European Journal of Applied Physiology

, Volume 97, Issue 1, pp 109–121 | Cite as

The effects of intensive, moderate and downhill treadmill running on human blood lymphocytes expressing the adhesion/activation molecules CD54 (ICAM-1), CD18 (β2 integrin) and CD53

  • Richard J. Simpson
  • Geraint D. Florida-James
  • Greg P. Whyte
  • Keith Guy
Original Article

Abstract

This study examined the effects of intensive, moderate and downhill treadmill running on blood lymphocyte expression of adhesion/activation (AA) molecules. Trained subjects completed three treadmill-running protocols of identical duration: (1) an intensive protocol at 80% \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} \) to volitional exhaustion, (2) a moderate protocol at 60% \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} \) and (3) a −10% downhill (eccentric) protocol at 80% \( \ifmmode\expandafter\dot\else\expandafter\.\fi{V}_{{{\text{O}}_{{2\max }} }} \). Blood samples were taken before, immediately after, 1 and 24 h after exercise. Isolated lymphocytes were assessed for expression of the AA molecules CD54, CD18 and CD53 by flow cytometry. Lymphocyte counts increased immediately after all running protocols. Lymphocytopenia was observed 1 h after the intensive and eccentric protocols only. Plasma creatine kinase increased 24 h after the downhill protocol only. Increases in the number and percentage of CD54+, CD18bright and CD53bright lymphocytes were observed immediately after the intensive and eccentric protocols, with the numbers falling below pre-exercise values at 1 h post-exercise for all protocols. No differences were found between the intensive protocol and the eccentric protocol at the same relative intensity. Analysis of lymphocyte subsets showed that the total number of CD3+, CD4+, CD8+ and CD56+ lymphocytes increased after the intensive protocol before falling below pre-exercise values at 1 h post-exercise. A relatively greater mobilisation of CD56+ and CD8+ cells accounts for the changes in CD54+, CD18bright and CD53bright cell populations. Lymphocytes that enter and exit the circulation following exercise express high levels of AA molecules, which may mediate extravasation and post-exercise lymphocytopenia. This effect appears to be influenced by exercise intensity and not muscle damage.

Keywords

Lymphocytosis Lymphocytopenia CD antigens Muscle damage Flow cytometry 

References

  1. Cao L, Yoshino T, Kawasaki N, Sakuma I, Takahashi K, Akagi T (1997) Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation. Immunobiology 197:70–81PubMedGoogle Scholar
  2. Gabriel HH, Kindermann W (1998) Adhesion molecules during immune response to exercise. Can J Physiol Pharmacol 76:512–523PubMedCrossRefGoogle Scholar
  3. Gannon GA, Rhind SG, Shek PN, Shephard RJ (2001) Differential cell adhesion molecule expression and lymphocyte mobilisation during prolonged aerobic exercise. Eur J Appl Physiol 84:272–282PubMedCrossRefGoogle Scholar
  4. Goebel MU, Mills PJ (2000) Acute psychological stress and exercise and changes in peripheral leukocyte adhesion molecule expression and density. Psychosom Med 62:664–670PubMedGoogle Scholar
  5. Guy K, Crichton DN, Ross JA (1988) Indirect immunofluorescence labelling with complexes of phycoerythrin and monoclonal anti-phycoerythrin antibodies (PEAPE complexes). J Immunol Methods 112:261–265PubMedCrossRefGoogle Scholar
  6. Hay JB, Andrade WN (1998) Lymphocyte recirculation, exercise, and immune responses. Can J Physiol Pharmacol 76:490–496PubMedCrossRefGoogle Scholar
  7. Hong S, Johnson TA, Farag NH, Guy HJ, Matthews SC, Ziegler MG, Mills PJ (2005) Attenuation of T lymphocyte demargination and adhesion molecule expression in response to moderate exercise in physically fit individuals. J Appl Physiol 98:1057–1063PubMedCrossRefGoogle Scholar
  8. Hubbard AK, Rothlein R (2000) Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic Biol Med 28:1379–1386PubMedCrossRefGoogle Scholar
  9. Jalkanen S, Salmi M (1999) Lymphocytes: recirculation. In: Encyclopedia of life sciences (September). Wiley, Chichester http://www.els.net/
  10. Jilma B, Eichler HG, Stohlawetz P, Dirnberger E, Kapiotis S, Wagner OF, Schutz W, Krejcy K (1997) Effects of exercise on circulating vascular adhesion molecules in healthy men. Immunobiology 197:505–512PubMedGoogle Scholar
  11. Jordan J, Beneke R, Hutler M, Veith A, Haller H, Luft FC (1997) Moderate exercise leads to decreased expression of beta1 and beta2 integrins on leucocytes. Eur J Appl Physiol Occup Physiol 76:192–194PubMedCrossRefGoogle Scholar
  12. Jordan J, Beneke R, Hutler M, Veith A, Luft FC, Haller H (1999) Regulation of MAC-1 (CD11b/CD18) expression on circulating granulocytes in endurance runners. Med Sci Sports Exerc 31:362–367PubMedCrossRefGoogle Scholar
  13. Kendall A, Hoffman-Goetz L, Houston M, MacNeil B, Arumugam Y (1990) Exercise and blood lymphocyte subset responses: intensity, duration, and subject fitness effects. J Appl Physiol 69:251–260PubMedGoogle Scholar
  14. Kuhlwein EC, Irwin MR, Ziegler MG, Woods VL, Kennedy B, Mills PJ (2001) Propranolol affects stress-induced leukocytosis and cellular adhesion molecule expression. Eur J Appl Physiol 86:135–141PubMedCrossRefGoogle Scholar
  15. Kurokawa Y, Shinkai S, Torii J, Hino S, Shek PN (1995) Exercise-induced changes in the expression of surface adhesion molecules on circulating granulocytes and lymphocytes subpopulations. Eur J Appl Physiol Occup Physiol 71:245–252PubMedCrossRefGoogle Scholar
  16. Lee SH, Corry DB (2004) Homing alone? CD18 in infectious and allergic disease. Trends Mol Med 10:258–262PubMedCrossRefGoogle Scholar
  17. Malm C, Nyberg P, Engstrom M, Sjodin B, Lenkei R, Ekblom B, Lundberg I (2000) Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies. J Physiol 529(Pt 1):243–262PubMedCrossRefGoogle Scholar
  18. Mars M, Govender S, Weston A, Naicker V, Chuturgoon A (1998) High intensity exercise: a cause of lymphocyte apoptosis? Biochem Biophys Res Commun 249:366–370PubMedCrossRefGoogle Scholar
  19. Mooren FC, Bloming D, Lechtermann A, Lerch MM, Volker K (2002) Lymphocyte apoptosis after exhaustive and moderate exercise. J Appl Physiol 93:147–153PubMedGoogle Scholar
  20. Nielsen HG, Lyberg T (2004) Long-distance running modulates the expression of leucocyte and endothelial adhesion molecules. Scand J Immunol 60:356–362PubMedCrossRefGoogle Scholar
  21. Nieman DC (1994) Exercise, upper respiratory tract infection, and the immune system. Med Sci Sports Exerc 26:128–139PubMedCrossRefGoogle Scholar
  22. Nieman DC, Simandle S, Henson DA, Warren BJ, Suttles J, Davis JM, Buckley KS, Ahle JC, Butterworth DE, Fagoaga OR et al (1995) Lymphocyte proliferative response to 2.5 h of running. Int J Sports Med 16:404–409PubMedCrossRefGoogle Scholar
  23. Pedersen BK, Ullum H (1994) NK cell response to physical activity: possible mechanisms of action. Med Sci Sports Exerc 26:140–146PubMedCrossRefGoogle Scholar
  24. Perez CJ, Nemet D, Mills PJ, Scheet TP, Ziegler MG, Cooper DM (2001) Effects of laboratory versus field exercise on leukocyte subsets and cell adhesion molecule expression in children. Eur J Appl Physiol 86:34–39PubMedCrossRefGoogle Scholar
  25. Peters EM (1997) Exercise, immunology and upper respiratory tract infections. Int J Sports Med 18(Suppl 1):S69–S77PubMedCrossRefGoogle Scholar
  26. Pizza FX, Mitchell JB, Davis BH, Starling RD, Holtz RW, Bigelow N (1995) Exercise-induced muscle damage: effect on circulating leukocyte and lymphocyte subsets. Med Sci Sports Exerc 27:363–370PubMedGoogle Scholar
  27. Rehman J, Mills PJ, Carter SM, Chou J, Thomas J, Maisel AS (1997) Dynamic exercise leads to an increase in circulating ICAM-1: further evidence for adrenergic modulation of cell adhesion. Brain Behav Immun 11:343–351PubMedCrossRefGoogle Scholar
  28. Schwane JA, Johnson SR, Vandenakker CB, Armstrong RB (1983) Delayed-onset muscular soreness and plasma CPK and LDH activities after downhill running. Med Sci Sports Exerc 15:51–56PubMedGoogle Scholar
  29. Shephard RJ (2003) Adhesion molecules, catecholamines and leucocyte redistribution during and following exercise. Sports Med 33:261–284PubMedCrossRefGoogle Scholar
  30. Smith LL, Bond JA, Holbert D, Houmard JA, Israel RG, McCammon MR, Smith SS (1998) Differential white cell count after two bouts of downhill running. Int J Sports Med 19:432–437PubMedCrossRefGoogle Scholar
  31. Steensberg A, Morrow J, Toft AD, Bruunsgaard H, Pedersen BK (2002) Prolonged exercise, lymphocyte apoptosis and F2-isoprostanes. Eur J Appl Physiol 87:38–42PubMedCrossRefGoogle Scholar
  32. van Eeden SF, Granton J, Hards JM, Moore B, Hogg JC (1999) Expression of the cell adhesion molecules on leukocytes that demarginate during acute maximal exercise. J Appl Physiol 86:970–976PubMedGoogle Scholar
  33. Vestweber D, Blanks JE (1999) Mechanisms that regulate the function of the selectins and their ligands. Physiol Rev 79:181–213PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Richard J. Simpson
    • 1
  • Geraint D. Florida-James
    • 1
  • Greg P. Whyte
    • 2
  • Keith Guy
    • 1
  1. 1.Biomedicine and Sport and Exercise Science Research Group, School of Life SciencesNapier UniversityEdinburghUK
  2. 2.The Performance CentreEnglish Institute of SportMarlowUK

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