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European Journal of Applied Physiology

, Volume 101, Issue 5, pp 533–537 | Cite as

The effect of whole-body cryostimulation on the prooxidant–antioxidant balance in blood of elite kayakers after training

  • Alina Wozniak
  • Bartosz Wozniak
  • Gerard Drewa
  • Celestyna Mila-Kierzenkowska
Original Article

Abstract

The effect of whole-body cryostimulation prior to kayak training on the prooxidant-antioxidant balance was evaluated and compared to the effect of a single cryostimulation treatment in untrained men. The kayakers underwent a ten-day training cycle with pre-training daily whole-body cryostimulation for three min (temperature: –120 to –140°C) and training without cryostimulation as a control. Blood samples were obtained before and after the sixth and the tenth day of training and from the untrained men before and 20 min after cryostimulation. In untrained men cryostimulation induced an increase in the activity of superoxide dismutase (SOD) by 36% (P < 0.001) and glutathione peroxidase (GPx) by 68% (P < 0.01) in the erythrocytes and an increase in the conjugated dienes (CD) in plasma by 36% (P < 0.05) and in the erythrocytes by 71% (P < 0.001). In the kayakers comparing both types of training after the sixth day, the level of CD in plasma was 46 (P < 0.001) and 40% (P < 0.01) lower in erythrocytes, and the concentration of thiobarbituric acid-reactive substances in plasma was 24% (P < 0.05) lower with pre-training cryostimulation. After the sixth day of training with cryostimulation, SOD activity was also 47% (P < 0.001) lower, while GPx activity after the tenth day was reduced by more than 50% (P < 0.01) as compared to control training. Whole-body cryostimulation per se stimulates the generation of reactive oxygen species. Yet, the oxidative stress induced by kayak training was reduced by prior exposure to extremely low temperatures.

Keywords

Sport Cryo-chamber Lipid peroxidation Antioxidant enzymes 

References

  1. Alessio HM (1993) Exercise-induced oxidative stress. Med Sci Sports Exerc 25:218–224PubMedGoogle Scholar
  2. Biały D, Zimmer K, Zagrobelny Z (1999) Whole-body cryotherapy in sport. Med Sport 15:21–24Google Scholar
  3. Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140PubMedGoogle Scholar
  4. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. In: Fleisher S, Packer I (eds) Methods in enzymology. Academic, New York, pp 302–310Google Scholar
  5. Dugue B, Smolander J, Westerlund T, Oksa J, Nieminen R, Moilanen E, Mikkelsson M (2005) Acute and long-term effects of winter swimming and whole-body cryotherapy on plasma antioxidative capacity in healthy women. Scand J Clin Lab Invest 65:395–402PubMedCrossRefGoogle Scholar
  6. Janiszewski M (1998) Cryotherapy as a factor supporting rehabilitation. Med Sportiva 2:257–260Google Scholar
  7. Jenkins RR (1988) Free radical chemistry. Relationship to exercise. Sports Med 5:156–170PubMedGoogle Scholar
  8. Kopprasch S, Gatzweiler A, Gressler J, Schroder HE (1997) Beta-adrenergic modulation of FMLP-and zymosan–induced intracellular and extracellular production by polymorphonuclear leukocytes. Mol Cell Biochem 168:133–139PubMedCrossRefGoogle Scholar
  9. Kruk A, Kładna A (1998) The effect of exercise training on metabolism of catecholamines. Med Sport 14:9–16Google Scholar
  10. Metzger D, Zwingmann C, Protz W, Jackel WH (2000) Whole-body cryotherapy in rehabilitation of patients with rheumatoid diseases: pilot study. Rehabilitation (Stuttg) 39:93–100Google Scholar
  11. Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175PubMedGoogle Scholar
  12. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutatione peroxidase. J Lab Clin Med 70:158–169PubMedGoogle Scholar
  13. Sergent O, Morel I, Cogrel P, Chevanne M, Pasdeloup N, Brissot P, Lescoat G, Cillard P, Cillard J (1993) Simultaneous measurements of conjugated dienes and free malondialdehyde, used as a micromethod for the evaluation of lipid peroxidation in rat hepatocyte cultures. Chem Phys Lipids 65:133–139PubMedCrossRefGoogle Scholar
  14. Urso ML, Clarkson PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189:41–54PubMedCrossRefGoogle Scholar
  15. Wozniak A, Drewa G, Wozniak B, Drewa T, Olszewska D, Mila-Kierzenkowska C, Rakowski A, Brzuchalski M (2002) Effect of cryogenic temperatures and exercise on antioxidant enzymes activity in erythrocytes of kayakers. Biol Sport 19:63–72Google Scholar
  16. Wozniak A, Wozniak B, Drewa G, Mila-Kierzenkowska C, Rakowski A (2007) The effect of whole-body cryostimulation on lysosomal enzyme activity in kayakers during training. Eur J Appl Physiol 100:137–142PubMedCrossRefGoogle Scholar
  17. Zagrobelny Z, Halawa B, Jezierski C, Wawrowska A (1993) Effect of a single cooling of the entire body in the cryogenic chamber on selected hemodynamic parameters and blood serum hormone levels in healthy subjects. Pol Tyg Lek 48:303–305PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Alina Wozniak
    • 1
  • Bartosz Wozniak
    • 2
  • Gerard Drewa
    • 1
  • Celestyna Mila-Kierzenkowska
    • 1
  1. 1.The Chair of Medical Biology, Collegium MedicumNicolaus Copernicus UniversityBydgoszczPoland
  2. 2.Department and Clinic of Neurosurgery and Neurotraumatology, Collegium MedicumNicolaus Copernicus UniversityBydgoszczPoland

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