters and healthy controls. J Strength Cond Res 27:3360–3366

    Article  PubMed  Google Scholar 

  57. Racinais S, Bishop D, Denis R, Lattier G, Mendez-Villaneuva A, Perrey S (2007) Muscle deoxygenation and neural drive to the muscle during repeated sprint cycling. Med Sci Sports Exerc 39:268–274

    Article  PubMed  Google Scholar 

  58. Rey E, Lago-Penas C, Lago-Ballesteros J (2012) Tensiomyography of selected lower-limb muscles in professional soccer players. J Electromyogr Kinesiol 22:866–872

    Article  PubMed  Google Scholar 

  59. Schache AG, Dorn TW, Blanch PD, Brown NA, Pandy MG (2012) Mechanics of the human hamstring muscles during sprinting. Med Sci Sports Exerc 44:647–658

    Article  PubMed  Google Scholar 

  60. Scott BR, Slattery KM, Sculley DV, Dascombe BJ (2014) Hypoxia and resistance exercise: a comparison of localized and systemic methods. Sports Med 44(8):1037–1054

    Article  PubMed  Google Scholar 

  61. Small K, McNaughton LR, Greig M, Lohkamp M, Lovell R (2009) Soccer fatigue, sprinting and hamstring injury risk. Int J Sports Med 30:573–578

    Article  CAS  PubMed  Google Scholar 

  62. Spencer M, Bishop D, Dawson B, Goodman C (2005) Physiological and metabolic responses of repeated-sprint activities: specific to field-based team sports. Sports Med 35:1025–1044

    Article  PubMed  Google Scholar 

  63. Timmins RG, Opar DA, Williams MD, Schache AG, Dear NM, Shield AJ (2014) Reduced biceps femoris myoelectrical activity influences eccentric knee flexor weakness after repeat sprint running. Scand J Med Sci Sports. doi:10.1111/sms.12171

    PubMed  Google Scholar 

  64. Winter DA (1990) Biomechanics and motor control of human movement, 2nd edn. Wiley Inter Science, New York

    Google Scholar 

  65. Wolf M, Ferrari M, Quaresima V (2007) Progress of near-infrared spectroscopy and topography for brain and muscle clinical applications. J Biomed Opt 12:062104

    Article  PubMed  Google Scholar 

  66. Zagatto AM, Beck WR, Gobatto CA (2009) Validity of the running anaerobic sprint test for assessing anaerobic power and predicting short-distance performances. J Strength Cond Res 23:1820–1827

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to the Qatar Football Association for their support and the national team players for their enthusiastic participation.

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Correspondence to Franck Brocherie.

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Communicated by Peter Krustrup.

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Brocherie, F., Millet, G.P. & Girard, O. Neuro-mechanical and metabolic adjustments to the repeated anaerobic sprint test in professional football players. Eur J Appl Physiol 115, 891–903 (2015). https://doi.org/10.1007/s00421-014-3070-z

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Keywords

  • Repeated-sprint ability
  • Spring-mass model characteristics
  • Electromyographic activity
  • Near-infrared spectroscopy
  • Football