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Effect of light on the calretinin and calbindin expression in skin club cells of adult zebrafish

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Abstract

In the last decade, zebrafish has been used as a model for the study of several human skin diseases. The epidermis of Danio rerio is composed of keratinocytes and two types of secretory cells: mucous cells and club cells. Club cells have multiple biological functions and among them may be important in the protection against ultraviolet damage through the proliferative response or through the increased production of protective substances. Calcium-binding proteins such as calbindin D28K and calretinin are used as markers of nervous and enteric nervous systems, but they are present in numerous other cells. These proteins are involved in a wide variety of cell activities, such as cytoskeletal organization, cell motility and differentiation, cell cycle regulation and neuroprotective function. In this study we demonstrated, for the first time, the presence of calretinin and calbindin D28K in skin club cells of Danio rerio exposed to different wavelengths by immunohistochemistry analysis. Exposure to white-blue light and blue light causes the expression and colocalization of calbindin-D28K and calretinin. These proteins were moderately expressed and no colocalization was observed in the club cells of the control fish. In zebrafish exposed to continuous darkness for 10 days, in the club cells the two antibodies did not detect any proteins specifically. These results demonstrate that calbindin and calretinin could be involved in the pathophysiology of skin injury due to exposure to short-wavelength visible light spectrums.

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Author notes

  1. E. R. Lauriano and M. C. Guerrera contributed equally to this paper.

Authors and Affiliations

  1. Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V.le F. Stagno D’Alcontres 31, 98166, Messina, Italy

    E. R. Lauriano & S. Pergolizzi

  2. Department of Veterinary Sciences, Zebrafish Neuromorphology Lab, University of Messina, Polo Universitario Dell’Annunziata, 98168, Messina, Italy

    M. C. Guerrera, R. Laurà, G. Capillo, M. Aragona, F. Abbate & A. Germanà

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  1. E. R. Lauriano
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Contributions

ERL and MCG: carried out the project of the study, the data analysis/interpretation and the drafting of the manuscript. RL and FA: provided guidance and assisted with data analysis/interpretation; SP, GC and MA: participated in data collection, analysis and visualization, statistical analyses and graphical elaborations; also, were responsible for formatting all figures. AG: critical revision of the manuscript and approval of the article.

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Correspondence to M. C. Guerrera.

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Lauriano, E.R., Guerrera, M.C., Laurà, R. et al. Effect of light on the calretinin and calbindin expression in skin club cells of adult zebrafish. Histochem Cell Biol 154, 495–505 (2020). https://doi.org/10.1007/s00418-020-01883-9

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