Abstract
Free tryptophan (Trp), which is augmented by liberated free fatty acids (FFA) from adipose tissue, can induce mental fatigue via serotonin during exercise. Since an attenuation in FFA has been observed with omega-3 fatty acid (n-3fa) use, our purpose was to examine the effect of n-3fa supplementation on free Trp availability and exercise fatigue. Ten recreationally trained men (n=5) and women (n=5), with maximal oxygen consumption (V̇O2max)of 51.6 (3.0) and 44.3 (1.4) ml kg−1 min−1, respectively, were studied on two occasions following an overnight fast, before and after n-3fa supplementation (4 g day−1 for 4 weeks). The exercise trials consisted of a 75-min treadmill run at 60% V̇O2max followed immediately by a high-intensity incremental bout to fatigue. Measurements included exercise monitors, plasma volume (PV), triglycerides (TG), FFA, glycerol, lactate, and glucose. Free Trp and branched-chain amino acids (BCAA) were measured and correlated with time to fatigue; all blood variables were corrected for PV. Free Trp, lactate, glucose, FFA, and glycerol were not significantly different between trials, but TG (P<0.001) and the free Trp/BCAA ratio were significantly lower after n-3fa use [1.76 (0.18)×10-2 μg ml−1] versus before supplementation [2.17 (0.22), P=0.033]. There was a non-significant increase in time to fatigue after supplementation [10.2 (0.3) min] versus before n-3fa use [9.7 (0.2), P=0.068], and a tendency for higher BCAA levels after supplementation, P=0.068. However, neither free Trp nor the free Trp/BCAA ratio significantly predicted time to fatigue. In conclusion, n-3fa supplementation did not diminish free Trp concentrations or significantly improve endurance performance during a maximal bout of exercise.
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References
ACSM (2000) ACSM’s guidelines for exercise testing and prescription. Lippincott, Williams & Wilkins, Baltimore, Md.
Ayre AJ, Hulbert AJ. (1997) Dietary fatty acid profile affects endurance in rats. Lipids 32:1265–1270
Bailey SP, Davis JM, Ahlborn EN (1993) Serotonergic agonists and antagonists affect endurance performance in the rat. Int J Sports Med 14:330–333
Blomstrand EP, Hassmen P, Ekblom B, Newsholme EA (1991) Administration of branched-chain amino acids during sustained exercise-effects on performance and on plasma concentration of some amino acids. Eur J Appl Physiol 63:83–88
Blomstrand EP, Hassmen P, EKS, Ekblom B, Newsholme EA (1997) Influence of ingesting a solution of branched-chain amino acids on perceived exertion during exercise. Acta Physiol Scand 159:41–49
Calders P, Pannier JL, Matthys DM, Lacroix EM (1997) Pre-exercise branched-chain amino acid administration increases endurance performance in rats. Med Sci Sports Exerc 29:1182–1186
Chaouloff F, Kennett GA, Serrurier B, Merina D, Curson G (1986) Amino acid analysis demonstrates that increased plasma free tryptophan causes the increase of brain tryptophan during exercise in the rat. J Neurochem 46:1647–1650
Clarke SD (2001) Nonalcoholic steatosis and steatohepatitis. I. Molecular mechanism for polyunsaturated fatty acid regulation of gene transcription. Am J Physiol Gastrointest Liver Physiol 281:G865–G869
Dagnelie PC, Rietveld T, Swart GR, Stijnen T, van den Berg JWO (1994) Effect of dietary fish oil on blood levels of free fatty acids, ketone bodies and triacylglycerol in humans. Lipids 29:41–45
Davis JM (1999) Effects of branched-chain amino acids and carbohydrate on fatigue during intermittent, high intensity running. Int J Sports Med 20:309–314
Davis JM, Bailey SP, Woods JA, Galiano FJ, Hamilton M, Bartoli WP (1992) Effects of carbohydrate feedings on plasma free-tryptophan and branched chain amino acids during prolonged cycling. Eur J Appl Physiol 65:513–519
Davis JM, Alderson NL, Welch RS (2000) Serotonin and central nervous system fatigue: nutritional considerations. Am J Clin Nutr 72:573S–578S
Diksic M, Young SN (2001) Study of the brain serotonergic system with labeled alpha-methyl-L-tryptophan. J Neurochem 78:1185–1200
Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248
Harris WS, Muzio F (1993) Fish oil reduces postprandial triglyceride concentrations without accelerating lipid-emulsion removal rates. Am J Clin Nutr 58:68–74
Hijikata Y, Hara S, Shiozaki KY, Murata K, Sameshima Y (1984) Determination of free tryptophan in plasma and its clinical applications. J Clin Chem Clin Biochem 22:291–299
Muoio DM, Leddy JJ, Horvath PJ, Awad AB, Pendergast DR (1994) Effect of dietary fat on metabolic adjustments to maximal VO2 and endurance in runners. Med Sci Sports Exerc 26:81–88
Rennie MJ, Tipton KD (2000) Protein and amino acid metabolism during and after exercise and the effects of nutrition. Annu Rev Nutr 20:457–483
Singer P, Wirth M, Berger I (1990) A possible contribution of decrease in free fatty acids to low serum triglyceride levels after diets supplemented with n-6 and n-3 polyunsaturated fatty acids. Atherosclerosis 83:167–175
Siri WE (1956) Body composition from fluid spaces and density. Donner Laboratory of Medical Physics, University of California Report, Berkeley, Calif., 19 March
Thomas TR, Etheridge GL (1980) Hydrostatic weighing at residual volume and functional residual capacity. J Appl Physiol 49:157–159
Thomas TR, Fisher BA, Kist WB, Horner KE, Cox RH (2000) Effects of exercise and n-3 fatty acids on postprandial lipemia. J Appl Physiol 88:2199–2204
Walker V, Mills GA (1995) Quantitative method for amino acid analysis in biological fluids. Ann Clin Biochem 32:28–57
Walsh ML (2000) Whole body fatigue and critical power: a physiological interpretation. Sports Med 29:153–166
Wilson WM, Maughan RJ (1992) Evidence for a possible role of 5-hydroxytryptamine in the genesis of fatigue in man: administration of paroxetine, a 5-HT re-uptake inhibitor, reduces the capacity to perform prolonged exercise. Exp Physiol 77:921–924
Wong SH, Marsh JB (1988) Inhibition of apolipoprotein secretion and phophatidate phosphohydrolase activity by eicosapentaenoic and docosahexaenoic acids in the perfused rat liver. Metabolism 37:1177–1181
Zak A, Zeman M, Tvrzicka E, Stolba P (1996) The effect of fish oil on metabolic parameters in patients with type 2 diabetes mellitus. Cas Lek Cesk 135:354–359
Zanker CL, Swaine IL, Castell LM, Newsholme EA (1997) Responses of plasma glutamine, free tryptophan and branched-chain amino acids to prolonged exercise after a regime designed to reduce muscle glycogen. Eur J Appl Physiol 75:543–548
Zhang ZQ, Thomas TR, Ball SD (1998) Effect of exercise timing on postprandial lipemia and HDL cholesterol subfractions. J Appl Physiol 85:1516–1522
Acknowledgements
We thank Dr. Bryan Smith for his assistance with experiments and each of the subjects for their willing participation. This study was funded in part by the University of Missouri Alumni Association and the Elizabeth Hegarty Foundation.
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Huffman, D.M., Altena, T.S., Mawhinney, T.P. et al. Effect of n-3 fatty acids on free tryptophan and exercise fatigue. Eur J Appl Physiol 92, 584–591 (2004). https://doi.org/10.1007/s00421-004-1069-6
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DOI: https://doi.org/10.1007/s00421-004-1069-6