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Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise

Abstract

We analysed the effects of resistance exercise upon the phosphorylation state of proteins associated with adaptive processes from the Akt/PKB (protein kinase B) and the mitogen-activated protein kinase (MAPK) pathways. Nine healthy young men (21.7 ± 0.55 year) performed 10 sets of 10 leg extensions at 80% of their 1-RM (repetition maximum). Muscle biopsies were taken from the vastus lateralis at rest, within the first 30 s after exercise and at 24 h post-exercise. Immediately post exercise, the phosphorylation states of Akt/PKB on Thr308 and Ser473 and 4E-BP1 on Thr37/46 (eukaryotic initiation factor 4E-binding protein 1) were decreased (−60 to −90%, P < 0.05). Conversely, the phosphorylation of p70s6k (p70 ribosomal S6 kinase) on Thr421/Ser424 was increased more than 20-fold (P < 0.05), and this was associated with a 10- to 50-fold increase in the phosphorylation of p38 and ERK1/2 (extracellular signal-regulated kinase) (P < 0.05). Twenty-four hours post-exercise the phosphorylation state of Akt/PKB on Thr308 was depressed, whereas the phosphorylation of p70s6k on Thr421/Ser424 and sarcoplasmic ERK1/2 were elevated. The present results indicate that high-intensity resistance exercise in the fasted state inhibits Akt/PKB and 4E-BP1 whilst concomitantly augmenting MAPK signalling and p70s6k on Thr421/Ser424.

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Acknowledgments

This work was supported by grants to MJ Rennie from UK Biotechnology and Biological Sciences Research Council (BB/X510697/1 and BB/C516779/1), US National Institute of Health AR 49869, and the EC EXEGENESIS program and to M Francaux from the Fonds de la Recherche Scientifique Medicale (3.4574.03).

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Correspondence to Marc Francaux.

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Deldicque, L., Atherton, P., Patel, R. et al. Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise. Eur J Appl Physiol 104, 57–65 (2008). https://doi.org/10.1007/s00421-008-0786-7

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  • DOI: https://doi.org/10.1007/s00421-008-0786-7

Keywords

  • Cell Signaling
  • Protein synthesis
  • Resistance exercise
  • MAPK
  • p70s6k