European Journal of Applied Physiology

, Volume 113, Issue 10, pp 2621–2627 | Cite as

Effects of yoga exercise on salivary beta-defensin 2

  • Nobuhiko EdaEmail author
  • Kazuhiro Shimizu
  • Satomi Suzuki
  • Yoko Tanabe
  • Eunjae Lee
  • Takao Akama
Original Article



Yoga stretching can be done comfortably and easily by beginners and older adults to compensate for lack of exercise or poor health maintenance. The aim of this study was to determine the effect of yoga stretching on mucosal immune functions, primarily human β-defensin 2 (HBD-2) in saliva.


Fifteen healthy adults (age, 60.4 ± 8.0 years) participated in the study. Participants rested for 90 min on the first day and performed yoga for 90 min on the second day. Measurements were carried out before and after rest or yoga. Saliva samples were collected by chewing a sterile cotton at a frequency of 60 cycles per min. Salivary HBD-2 concentration was measured using an enzyme-linked immunosorbent assay.


HBD-2 concentration after yoga stretching (165.4 ± 127.1 pg/mL) was significantly higher than that before yoga stretching (84.1 ± 63.4 pg/mL; p < 0.01). HBD-2 expression rate after yoga stretching (232.8 ± 192.9 pg/min) was significantly higher than that before yoga stretching (110.7 ± 96.8 pg/min; p < 0.01). HBD-2 concentration (p < 0.05) and HBD-2 expression rate (p < 0.01) at post on the second day (yoga) was significantly higher than that on the first day (rest). POMS score of anger-hostility was lower after yoga than before.


Yoga stretching for 90 min can increase salivary HBD-2 expression in older adults. Therefore, yoga stretching might be useful for older adults and athletes to maintain their health.


Stretching Relaxation Mucosal immunity Antimicrobial peptide 


  1. Ainsworth BE, Haskell WL, Whitt MC, Irwin ML, Swartz AM, Strath SJ, O’Brien WL, Bassett DR Jr, Schmitz KH, Emplaincourt PO, Jacobs DR Jr, Leon AS (2000) Compendium of physical activities: an update of activity codes and MET intensities. Med Sci Sports Exerc 32:498–504CrossRefGoogle Scholar
  2. Akimoto T, Kumai Y, Akama T, Hayashi E, Murakami H, Soma R, Kuno S, Kono I (2003) Effects of 12 months of exercise training on salivary secretory IgA levels in elderly subjects. Br J Sports Med 37:76–79PubMedCrossRefGoogle Scholar
  3. Alter MJ (2004) Science of flexibility, 3rd edn. Human Kinetics, ChampaignGoogle Scholar
  4. Diego M, Field T, Sanders C, Hernandez-Reif M (2004) Massage therapy of moderate and light pressure and vibrator effects on EEG and heart rate. Int J Neurosci 114:31–44PubMedCrossRefGoogle Scholar
  5. Dinulos JG, Mentele L, Fredericks LP, Dale BA, Darmstadt GL (2003) Keratinocyte expression of human beta defensin 2 following bacterial infection: role in cutaneous host defense. Clin Diagn Lab Immunol 10(1):161–166PubMedGoogle Scholar
  6. Field T (2011) Yoga clinical research review. Complem Ther Clin Pract 17:1–8CrossRefGoogle Scholar
  7. Field T, Diego M, Hernandez-Reif M (2007) Massage therapy research. Dev Rev 27:75–89CrossRefGoogle Scholar
  8. Ganz T (1999) Defensins and host defense. Science 286:420–421PubMedCrossRefGoogle Scholar
  9. Ganz T (2003) Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 3:710–720PubMedCrossRefGoogle Scholar
  10. Ganz T, Lehrer RI (1994) Defensins. Curr Opin Immunol 6:584–589PubMedCrossRefGoogle Scholar
  11. Greiwe JS, Hickner RC, Shah SD, Cryer PE, Holloszy JO (1999) Norepinephrine response to exercise at the same relative intensity before and after endurance exercise training. J Appl Physiol 86(2):531–535PubMedGoogle Scholar
  12. Harder J, Meyer-Hoffert U, Teran LM, Schwichtenberg L, Bartels J, Maune S, Schröder JM (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22:714–721PubMedCrossRefGoogle Scholar
  13. Hartfiel N, Havenhand J, Khalsa SB, Clarke G, Krayer A (2011) The effectiveness of yoga for the improvement of well-being and resilience to stress in the workplace. Scand J Work Environ Health 37:70–76PubMedCrossRefGoogle Scholar
  14. Houston MS, Silverstein MD, Suman VJ (1997) Risk factors for 30-day mortality in elderly patients with lower respiratory tract infection. Community-based study. Arch Intern Med 157:2190–2195PubMedCrossRefGoogle Scholar
  15. Iyengar BKS (2001) Light on yoga: the definitive guide to yoga practice. Thorsons, LondonGoogle Scholar
  16. Kamei T, Toriumi Y, Kimura H, Ohno S, Kumano H, Kimura K (2000) Decrease in serum cortisol during yoga exercise is correlated with alpha wave activation. Percept Mot Skills 90:1027–1032PubMedCrossRefGoogle Scholar
  17. Kirschbaum C, Hellhammer DH (1994) Salivary cortisol in psychoneuroendocrine research: recent developments and applications. Psychoneuroendocrinology 19:313–333PubMedCrossRefGoogle Scholar
  18. Kuboki Y, Fujisawa R (1986) Sampling and handling of saliva. J Med Technol 30:937–942Google Scholar
  19. Lamm ME, Nedrud JG, Kaetzel CS, Mazanec MB (1995) IgA and mucosal defense. APMIS 103(4):241–246PubMedCrossRefGoogle Scholar
  20. Lehrer RI, Lichtenstein AK, Ganz T (1993) Defensins: antimicrobial and cytotoxic peptides of mammalian cells. Annu Rev Immunol 11:105–128PubMedCrossRefGoogle Scholar
  21. Mataga I (2009) Dry mouth; Basic and clinical investigations upon history. Jpn J Oral Maxillofac Surg 55(4):154–162CrossRefGoogle Scholar
  22. McDermott AM, Redfern RL, Zhang B, Pei Y, Huang L, Proske RJ (2003) Defensin expression by the cornea: multiple signaling pathways mediate IL-1beta stimulation of hBD-2 expression by human corneal epithelial cells. Invest Ophthalmol Vis Sci 44:1859–1865PubMedCrossRefGoogle Scholar
  23. Oren Z, Lerman JC, Gudmundsson GH, Agerberth B, Shai Y (1999) Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis for its non-cell-selective activity. Biochem J 341:501–513PubMedCrossRefGoogle Scholar
  24. Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK (1999) Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans. J Physiol 515:287–291PubMedCrossRefGoogle Scholar
  25. Sakaki K (2006) Variation in the ratio of leukocytes in women caused by yoga breathing: The interrelationship among individual differences in the autonomic nervous system, endocrine secretion and immune mechanism activities by yoga breathing. Jpn Soc Phys Fit Sport Med 55:477–488CrossRefGoogle Scholar
  26. Schaller M, Schackert C, Korting HC, Januschke E, Hube B (2000) Invasion of Candida albicans correlates with expression of secreted aspartic proteinases during experimental infection of human epidermis. J Invest Dermatol 114:712–717PubMedCrossRefGoogle Scholar
  27. Shimizu K, Kimura F, Akimoto T, Akama T, Otsuki T, Nishijima T, Kuno S, Kono I (2007a) Effects of exercise, age and gender on salivary secretory immunoglobulin A in elderly individuals. Exerc Immunol Rev 13:55–66PubMedGoogle Scholar
  28. Shimizu K, Kimura F, Akimoto T, Akama T, Kuno S, Kono I (2007b) Effect of free-living daily physical activity on salivary secretory IgA in elderly. Med Sci Sports Exerc 39:593–598PubMedCrossRefGoogle Scholar
  29. Starner TD, Agerberth B, Gudmundsson GH, McCray PB Jr (2005) Expression and activity of beta-defensins and LL-37 in the developing human lung. J Immunol 174:1608–1615PubMedGoogle Scholar
  30. Telles S, Gaur V, Balkrishna A (2009) Effect of a yoga practice session and a yoga theory session on state anxiety. Percept Mot Skills 109:924–930PubMedCrossRefGoogle Scholar
  31. The Ministry of Health, Labour and Welfare (2006) Exercise and physical activity guide for health promotion 2006. The Ministry of Health, Labour and Welfare, TokyoGoogle Scholar
  32. Tomita T, Nagase T, Ohga E, Yamaguchi Y, Yoshizumi M, Ouchi Y (2002) Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line (LC2/ad). Respirology 7:305–310PubMedCrossRefGoogle Scholar
  33. Uebelacker LA, Tremont G, Epstein-Lubow G, Gaudiano BA, Gillette T, Kalibatseva Z, Miller IW (2010) Open trial of Vinyasa yoga for persistently depressed individuals: evidence of feasibility and acceptability. Behav Modif 34:247–264PubMedCrossRefGoogle Scholar
  34. Usui T, Yoshikawa T, Orita K, Ueda SY, Katsura Y, Fujimoto S, Yoshimura M (2011) Changes in salivary antimicrobial peptides, immunoglobulin A and cortisol after prolonged strenuous exercise. Eur J Appl Physiol 111:2005–2014PubMedCrossRefGoogle Scholar
  35. Yokoyama K, Araki S, Kawakami N, Tkakeshita T (1990) Production of the Japanese edition of profile of mood states (POMS): assessment of reliability and validity. Nihon Koshu Eisei Zasshi 37:913–918PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Nobuhiko Eda
    • 1
    Email author
  • Kazuhiro Shimizu
    • 2
  • Satomi Suzuki
    • 2
  • Yoko Tanabe
    • 1
  • Eunjae Lee
    • 1
  • Takao Akama
    • 3
  1. 1.Graduate School of Sport SciencesWaseda UniversityTokorozawaJapan
  2. 2.Sports Research and Development CoreUniversity of TsukubaTsukubaJapan
  3. 3.Faculty of Sport SciencesWaseda UniversityTokorozawaJapan

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