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Exercise-induced immune cell apoptosis: image-based model for morphological assessment

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Abstract

Methods of assessing exercise-induced lymphocyte apoptosis have produced varying results. While morphological methods generally yield a significantly greater apoptotic index compared to those employing biochemical markers, benefits and limitations are associated with each methodology. Of interest in this report is the limitation of subjectivity associated with the morphological technique. To overcome the lack of objectivity associated with the morphological method, we describe an image-based approach by which computer software assesses the characteristics associated with lymphocyte apoptosis. A stochastic controlled deformable model can be employed to detect the classic morphological apoptotic changes induced by exercise, including membrane blebbing and the formation of apoptotic bodies. We propose that an objective evaluation of multiple points in the apoptotic process through the proposed model in conjunction with current biomarker methods has the potential to advance our understanding of the exercise-induced immune cell death response to a greater degree than current methods.

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References

  • Amini A, Weymouth T, Jain R (1990) Using dynamic programming for solving variational problems in vision. IEEE Trans Pattern Anal Mach Intell 12:855–867

    Article  Google Scholar 

  • Avula CP, Muthukumar AR, Zaman K, McCarter R, Fernandes G (2001) Inhibitory effects of voluntary wheel exercise on apoptosis in splenic lymphocyte subsets of C57BL/6 mice. J Appl Physiol 91:2546–2552

    CAS  PubMed  Google Scholar 

  • Concordet J-P, Ferry A (1993) Physiological programmed cell death in thymocytes is induced by physical stress (exercise). Am J Physiol 34:C626–C629

    Google Scholar 

  • Farag A, Mohamed R, El-Baz A (2005) A unified for map estimation. IEEE Trans Geosci Remote Sens 8:855–867

    Google Scholar 

  • Ferry A, Rieu P, Le Page C, Elhabazi A, Laziri F, Rieu M (1993) Effect of physical exhaustion and glucocorticoids (dexamethasone) on T-cells of trained rats. Eur J Appl Physiol 66:455–460

    Article  CAS  Google Scholar 

  • Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DAN fragmentation. J Cell Biol 119:493–501

    Article  CAS  PubMed  Google Scholar 

  • Hansen J-B, Wilsgard L, Osterud B (1991) Biphasic changes in leukocytes induced by strenuous exercise. Eur J Appl Physiol 62:157–161

    Article  CAS  Google Scholar 

  • Hoffman-Goetz L (1999) Effect of estradiol and exercise on lymphocyte proliferation responses in female mice. Physiol Behav 68:169–174

    Article  CAS  PubMed  Google Scholar 

  • Hsu T-G, Hsu K-M, Kong C-W, Lu F-J, Cheng H, Tsai K (2002) Leukocyte mitochondria alterations after aerobic exercise in trained human subjects. Med Sci Sports Exerc 34:438–442

    Article  PubMed  Google Scholar 

  • Kass M, Witkin A, Terzopoulos D (1987) Snakes: active contour models. Int J Comput Vis 1:321–331

    Article  Google Scholar 

  • Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239–257

    CAS  PubMed  Google Scholar 

  • Kitanaka C, Kuchino Y (1996) Caspase-independent programmed cell death with necrotic morphology. Cell Death Differ 6:508–515

    Article  Google Scholar 

  • Koopman G, Reutlingsperger CP, Kuijten GA, Keehnen RM, Pasla ST, van Oers MH (1994) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415–1420

    CAS  PubMed  Google Scholar 

  • Lin Y-S, Kuo H-L, Kou C-F, Wang S-T, Yang B-C, Chen H-I (1999) Antioxidant administration inhibits exercise-induced thymocyte apoptosis in rats. Med Sci Sports Exerc 31:1594–1598

    Article  CAS  PubMed  Google Scholar 

  • Mars M, Govender S, Weston A, Naicker V, Chuturgoon A (1998) High intensity exercise: a cause of lymphocyte apoptosis? Biochem Biophys Res Commun 249:366–370

    Article  CAS  PubMed  Google Scholar 

  • Mooren FC, Blöming D, Lechtermann A, Lerch MM, Völker K (2002) Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol 93:147–153

    CAS  PubMed  Google Scholar 

  • Mooren FC, Lechtermann A, Völker K (2004) Exercise-induced apoptosis of lymphocytes depends on training status. Med Sci Sports Exerc 36:1476–1484

    Article  PubMed  Google Scholar 

  • Navalta JW, Sedlock DA, Park K-S (2005) Blood treatment influences the yield of apoptotic lymphocytes after maximal exercise. Med Sci Sports Exerc 37:369–373

    Article  PubMed  Google Scholar 

  • Navalta JW, Sedlock DA, Park K-S (2007a) Effect of exercise intensity on exercise-induced lymphocyte apoptosis. Int J Sports Med 28:539–542

    Article  CAS  PubMed  Google Scholar 

  • Navalta JW, Sedlock DA, Park K-S, McFarlin BK (2007b) Neither gender nor menstrual cycle phase influence exercise-induced lymphocyte apoptosis in untrained subjects. Appl Physiol Nutr Metab 32:481–486

    Article  PubMed  Google Scholar 

  • Navalta JW, McFarlin BK, Lyons TS, Faircloth JC, Bacon NT, Callahan ZJ (2009) Exercise-induced lymphocyte apoptosis attributable to cycle ergometer exercise in endurance trained individuals. Appl Physiol Nutr Metab 34:603–608

    Article  PubMed  Google Scholar 

  • Oshida Y, Yamanouchi K, Hayamizu S, Sato Y (1988) Effect of acute physical exercise on lymphocyte subpopulations in trained and untrained subjects. Int J Sports Med 9:137–140

    Article  CAS  PubMed  Google Scholar 

  • Paragios N, Deriche R (2000) Geodesic active contours and level sets for the detection and tracking of moving objects. IEEE Trans Pattern Anal Mach Intell 22:266–280

    Article  Google Scholar 

  • Paragios N, Mellina-Gottardo O, Armes V (2004) Gradient vector flow fast geometric active contours. IEEE Trans Pattern Anal Mach Intell 26:402–407

    Article  PubMed  Google Scholar 

  • Peters EM, Van Eden M, Tyler N, Ramautar A, Chutergoon AA (2006) Prolonged exercise does not cause lymphocyte DNA damage or increased apoptosis in well-trained endurance athletes. Eur J Appl Physiol 98:124–131

    Article  CAS  PubMed  Google Scholar 

  • Scholkopf B, Burges C, Smola A (1999) Advances in kernel methods—support vector learning. MIT Press, Cambridge

    Google Scholar 

  • Simpson RJ, Florida-James GD, Whyte GP, Black JR, Ross JA, Guy K (2007) Apoptosis does not contribute to the blood lymphocytopenia observed after intensive and downhill treadmill running in humans. Res Sports Med 15:157–174

    Article  PubMed  Google Scholar 

  • Steensberg A, Morrow J, Toft AD, Bruunsgaard H, Pedersen BK (2002) Prolonged exercise, lymphocyte apoptosis and F2-isoprostanes. Eur J Appl Physiol 87:38–42

    Article  CAS  PubMed  Google Scholar 

  • Williams DJ, Shah M (1992) A fast algorithm for active contours and curvature estimation. Proc Int Conf Comput Vis Graph Image Process 55:14–26

    Google Scholar 

  • Willingham MC (1999) Cytochemical methods for the detection of apoptosis. J Histochem Cytochem 47:1101–1109

    CAS  PubMed  Google Scholar 

  • Xu C, Prince J (1998) Snakes, shapes, and gradient vector flow. IEEE Trans Image Process 7:359–369

    Article  CAS  PubMed  Google Scholar 

  • Xu X, Gerald AL, Huang BC, Anderson DC, Payan DG, Luo Y (1998) Detection of programmed cell death using fluorescence energy transfer. Nucleic Acids Res 13:2034–2035

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Kinesiology, Recreation, and Sport, Western Kentucky University, 1906 College Heights Blvd. #11089, Bowling Green, KY, 42101-1089, USA

    James W. Navalta & T. Scott Lyons

  2. Computer Science Department, Western Kentucky University, Bowling Green, KY, USA

    Refaat Mohamed

  3. Bioengineering Department, University of Louisville, Louisville, KY, USA

    Ayman El-Baz

  4. Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX, USA

    Brian K. McFarlin

Authors
  1. James W. Navalta
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  2. Refaat Mohamed
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  3. Ayman El-Baz
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  4. Brian K. McFarlin
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  5. T. Scott Lyons
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Corresponding author

Correspondence to James W. Navalta.

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Communicated by Susan Ward.

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Navalta, J.W., Mohamed, R., El-Baz, A. et al. Exercise-induced immune cell apoptosis: image-based model for morphological assessment. Eur J Appl Physiol 110, 325–331 (2010). https://doi.org/10.1007/s00421-010-1504-9

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  • DOI: https://doi.org/10.1007/s00421-010-1504-9

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