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Effect of chronic supplementation with shark liver oil on immune responses of exercise-trained rats

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Abstract

Previous studies have reported that chronic supplementation with shark liver oil (SLO) improves immune response of lymphocyte, macrophage and neutrophil in animal models and humans. In a similar manner, exercise training also stimulates the immune system. However, we are not aware of any study about the association of exercise and SLO supplementation on immune response. Thus, our main goal was to investigate the effect of chronic supplementation with SLO on immune responses of exercise-trained rats. Male Wistar rats were divided into four groups: sedentary with no supplementation (SED, n = 20), sedentary with SLO supplementation (SEDslo, n = 20), exercised (EX, n = 17) and exercised supplemented with SLO (EXslo, n = 19). Rats swam for 6 weeks, 1.5 h/day, in water at 32 ± 1°C, with a load of 6.0% body weight attached to the thorax of rat. Animals were killed 48 h after the last exercise session. SLO supplementation did not change phagocytosis, lysosomal volume, superoxide anion and hydrogen peroxide production by peritoneal macrophages and blood neutrophils. Thymus and spleen lymphocyte proliferation were significantly higher in SEDslo, EX, and EXslo groups compared with SED group (P < 0.05). Gut-associated lymphocyte proliferation, on the other hand, was similar between the four experimental groups. Our findings show that SLO and EX indeed are able to increase lymphocyte proliferation, but their association did not induce further stimulation in the adaptive immune response and also did not modify innate immunity.

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Acknowledgments

The authors would like to thank Araceli Goedert and Kleverton Krinski for their assistance during experimental trials. This study was supported by Pronex-CNPq, CAPES, and Fundação Araucária.

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Authors and Affiliations

  1. Department of Physiology, Federal University of Paraná, Biological Sciences Building, Curitiba, PR, 81530-970, Brazil

    Daniele Cristina Vitorino & Luiz Claudio Fernandes

  2. Department of Physical Education, Federal University of Paraná, Curitiba, PR, Brazil

    Cosme Franklim Buzzachera

  3. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil

    Rui Curi

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  1. Daniele Cristina Vitorino
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  2. Cosme Franklim Buzzachera
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  3. Rui Curi
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  4. Luiz Claudio Fernandes
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Correspondence to Luiz Claudio Fernandes.

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Communicated by William Kraemer.

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Vitorino, D.C., Buzzachera, C.F., Curi, R. et al. Effect of chronic supplementation with shark liver oil on immune responses of exercise-trained rats. Eur J Appl Physiol 108, 1225–1232 (2010). https://doi.org/10.1007/s00421-009-1267-3

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