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The effect of purinergic P2 receptor blockade on skeletal muscle exercise hyperemia in miniature swine

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Abstract

Purpose

ATP could play an important role in skeletal muscle blood flow regulation by inducing vasodilation via purinergic P2 receptors. This study investigated the role of P2 receptors in exercise hyperemia in miniature swine.

Methods

We measured regional blood flow with radiolabeled-microsphere technique and systemic hemodynamics before and after arterial infusion of the P2 receptor antagonist reactive blue 2 during treadmill exercise (5.2 km/h, ~60 % VO2max) and arterial ATP infusion in female Yucatan miniature swine (~29 kg).

Results

Mean blood flow during exercise from the 16 sampled skeletal muscle tissues was 138 ± 18 mL/min/100 g (mean ± SEM), and it was reduced in 11 (~25 %) of the 16 sampled skeletal muscles after RB2 was infused. RB2 also lowered diaphragm blood flow and kidney blood flow, whereas lung tissue blood flow was increased (all P < 0.05). Infusion of RB2 increased arterial lactate concentration during exercise from 1.6 ± 0.5 to 3.4 ± 0.6 mmol/L and heart rate from 216 ± 12 to 230 ± 9 beats/min, whereas blood pressure was unaltered. Arterial ATP infusion caused a ~twofold increase in blood flow in 15 of the 16 sampled muscle tissues and this effect was abolished after RB2 infusion.

Conclusions

These results indicate that P2 receptors play a role in regulating skeletal muscle blood flow during exercise in miniature swine.

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Abbreviations

Adr:

Adrenal

Bic Br:

Biceps brachii

Bic Fem:

Biceps femoris

Brach:

Brachialis

Dia:

Diaphragm

EDHF:

Endothelium-derived hyperpolarizing factor

Int:

Intestines

Kid:

Kidney

LV:

Left ventricle

Pan:

Pancreas

RB2:

Reactive blue 2

RF:

Rectus femoris

RV:

Right ventricle

Semit:

Semitendinosus

Stom:

Stomach

Sup:

Superficial portion

TAH:

Triceps brachii accessory head

TLH:

Triceps brachii long head

TltH:

Triceps brachii lateral head

TMH:

Triceps brachii medial head

VI:

Vastus intermedius

VL:

Vastus lateralis

VM:

Vastus medialis

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Acknowledgments

This work was supported by a grant from the Novo Nordisk foundation. The technical assistance of Cory Weimer, Dave Harah and Denise Holiman is gratefully acknowledged.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, J.B.Winsløwsvej 21-3, 5000, Odense C, Denmark

    S. P. Mortensen

  2. Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA

    R. M. McAllister, H. T. Yang & M. H. Laughlin

  3. Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark

    Y. Hellsten

Authors
  1. S. P. Mortensen
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  2. R. M. McAllister
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  3. H. T. Yang
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  4. Y. Hellsten
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  5. M. H. Laughlin
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Corresponding author

Correspondence to S. P. Mortensen.

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Communicated by Carsten Lundby.

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Mortensen, S.P., McAllister, R.M., Yang, H.T. et al. The effect of purinergic P2 receptor blockade on skeletal muscle exercise hyperemia in miniature swine. Eur J Appl Physiol 114, 2147–2155 (2014). https://doi.org/10.1007/s00421-014-2932-8

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  • DOI: https://doi.org/10.1007/s00421-014-2932-8

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