European Journal of Applied Physiology

, Volume 112, Issue 9, pp 3335–3340 | Cite as

Sex differences in creatine kinase after acute heavy resistance exercise on circulating granulocyte estradiol receptors

  • Megan R. Wolf
  • Maren S. Fragala
  • Jeff S. Volek
  • Craig R. Denegar
  • Jeffrey M. Anderson
  • Brett A. Comstock
  • Courtenay Dunn-Lewis
  • David R. Hooper
  • Tunde K. Szivak
  • Hui-Ying Luk
  • Carl M. Maresh
  • Keijo Häkkinen
  • William J. Kraemer
Original Article

Abstract

Previous research has shown reduced tissue disruption and inflammatory responses in women as compared to men following acute strenuous exercise. While the mechanism of this action is not known, estrogen may reduce the inflammatory response through its interaction with granulocytes. The purpose of this study was to determine if estrogen receptor β expression on granulocytes is related to sex differences in tissue disruption in response to an acute heavy resistance exercise protocol. Seven healthy, resistance-trained, eumenorrheic women (23 ± 3 years, 169 ± 9.1 cm, 66.4 ± 10.5 kg) and 8 healthy, resistance-trained men (25 ± 5 years, 178 ± 6.7 cm, 82.3 ± 9.33 kg) volunteered to participate in the study. Subjects performed an acute resistance exercise test consisting of six sets of five squats at 90% of the subject’s one repetition maximum. Blood samples were obtained pre-, mid-, post-, and 1-, 6-, and 24-h postexercise. Blood samples were analyzed for 17-β-estradiol by ELISA, creatine kinase by colorimetric enzyme immunoassay, and estradiol receptors on circulating granulocytes through flow cytometry. Men had higher CK concentrations than women at baseline/control. Men had significantly higher CK concentrations at 24-h postexercise than women. No significant changes in estradiol β receptors were expressed on granulocytes after exercise or between sexes. While sex differences occur in CK activity in response to strenuous eccentric exercise, they may not be related to estradiol receptor β expression on granulocytes. Thus, although there are sex differences in CK expression following acute resistance exercise, the differences may not be attributable to estrogen receptor β expression on granulocytes.

Keywords

Resistance exercise Tissue disruption Estrogen Skeletal muscle damage Creatine kinase 

Notes

Acknowledgments

The authors wish to thank our subjects and all volunteers who assisted in data collection.

Conflict of interest

No conflicts of interest to report.

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Megan R. Wolf
    • 1
  • Maren S. Fragala
    • 3
  • Jeff S. Volek
    • 1
  • Craig R. Denegar
    • 1
  • Jeffrey M. Anderson
    • 1
  • Brett A. Comstock
    • 1
  • Courtenay Dunn-Lewis
    • 1
  • David R. Hooper
    • 1
  • Tunde K. Szivak
    • 1
  • Hui-Ying Luk
    • 1
  • Carl M. Maresh
    • 1
    • 2
  • Keijo Häkkinen
    • 4
  • William J. Kraemer
    • 1
    • 2
  1. 1.Human Performance Laboratory, Department of KinesiologyUniversity of ConnecticutStorrsUSA
  2. 2.Department of Physiology and NeurobiologyUniversity of ConnecticutStorrsUSA
  3. 3.Sport and Exercise ScienceUniversity of Central FloridaOrlandoUSA
  4. 4.Department of Biology of Physical Activity and Neuromuscular Research CenterUniversity of JyväskyläJyväskyläFinland

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