Abstract
The aim of this study was to examine cardiovascular [heart rate variability (HRV)] and autonomic nervous system activation (by evaluating salivary alpha-amylase activity) that occur in professors both to, and after, the delivery of a lecture to 200 students and to determine whether gender is an influencing factor upon response. Fifty-two participants (26 women and 26 men) collected eight unstimulated saliva samples on 2 days (one a working day on which the lecture was given, the other a non-work or rest day). They also completed the Trait version of the State-Trait Anxiety Inventory (STAI) to assess their dispositional anxiety on the rest day and the State section of the STAI 15 min before and 10 min after their lecture, repeated at the same hour on the control (rest) day. The Perceived Stress Scale (PSS) was also recorded 15 min before the lecture. Continuous RR intervals were recorded before and after the lecture and the following HRV parameters were calculated: total spectral power (P TOT); the spectral power of the low frequency component (P LF); the high frequency component (P HF); and the ratio LF/HF. A reduction (P < 0.05) in the HF and HFnu component of HRV and an increase in the LH/HF ratio (P < 0.05) were observed at the end of the lecture. AA activity measured on the teaching day was significantly higher than that noted on the resting day. Lecturing resulted in a significant increase in the secretion of the stress marker alpha-amylase. Men and women did not differ in trait and state anxiety and no gender differences for HRV or AA activity were found.
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Bellingrath S, Weigl T, Kudielka BM (2009) Chronic work stress and exhaustion is associated with higher allostatic load in female school teachers. Stress 12:37–48
Bernardi L, Wdowczyk-Szulc J, Valentu C, Castoldi S, Paasino C, Spadacini G, Sleight P (2000) Effects of controlled breathing, mental activity and mental stress with or without verbalization on heart rate variability. J Am Coll Cardiol 35:1462–1469
Borgeat G, Chagon G, Legault Y (1984) Comparison of the salivary changes associated with a relaxing and with a stressful procedure. Psychophysiology 21:690–698
Bosch JA, Brand HS, Ligtenberg TJ, Bermond B, Hoogstraten J, Nieuw Amerongen AV (1996) Psychological stress as a determinant of protein levels and salivary-induced aggregation of Streptococcus gordonii in human saliva. Psychosom Med 58:374–382
Bosch JA, de Geus EJ, Veerman EC, Hoogstraten J, Nieuw Amerongen AV (2003) Innate secretory immunity in response to laboratory stressors that evoke distinct patterns of cardiac autonomic activity. Psychosom Med 58:374–382
Brouwers A, Tomic W (2000) A longitudinal study of teacher burnout and perceived self-efficacy in classroom management. Teach Teacher Educ 16:253–259
Carrillo E, Moya-Albiol L, Gonzalz-Bono E, Salvador A, Ricarte J, Gomez-Amor J (2001) Gender differences in cardiovascular and electrodermal responses to public speaking task: the role of anxiety and mood states. Int J Psychophysiol 42:253–264
Chandola T, Brunner E, Marmot M (2006) Chronic stress at work and the metabolic syndrome: prospective study. BMJ 4:521–525
Chatterton RT, Vogelsong KM, Lu YC, Hudgens GA (1996) Salivary alpha-amylase as a measure of endogenous adrenergic activity. Clin Physiol 16:433–448
Chatterton RT, Vogelsong KM, Lu YC, Hudgens GA (1997) Hormonal responses to psychological stress in men preparing for skydiving. J Clin Endocrinol Metab 82:2502–2509
Cohen S, Williamson G (1988) Perceived stress in a probability sample of the United States. In: Spacapam S, Oskamp S (eds) The social psychology of health: Claremont symposium on applied psychology. Sage, Newbury Park, pp 31–67
Cohen S, Kamarck T, Mermelstein R (1983) A global measure of perceived stress. J Health Soc Behav 24:385–396
Cohen H, Matar MA, Kaplan Z, Kotler M (1999) Power spectral analysis of heart rate variability in psychiatry. Psychother Psychosom 68:59–66
Cole AS, Eastoe JE (1988) Biochemistry and oral biology, 2nd edn. Wright, London, pp 476–477
Delaney JPA, Brodie DA (2000) Effects of short-term psychological stress on the time and frequency domains of heart-rate variability. Percept Mot Skills 91:515–524
Dishman RK, Nakamura Y, Garcia ME, Thompson RW, Dunn AL, Blair SN (2000) Heart rate variability, trait anxiety, and perceived stress among physically fit men and women? Int J of Psychophysiol 37:121–133
Eckberg DL (1997) Baroreflexes and the failing human heart. Circulation 96:4133–4137
Fuller BF (1992) The effects of stress-anxiety and coping styles on heart rate variability. J Psychophysiol 12:81–86
Gauthier J, Bouchard S (1993) Adaptation candienne-française de la forme révisée du State-Trait Anxiety Inventory de Spielberger. Can J Behav Sci 25:559–578
Gotlib IH, Cane DB (1989) Self-report assessment of depression and anxiety. In: Kendall PC, Watson D (eds) Anxiety and depression; distinctive and overlapping features. Academic, San Diego, pp 131–170
Granger DA, Kivlighan KT, el-Sheikh M, Gordis EB, Stroud LR (2007) Salivary alpha-amylase in biobehavioral research: recent developments and applications. Ann N Y Acad Sci 1098:122–144
Grayson J, Alvarez H (2008) School climate factors relating to teacher burnout: a mediator model. Teach Teacher Educ 24:1349–1363
Gröschl M, Rauh M (2006) Influence of commercial collection devices for saliva on the reliability of salivary steroids analysis. Steroids 71:1097–1100
Guyton AC (1991) Secretory functions of the alimentary tract. In: Guyton AC (ed) Textbook of medical physiology, 8th edn. WB Saunders, Philadelphia, p 771
Hjortskov N, Rissen D, Blangsted AK, Fallentin N, Lundberg U, Sogaard K (2004) The effect of mental stress on heart rate variability and blood pressure during computer work. Eur J Appl Physiol 92:84–89
Horsten M, Ericson M, Perski A, Wamala SP, Schenck-Gustaffson K, Orth-Gomer K (1999) Psychosocial factors and heart rate variability in healthy women. Psychosom Med 61:49–57
Houtman IL, Bakker FC (1987) Stress in student teachers during real and simulated standardized lectures. J Hum Stress 13:180–187
Houtman IL, Bakker FC (1991) Individual differences in reactivity to and coping with the stress of lecturing. J Psychosom Res 35:11–24
Iellamo F, Pizzinelli P, Massaro M, Raimondi G, Peruzzi G, Legramante JM (1999) Muscle metaboreflex contribution to sinus node regulation during static exercise: insights from spectral analysis of heart rate variability. Circulation 100:27–32
Kelly MM, Forsyth JP, Karekla M (2006) Sex differences in response to a panicogenic challenge procedure: an experimental evaluation of panic vulnerability in a non-clinical sample. Behav Res Ther 44:1421–1430
Kelly MM, Tyrka AR, Anderson GM, Price LH, Carpenter LL (2008) Sex differences in emotional and physiological responses to the Trier Social Stress Test. J Behav Ther Exp Psychiatry 39:87–98
Kirschbaum C, Kudielka BM, Gaab J, Schommer N, Hellhammer DH (1999) Impact of gender, menstrual cycle phase, and oral contraceptives on the activity of the hypothalamo-pituitary-adrenal axis. Psychosom Med 61:154–162
Kivlighan KT, Granger D (2006) Salivary alpha-amylase response to competition: relation to gender, previous experience, and attitudes. Psychoneuroendocrinology 31:703–714
Kristal-Boneh E, Raifel M, Froom P, Ribak J (1995) Heart rate variability in health and disease. Scand J Work Environ Health 21:85–95
Kudielka BM, Wüst S (2008) The cortisol awakening response (CAR): a useful tool for ambulant assessment of hypothalamus-pituitary-adrenal (HPA) axis activity. In: Columbus F (ed) Circadian rhythms and health research trends. NOVA Science Publishers Inc., New York
Kudielka BM, Buske-Kirschbaum A, Hellhammer DH, Kirschbaum C (2004) Differential heart rate reactivity and recovery after psychosocial stress (TSST) in healthy children, younger adults, and elderly adults, younger adults, and children: the impact of age and gender. Int J Behav Med 11:116–121
Ledowski T, Bein B, Hanss R, Tonner PH, Roller N, Scholz J (2005) Pseudocholinesterase activity increases and heart rate variability decreases with preoperative anxiety. Eur J Anaesthesiol 22:289–292
Lucini D, Norbiato G, Clerici M, Pagani M (2002) Hemodynamic and autonomic adjustments to real life stress conditions in humans. Hypertension 39:184–188
Lucini D, Riva S, Pizzinelli P, Pagani M (2007) Stress management at the worksite. Reversal of symptoms profile and cardiovascular dysregulation. Hypertension 49:291–297
Miu AC, Heilman RM, Miclea M (2009) Reduced heart rate variability and vagal tone in anxiety: trait versus state, and the effects of autogenic training. Auton Neurosci 145:99–103
Narita K, Murrata T, Hamada T, Takahashi T, Omori M, Suganuma N (2007) Interactions among higher trait anxiety, sympathetic activity, and endothelial function in the elderly. J Psychiatr Res 41:418–427
Nater UM, Rohleder N, Gaab J, Berger S, Jud A, Kirschbaum C, Ehlert U (2005) Human salivary alpha-amylase reactivity in a psychosocial stress paradigm. Int J Psychophysiol 55:333–342
Nater UM, La Marca R, Florin L, Moses A, Langhans W, Koller MM, Ehlert U (2006) Stress-induced changes in human salivary alpha-amylase activity—associations with adrenergic activity. Psychoneuroendocrinology 31:49–58
Nater UM, Rohleder N, Schlotz W, Ehlert U, Kirschbaum C (2007) Determinants of the diurnal course of salivary alpha-amylase. Psychoneuroendocrinology 32:392–401
Pagani M, Malliani A (2000) Interpreting spontaneous oscillations of muscle sympathetic nerve activity and of heart rate variability. J Hypertens 18:1709–1719
Pagani M, Lombardi F, Guzzetti S, Rimoldi O, Furlan R, Pizzinelli P, Sandrone G, Malfatto G, Dell’Orto S, Piccaluga E, Turiel M, Baselli G, Cerutti S, Malliani A (1986) Power spectral analysis of heart rate and arterial pressure variabilities as a marker of sympathovagal interaction in man and conscious dog. Circ Res 59:178–193
Pagani M, Montano N, Porta A, Malliani A, Abboud FM, Birkett C, Somers VK (1997) Relationship between spectral components of cardiovascular variabilities and direct measures of muscle sympathetic nerve activity in humans. Circulation 95:1441–1448
Schäffer L, Tilo Burkhardt T, Müller-Vizentini D, Rauh M, Tomaske M, Mieth AR, Bauersfeld U, Beinder E (2008) Cardiac autonomic balance in small-for-gestational-age neonates. Am J Physiol Heart Circ Physiol 294:H884–H890
Schwab KO, Heubel G, Bartels H (1992) Free epinephrine, norepinephrine and dopamine in saliva and plasma of healthy adults. Eur J Clin Chem Clin Biochem 30:541–544
Sgoifo A, Braglia F, Costoli T, Musso E, Meerlo P, Ceresini G, Troisi A (2003) Cardiac autonomic reactivity and salivary cortisol in men and women exposed to social stressors: relationship with individual ethological profile. Neurosci Biobehav Rev 27:179–188
Shinba T, Kariya N, Matsui Y, Ozawa N, Matsuda Y, Yamamoto K (2008) Decrease in heart rate variability response to task is related to anxiety and depressiveness in normal subjects. Psychiatry Clin Neurosci 62:603–609
Spielberger CD, Gorsuch RL, Lushene RE (1970) Manual for the State-Trait Anxiety Inventory. Consulting Psychologist Press, New York
Steerenberg PA, van Asperen IA, van Nieuw Amerongen A, Biewenga A, Mol D, Medema GJ (1997) Salivary levels of immunoglobulin A in triathletes. Eur J Oral Sci 105:305–309
Task Force of the European Society of Cardiology, The North American Society of Pacing and Electrophysiology (1996) Heart rate variability. Standards of measurement, physiological interpretation, and clinical use. Circulation 93:1043–1065
Turner RJ, Sugiya H (2002) Understanding salivary fluid and protein secretion. Oral Dis 8:3–11
Van Stegeren AH, Wolf O, Kindt M (2008) Salivary alpha-amylase and cortisol responses to different tasks: impact of sex. Int J Psychophysiology 69:33–40
Watkins LL, Grossman P, Krishnan R, Blumenthal JA (1999) Anxiety reduces baroreflex cardiac control in older adults with major depression. Psychosom Med 61:334–340
Weber A, Weltle D, Lederer P (2002) Zur problematik krankenheitsbedingter Frühpensionierungen von Gymnasiallehrkräften. Versicherungsmedizin 54:75–83
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Filaire, E., Portier, H., Massart, A. et al. Effect of lecturing to 200 students on heart rate variability and alpha-amylase activity. Eur J Appl Physiol 108, 1035–1043 (2010). https://doi.org/10.1007/s00421-009-1310-4
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DOI: https://doi.org/10.1007/s00421-009-1310-4