European Journal of Applied Physiology

, Volume 103, Issue 3, pp 323–332 | Cite as

Effects of concentric and repeated eccentric exercise on muscle damage and calpain–calpastatin gene expression in human skeletal muscle

  • Kristian Vissing
  • Kristian Overgaard
  • Anders Nedergaard
  • Anne Fredsted
  • Peter Schjerling
Original Article

Abstract

The purpose of this study was to compare the responsiveness of changes in Ca2+-content and calpain–calpastatin gene expression to concentric and eccentric single-bout and repeated exercise. An exercise group (n = 14) performed two bouts of bench-stepping exercise with 8 weeks between exercise bouts, and was compared to a control-group (n = 6). Muscle strength and soreness and plasma creatine kinase and myoglobin were measured before and during 7 days following exercise bouts. Muscle biopsies were collected from m. vastus lateralis of both legs prior to and at 3, 24 h and 7 days after exercise and quantified for muscle Ca2+-content and mRNA levels for calpain isoforms and calpastatin. Exercise reduced muscle strength and increased muscle soreness predominantly in the eccentric leg (P < 0.05). These responses as well as plasma levels of creatine kinase and myoglobin were all attenuated after the repeated eccentric exercise bout (P < 0.05). Total muscle Ca2+-content did not differ between interventions. mRNA levels for calpain 2 and calpastatin were upregulated exclusively by eccentric exercise 24 h post-exercise (P < 0.05), with no alteration in expression between bouts. Calpain 1 and calpain 3 mRNA did not change at any specific time point post-exercise for either intervention. Our mRNA results suggest a regulation on the calpain–calpastatin expression response to muscle damaging eccentric exercise, but not concentric exercise. Although a repeated bout effect was demonstrated in terms of muscle function, no immediate support was provided to suggest that regulation of expression of specific system components is involved in the repeated bout adaptation.

Keywords

Calcium proteolysis Repeated bout effect Single-bout Transcriptional regulation 

Supplementary material

421_2008_709_MOESM1_ESM.ppt (525 kb)
ESM1 (PPT 525 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Kristian Vissing
    • 1
  • Kristian Overgaard
    • 1
  • Anders Nedergaard
    • 2
    • 4
  • Anne Fredsted
    • 3
  • Peter Schjerling
    • 4
    • 5
  1. 1.Department of Sport ScienceUniversity of AarhusAarhus CDenmark
  2. 2.Institute of Sports MedicineBispebjerg HospitalCopenhagenDenmark
  3. 3.Institute of Physiology and BiophysicsUniversity of AarhusAarhusDenmark
  4. 4.Department of Molecular Muscle BiologyCopenhagen Muscle Research CentreCopenhagenDenmark
  5. 5.Department of Medical Biochemistry and GeneticsUniversity of CopenhagenCopenhagenDenmark

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