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Modulation of circulating purines and pyrimidines by physical exercise in the horse

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Abstract

This study was designed to examine the influence of sub-maximal exercise on purine and pyrimidine catabolism in horses. Ten horses were initially trained for 12 weeks at the end of which they underwent a standardized exercise test (SET); venous blood samples were taken at rest, 5 and 30 min after the SET. Six untrained healthy horses, from which a blood withdrawal was taken at rest, were used as the control group. Samples were analyzed by HPLC for the simultaneous determination of uric acid, uridine, β-pseudouridine and creatinine in plasma. Glucose and lactate were measured in blood. Trained horses had basal uridine levels significantly lower than sedentary horses. The SET caused significant increase in plasma uric acid, uridine, β-pseudouridine and creatinine. Following the SET, a significant negative correlation was found between plasma uridine and glucose, whilst a significant positive correlation was observed between plasma uric acid and creatinine. These results indicate that increase in energy demand during exercise in the horse causes not only the degradation of purine but also of pyrimidine compounds, the latter possibly exerting a control on glucose uptake as also demonstrated in human beings.

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Acknowledgments

This work was supported in part by research funds from University of Messina (Alberghina) and University of Catania (Lazzarino). No other disclosures, nor any conflict of interest, were reported.

Conflict of interest

None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.

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Authors and Affiliations

  1. Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy

    Daniela Alberghina & Giuseppe Piccione

  2. Institute of Biochemistry and Clinical Biochemistry, Catholic University of Rome, Rome, Italy

    Angela Maria Amorini, Marika Picardi & Barbara Tavazzi

  3. Department of Chemical Sciences, Division of Biochemistry and Molecular Biology, University of Catania, Catania, Italy

    Serafina D’Urso, Salvatore Longo & Giuseppe Lazzarino

Authors
  1. Daniela Alberghina
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  2. Giuseppe Piccione
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  3. Angela Maria Amorini
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  4. Serafina D’Urso
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  5. Salvatore Longo
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  6. Marika Picardi
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  7. Barbara Tavazzi
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  8. Giuseppe Lazzarino
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Correspondence to Giuseppe Lazzarino.

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Communicated by Jacques Poortmans.

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Alberghina, D., Piccione, G., Amorini, A.M. et al. Modulation of circulating purines and pyrimidines by physical exercise in the horse. Eur J Appl Physiol 111, 549–556 (2011). https://doi.org/10.1007/s00421-010-1673-6

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  • DOI: https://doi.org/10.1007/s00421-010-1673-6

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