Abstract
Purpose
To assess the radiation exposure of radon spa personnel in Jáchymov, Czech Republic.
Methods
The frequency of micronucleus-containing cells and the percentage of centromere-free micronuclei (micronuclei containing only acentric fragments) was determined in peripheral blood lymphocytes of 42 individuals working at the Jáchymov spa and 42 control individuals.
Results
There was a highly significant increase in the frequency of micronucleus-containing cells as well as the percentage of centromere-free micronuclei in the lymphocytes of spa personnel versus controls. No individual dosimetry data were available. A comparison with results from currently active uranium miners suggests that the individuals examined at the Jáchymov spa had accumulated effective doses in the order of several tens of mSv, very similar to those of the miners.
Conclusion
The spa personnel in Jáchymov needs to be monitored on an individual level.
Similar content being viewed by others
References
Abend M, Blakely WF, van Beuningen D (1995) Simplified and optimized kinetochore detection: cytogenetic marker for late-G2 cells. Mutat Res 334:39–47
Becker K (2004) One century of radon therapy. Int J Low Radiat 1:334–357
Bochicchio F (2011) The newest international trend about regulation of indoor radon. Radiat Prot Dosimetry 146:2–5
Campos MP, Pecequilo BR, Mazlili BP (2010) 222Rn and 212Pb exposures at a Brazilian spa. Radiat Prot Dosimetry 141:210–214
Falkenbach A, Kovacs J, Franke A, Jörgens K, Ammer K (2005) Radon therapy for the treatment of rheumatic diseases–review and meta-analysis of controlled clinical trials. Rheumatol Int 25:205–210
Fenech M, Morley AA (1985) Measurement of micronuclei in lymphocytes. Mutat Res 147:29–36
Franke A, Reiner L, Resch KL (2007) Long-term benefit of radon spa therapy in the rehabilitation of rheumatoid arthritis: a randomised, double-blinded trial. Rheumatol Int 27:703–713
Johannes C, Dixius A, Pust M, Hentschel R, Buraczewska I, Staaf E, Brehwens K, Haghdoost S, Nievaart S, Czub J, Braziewicz J, Wojcik A (2010) The yield of radiation-induced micronuclei in early and late-arising binucleated cells depends on radiation quality. Mutat Res 701:80–85
Köstinger C. (2006) Beruflicher Strahlenschutz im Gasteiner Heilstollen. In: Kraus HG, (ed) Wirkungsmechanismen niedriger Strahlendosen. Forschung für Radon-Balneotherapie und Strahlenschutz. Schlema, Radiz pp 178–180
Kryscio A, Ulrich Müller WU, Wojcik A, Kotschy N, Grobelny S, Streffer C (2001) A cytogenetic analysis of the long-term effect of uranium mining on peripheral lymphocytes using the micronucleus-centromere assay. Int J Radiat Biol 77:1087–1093
Marušiaková M, Gregor Z, Tomášek L (2011) A review of exposures to radon, long-lived radionuclides and external gamma at the Czech uranium mine. Radiat Prot Dosimetry 145:248–251
Müller WU, Streffer C (1994) Micronucleus assays. In: Obe G (ed) Advances in mutagenesis research. Springer, Berlin, pp 1–134
Olszewski J, Chodak M, Jankowski J (2008) An assay of the current exposure to radon of spa workers in Poland. Med Pr 59:35–38
Rühle H. (2006) Neue Erkenntnisse zur Strahlenexposition von Patienten und Personal durch Radon. In: Kraus HG (ed) Wirkungsmechanismen niedriger Strahlendosen. Forschung für Radon-Balneotherapie und Strahlenschutz. Schlema, Radiz, pp 168–177
Soto J, Gómez J (1999) Occupational doses from radon in Spanish spas. Health Phys 76:398–401
Thomson EJ, Perry PE (1988) The identification of micronucleated chromosomes: a possible assay for aneuploidy. Mutagenesis 3:415–418
Vral A, Thierens H, De Ridder L (1997) In vitro micronucleus-centromere assay to detect radiation-damage induced by low doses in human lymphocytes. Int J Radiat Biol 71:61–68
WHO handbook on indoor radon—a public health perspective, (2009) available on-line at http://www.who.int/ionizing_radiation/env/radon/en/
Zdrojewicz Z, Strzelczyk JJ (2006) Radon treatment controversy. Dose Response 19:106–118
Zeni O, Chiavoni AS, Sannino A, Antolini A, Forigo D, Bersani F, Scarfì MR (2003) Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro to 900 MHz electromagnetic fields. Radiat Res 160:152–158
Zölzer F, Freitinger-Skalická Z, Havránková R, Hon Z, Navrátil L, Rosina J, Škopek J (2011) Enhanced frequency of micronuclei in lymphocytes from current as opposed to former uranium miners. J Appl Biomed 9:151–156
Zölzer F, Hon Z, Freitinger-Skalická Z, Havránková R, Navrátil L, Rosina J, Škopek J (2012) Micronuclei in lymphocytes from currently active uranium miners. Radiat Environ Biophys (in press)
Acknowledgments
We thank Ing. Hana Placakova for reliable technical assistance and Ing. Ladislav Beránek, CSc., MBA for help with the statistics. These investigations have been supported by the Czech Ministry of Education, Youth and Sports within the framework of the National Research Program II (NPV II, project 2B08001).
Conflict of interest
None declared.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zölzer, F., Hon, Z., Skalická, Z.F. et al. Micronuclei in lymphocytes from radon spa personnel in the Czech Republic. Int Arch Occup Environ Health 86, 629–633 (2013). https://doi.org/10.1007/s00420-012-0795-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00420-012-0795-z