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Histochemistry and Cell Biology

, Volume 150, Issue 1, pp 77–82 | Cite as

Detection of pro-apoptotic Bax∆2 proteins in the human cerebellum

  • Adriana Mañas
  • Aislinn Davis
  • Sydney Lamerand
  • Jialing Xiang
Short Communication
  • 70 Downloads

Abstract

Bax∆2 is a pro-apoptotic protein originally discovered in colon cancer patients with high microsatellite instability. Unlike most pro-apoptotic Bax family members, Bax∆2 mediates cell death through a non-mitochondrial caspase 8-dependent pathway. In the scope of analyzing the distribution of Bax∆2 expression in human tissues, we examined a panel of human brain samples. Here, we report four cerebellar cases in which the subjects had no neurological disorder or disease documented. We found Bax∆2 positive cells scattered in all areas of the cerebellum, but most strikingly concentrated in Purkinje cell bodies and dendrites. Two out the four subjects tested had strong Bax∆2-positive staining in nearly all Purkinje cells; one was mainly negative; and one had various levels of positive staining within the same sample. Further genetic analysis of the Purkinje cell layer, collected by microdissection from two subjects, showed that the samples contained G7 and G9 Bax microsatellite mutations. Both subjects were young and had no diseases reported at the time of death. As the distribution of Bax∆2 is consistent with that known for Baxα, but in a less ubiquitous manner, these results may imply a potential function of Bax∆2 in Purkinje cells.

Keywords

Bax∆2 Cerebellum Purkinje cells Apoptosis Human brain 

Notes

Acknowledgements

This work was supported by the National Institutes of Health (R15 CA195526).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

418_2018_1669_MOESM1_ESM.pdf (402 kb)
Supplementary material 1 (PDF 402 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Adriana Mañas
    • 1
  • Aislinn Davis
    • 1
  • Sydney Lamerand
    • 1
  • Jialing Xiang
    • 1
  1. 1.Department of BiologyIllinois Institute of TechnologyChicagoUSA

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