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Effects of two types of inactivity on the number of white blood cells in rats

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Abstract

Prolonged inactivity is known to induce changes in responses of many physiological defense systems such as the hypothalamo-hypophyseal-adrenocortical axis, the sympathetic nervous system, and immuno-responsive systems. However, effects of various types of inactivity on immuno-responsive systems are still unknown. Therefore, the effects of two types of inactivity (immobilization: IMM and whole body suspension: WBS) on the number of white blood cells were studied in rats. Rats were divided into the control group and each inactivity group to compare the number of total white blood cells, lymphocytes, monocyte, neutrophil, eosinophil, and basophil during the experimental periods. Both IMM and WBS were maintained for 11 days. IMM markedly increased the number of total white blood cells, monocyte, neutrophil, and eosinophil in the 1st to 10th day. However, the number of total white blood cells, monocyte, neutrophil, and eosinophil during the experiment of WBS were characterized by the presence of a lag phase followed by the significant increased actions. IMM did not change the number of basophil during the experimental period. However, WBS increased the number of basophil in the 1st to 8th day to 2.8–4.8 times, compared with the values of the control. Both IMM and WBS did not change the number of lymphocytes. From these results, WBS increases the number of natural immunity cells without changing acquired immunity cells, and there are different responses in the number of total white blood cells, monocyte, neutrophil, eosinophil, and basophil between IMM and WBS.

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Acknowledgments

We are grateful to Dr. Takashi Ichinose, Dr. Sachiko Nomura, Ms. Yuko Suzuki, Yuriko Higashino, Takashi Wada, and Yu Kawashima (Faculty of Human Sciences, Waseda University) for their good cooperation and helpful supports. Thanks are also due to Dr. Katsuhiko Suzuki for his advice and suggestions. This study was supported by a Grant-in-Aid for the Academic Frontier Project of the Ministry of Education, Culture, Sports, Science and Technology.

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Authors and Affiliations

  1. Laboratory of Physiological Sciences, Faculty of Human Sciences, Waseda University, 2-579-15 Mikajima, Tokorozawa, 359-1192, Japan

    Ken Shirato, Norio Motohashi, Jun Tanihata & Kazuhiko Imaizumi

  2. Department of Living and Health Sciences, Joetsu University of Education, Yamayashiki, Joetsu, 943-8512, Japan

    Kaoru Tachiyashiki

  3. Department of Biochemistry, Tokyo Medical University, 6-1-1, Shinjuku, Tokyo, 160-8402, Japan

    Akio Tomoda

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  1. Ken Shirato
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  2. Norio Motohashi
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  3. Jun Tanihata
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  4. Kaoru Tachiyashiki
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  6. Kazuhiko Imaizumi
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Correspondence to Kazuhiko Imaizumi.

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Shirato, K., Motohashi, N., Tanihata, J. et al. Effects of two types of inactivity on the number of white blood cells in rats. Eur J Appl Physiol 98, 590–600 (2006). https://doi.org/10.1007/s00421-006-0306-6

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