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The effects of eccentric contraction on myofibrillar proteins in rat skeletal muscle

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Abstract

The present study investigated the effects of eccentric muscle contractions (ECC) on the content of myofibrillar proteins (my-proteins) and the catalytic activity of myofibrillar ATPase (my-ATPase) in skeletal muscles. Rat extensor digitorum longus and tibialis anterior muscles were exposed to 200-repeated ECC or isometric contractions (ISC) and used for measures of force output and for biochemical analyses, respectively. Whereas in ISC-treated muscles, full restoration of tetanic force was attained after 2 days of recovery, force developed by ECC-treated muscles remained depressed (P < 0.05) after 6 days. The total my-protein content and the relative content of myosin heavy chain (MHC) in total my-proteins were unaltered during 4 days of recovery after ECC, but fell (P < 0.05) to 55.9 and 63.4% after 6 days of recovery, respectively. my-ATPase activity expressed on a my-protein weight basis was unaltered immediately after ECC. However, it decreased (P < 0.05) to 75.3, 45.3, and 49.3% after 2, 4 and 6 days of recovery, respectively. Total maximal calpain activity measured at 5 mM Ca2+ was significantly augmented (P < 0.05) after 2 days of recovery, reaching a level of threefold higher after 6 days. These alterations were specific for ECC and not observed for ISC. These results suggest that depressions in my-ATPase activity contribute to ECC-induced decreases in force and power which can take a number of days to recover.

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Authors and Affiliations

  1. Graduate School of Integrated Arts and Sciences, Hiroshima University, 1-7-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8521, Japan

    Keita Kanzaki, Mai Kuratani, Sachio Usui & Masanobu Wada

  2. Faculty of Human Health Science, Hachinohe University, 13-98 Mihono, Hachinohe, Aomori, 031-8588, Japan

    Takaaki Mishima

  3. Faculty of Education and Culture, Miyazaki University, 1-1 Gakuenkihanadainishi, Miyazaki, Miyazaki, 889-2192, Japan

    Satoshi Matsunaga

  4. Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashihiroshima, Hiroshima, 739-8528, Japan

    Noriyuki Yanaka

Authors
  1. Keita Kanzaki
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  2. Mai Kuratani
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  3. Takaaki Mishima
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  4. Satoshi Matsunaga
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  5. Noriyuki Yanaka
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Correspondence to Masanobu Wada.

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Communicated by Håkan Westerblad.

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Kanzaki, K., Kuratani, M., Mishima, T. et al. The effects of eccentric contraction on myofibrillar proteins in rat skeletal muscle. Eur J Appl Physiol 110, 943–952 (2010). https://doi.org/10.1007/s00421-010-1579-3

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