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Prolonged vitamin C supplementation and recovery from eccentric exercise

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Abstract

We have previously shown that vitamin C supplementation affects recovery from an unaccustomed bout of demanding exercise, with the most pronounced effect being that on plasma interleukin-6 concentration. However, because of the proposed role of interleukin-6 in the regulation of metabolism, it was unclear whether this represented a reduced response to muscle damage or some form of interaction with the metabolic demands of the activity. Therefore, the aim of the present study was to investigate the effect of the same form of supplementation on a bout of exercise that initiated similar muscle damage but had a low metabolic cost. Fourteen male subjects were allocated to either a placebo (P) or a vitamin C (VC) group. The VC group consumed 200 mg of ascorbic acid twice a day for 14 days prior to a bout of exercise and for the 3 days after exercise. The P group consumed identical capsules that contained 200 mg lactose. Subjects performed 30 min of downhill running at a gradient of −18% and recovery was monitored for up to 3 days after exercise. Plasma VC concentrations in the VC group increased following supplementation. Nevertheless, downhill running provoked a similar increase in circulating markers of muscle damage (creatine kinase activity and myoglobin concentration) and muscle soreness in P and VC groups. Similarly, although downhill running increased plasma interleukin-6, there was no effect from VC supplementation. These results suggest that vitamin C supplementation does not affect interleukin-6 concentrations following eccentric exercise that has a low metabolic component.

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References

  • Borg G (1982) Psychophysical bases of perceived exertion. Med Sci Sports Exerc 14:377–381

    CAS  PubMed  Google Scholar 

  • Croisier J, Camus G, Venneman I, Deby-Dupont G, Juchmes-Ferir A, Lamy M, Crielaard J, Deby C, Duchateau J (1999) Effects of training on exercise-induced muscle damage and interleukin 6 production. Muscle Nerve 22:208–212

    CAS  Google Scholar 

  • Duarte J, Appell H, Carvalho F, Bastos M, Soares J (1993) Endothelium-derived oxidative stress may contribute to exercise-induced muscle damage. Int J Sports Med 14:440–443

    CAS  PubMed  Google Scholar 

  • Febbraio MA, Pedersen BK (2002) Muscle-derived interleukin-6: mechanisms for activation and possible biological roles. FASEB J 16:1335–1347

    Article  CAS  PubMed  Google Scholar 

  • Fielding R, Manfredi T, Ding W, Fiatarone M, Evans W, Cannon J (1993) Acute phase response in exercise III. Neutrophil and IL-1β accumulation in skeletal muscle. Am J Physiol 265:R166–R172

    CAS  PubMed  Google Scholar 

  • Grimble R (1996) Interaction between nutrients, pro-inflammatory cytokines and inflammation. Clin Sci 91:121–130

    CAS  PubMed  Google Scholar 

  • Hellsten Y, Frandsen U, Ørthenblad N, Sjødin B, Richter E (1997) Xanthine oxidase in human skeletal muscle following eccentric exercise: a role in inflammation. J Physiol (Lond) 498:239–248

    Google Scholar 

  • Jakeman P, Maxwell S (1993) Effect of antioxidant vitamin supplementation on muscle function after eccentric exercise. Eur J Appl Physiol 67:426–430

    Google Scholar 

  • Lapointe BM, Frenette J, Cote CH (2002) Lengthening contraction-induced inflammation is linked to secondary damage but devoid of neutrophil invasion. J Appl Physiol 92:1995–2004

    Google Scholar 

  • Levine M, Conry-Cantilena C, Wang Y, Welch R, Washko P, Dhariwal K, Park J, Lazarev A, Graumlich J, King J, Cantilena LR (1996) Vitamin C pharmacokinetics in healthy volunteers: Evidence for a recommended dietary allowance. Proc Natl Acad Sci USA 93:3704–3709

    Article  CAS  PubMed  Google Scholar 

  • MacIntyre D, Reid WD, McKenzie DC (1995) Delayed muscle soreness: the inflammatory response to muscle injury and its clinical implications. Sports Med 20:24–40

    CAS  PubMed  Google Scholar 

  • MacIntyre D, Reid W, Lyster D, Szasz I, McKenzie D (1996) Presence of WBC, decreased strength, and delayed soreness in muscle after eccentric exercise. J Appl Physiol 80:1006–1013

    CAS  Google Scholar 

  • Maughan RJ, Donnelly AE, Gleeson M, Whiting PH, Walker KA, Clough PJ (1989) Delayed-onset muscle damage and lipid peroxidation in man after a downhill run. Muscle Nerve 12:332–336

    CAS  PubMed  Google Scholar 

  • Nicholas CW, Tsintzas K, Boobis L, Williams C (1999) Carbohydrate-electrolyte ingestion during intermittent high- intensity running. Med Sci Sports Exerc 31:1280–1286

    Article  CAS  PubMed  Google Scholar 

  • Nicholas CW, Nuttall FE, Williams C (2000) The Loughborough Intermittent Shuttle Test: A field test that simulates the activity pattern of soccer. J Sports Sci 18:97–104

    CAS  PubMed  Google Scholar 

  • Nieman DC, Peters EM, Henson DA, Nevines EI, Thompson MM (2000) Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res 20:1029–1035

    Google Scholar 

  • Petersen EW, Ostrowski K, Ibfelt T, Richelle M, Offord E, Halkjaer-Kristensen J, Pedersen BK (2001) Effect of vitamin supplementation on cytokine response and on muscle damage after strenuous exercise. Am J Physiol 280:C1570–C1575

    CAS  Google Scholar 

  • Schwane J, Johnson S, Vandenakker C, Armstrong R (1983) Delayed-onset muscular soreness and plasma CPK and LDH activities after downhill running. Med Sci Sports Exerc 15:51–56

    CAS  PubMed  Google Scholar 

  • Shephard RJ, Shek PN (1998) Immune responses to inflammation and trauma: a physical training model. Can J Physiol Pharmacol 76:469–472

    Article  CAS  Google Scholar 

  • Smith L (1991) Acute inflammation: the underlying mechanism in delayed onset muscle soreness? Med Sci Sports Exerc 23:542–551

    CAS  PubMed  Google Scholar 

  • Sorichter S, Mair J, Koller A, Gebert W, Rama D, Calzolari C, Artner-Dworzak E, Puschendorf B (1997) Skeletal troponin I as a marker of exercise-induced muscle damage. J Appl Physiol 83:1076–1082

    CAS  PubMed  Google Scholar 

  • Thomas JR, Salazar W, Landers DM (1991) What is missing in p<0.05? Effect Size. Res Q Exerc Sport 62:344–348

    CAS  PubMed  Google Scholar 

  • Thompson D, Nicholas CW, Williams C (1999) Muscle soreness following prolonged intermittent high-intensity shuttle running. J Sports Sci 17:387–395

    Article  CAS  PubMed  Google Scholar 

  • Thompson D, Williams C, Kingsley M, Nicholas CW, Lakomy HKA, McArdle F, Jackson MJ (2001a) Muscle soreness and damage parameters after prolonged intermittent shuttle-running following acute vitamin C supplementation. Int J Sports Med 22:68–75

    Article  CAS  PubMed  Google Scholar 

  • Thompson D, Williams C, McGregor SJ, Nicholas CW, McArdle F, Jackson MJ, Powell JR (2001b) Prolonged vitamin C supplementation and recovery from demanding exercise. Int J Sport Nutr Exerc Metab 11:466–481

    CAS  PubMed  Google Scholar 

  • Thompson D, Williams C, Garcia-Roves P, McGregor S, McArdle F, Jackson MJ (2003) Post-exercise vitamin C supplementation and recovery from demanding exercise. Eur J Appl Physiol 89:393–400

    CAS  PubMed  Google Scholar 

  • Vassilakopoulos T, Karatza M-H, Katsaounou P, Kollintza A, Zakynthinos S, Roussos C (2003) Antioxidants attenuate the plasma cytokine response to exercise in humans. J Appl Physiol 94:1025–1032

    CAS  PubMed  Google Scholar 

  • Weiss S (1989) Tissue destruction by neutrophils. N Engl J Med 320:365–376

    CAS  PubMed  Google Scholar 

  • Williams C, Nute M, Broadbank L, Vinall S (1990) Influence of fluid intake on endurance running performance. Eur J Appl Physiol 60:112–119

    CAS  Google Scholar 

  • Willoughby DS, McFarlin B, Bois C (2003) Interleukin-6 expression after repeated bouts of eccentric exercise. Int J Sports Med 24:15–21

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Sport and Exercise Science, University of Bath, Bath , BA2 7AY, UK

    D. Thompson

  2. Human Muscle Metabolism Research Group, Loughborough University, Loughborough , LE11 3TU, UK

    D. M. Bailey, J. Hill & C. Williams

  3. Colworth Laboratory, Unilever Research, Bedford , MK441LQ, UK

    T. Hurst & J. R. Powell

Authors
  1. D. Thompson
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  2. D. M. Bailey
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  3. J. Hill
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  4. T. Hurst
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  5. J. R. Powell
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  6. C. Williams
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Correspondence to D. Thompson.

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Thompson, D., Bailey, D.M., Hill, J. et al. Prolonged vitamin C supplementation and recovery from eccentric exercise. Eur J Appl Physiol 92, 133–138 (2004). https://doi.org/10.1007/s00421-004-1064-y

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