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A single bout of downhill running transiently increases HOMA-IR without altering adipokine response in healthy adult women

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Abstract

Purpose

Eccentric exercise-induced muscle damage may cause marked alterations in insulin sensitivity. However, it is not entirely known whether such alterations are also related to changes in adipokine levels. The aim of this study was to investigate the effects of muscle damage due to downhill running on inflammation, insulin sensitivity and selected adipokines related to insulin regulation (adiponectin, visfatin, resistin).

Methods

Data were collected from 12 healthy adult women. Each subject participated in two trials, 4 weeks apart. The first trial was reserved for resting measurements only (control trial), while the second trial involved a 45-min exercise (−15 % slope, ~60 % of VO2max) intervention (exercise trial). Insulin sensitivity (HOMA), creatine kinase activity (CK), delayed onset muscle soreness (DOMS), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), glucose, insulin, adiponectin, resistin, and visfatin were assessed pre-exercise and 1, 2, 3, and 4 days post-exercise and during the same time points in the control trial.

Results

Analyses revealed that CK, DOMS, TNF-α, IL-6, insulin and HOMA significantly increased (p < 0.05) throughout recovery (days 1–4). Adiponectin and visfatin remained unchanged, while resistin significantly increased (p < 0.05) only 2 days post-exercise. Visfatin was negatively correlated with HOMA at days 1 and 4 of recovery.

Conclusion

Although muscle damage due to downhill running caused a decline of insulin sensitivity, this response was not associated with the changes in adipokine levels.

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Abbreviations

AdipoR1:

Adiponectin receptors 1

AdipoR2:

Adiponectin receptors 2

BMI:

Body mass index

CK:

Creatine kinase

DOMS:

Delayed onset of muscle soreness

GLUT-4:

Glucose transporter type 4

HOMA:

Homeostasis model assessment

IL-6:

Interleukin 6

IRS-1:

Insulin receptor substrate 1

PI-3:

Phosphatidylinositol 3-kinase

PBMCs:

Peripheral blood mononuclear cells

TNF-α:

Tumor necrosis factor-α

VO2 :

Oxygen consumption

VO2max :

Maximal oxygen consumption

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Conflict of interest

The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Physical Education and Sport Science, University of Thessaly, Karies, 42100, Trikala, Greece

    A. Z. Jamurtas, A. A. Theodorou, A. Zalavras, V. Paschalis, C. K. Deli & Y. Koutedakis

  2. Institute of Human Performance and Rehabilitation, Centre for Research and Technology–Thessaly, Volos, Greece

    A. Z. Jamurtas, A. A. Theodorou, A. Zalavras, V. Paschalis, C. K. Deli, I. G. Fatouros & Y. Koutedakis

  3. Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece

    A. Garyfallopoulou

  4. Department of Physical Education and Sport Science, Aristotle University of Thessaloniki, Serres, Greece

    M. G. Nikolaidis

  5. Department of Physical Education and Sport Science, University of Thrace, Komotini, Greece

    I. G. Fatouros

  6. School of Sports, Performing Arts and Leisure, University of Wolverhampton, Wolverhampton, UK

    Y. Koutedakis

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  1. A. Z. Jamurtas
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  3. A. A. Theodorou
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  7. M. G. Nikolaidis
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  8. I. G. Fatouros
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Correspondence to A. Z. Jamurtas.

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Communicated by Jean-René Lacour.

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Jamurtas, A.Z., Garyfallopoulou, A., Theodorou, A.A. et al. A single bout of downhill running transiently increases HOMA-IR without altering adipokine response in healthy adult women. Eur J Appl Physiol 113, 2925–2932 (2013). https://doi.org/10.1007/s00421-013-2717-5

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  • DOI: https://doi.org/10.1007/s00421-013-2717-5

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