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Expression and distribution patterns of spermine, spermidine, and putrescine in rat hair follicle

Histochemistry and Cell Biology volume 149, pages 161–167 (2018)Cite this article

Abstract

No expression and distribution patterns of polyamines (PAs), spermine, spermidine, and their precursor putrescine in mammalian hair follicle are available, although polyamines are known to correlate well with hair growth and epidermal tumor genesis. Immunohistochemistry (IHC) using our original two monoclonal antibodies (mAbs) ASPM-29 specific for spermine or spermidine, and APUT-32 specific for putrescine allowed us to detect immunoreactivity for polyamines in hair follicles from normal adult rats. A wide range of immunoreactivity for the total spermine and spermidine was observed in the compartments of hair follicle: The highest degree of immunoreactivity for polyamines was observed in the matrix, in the Huxley’s layer, in the deeper Henle’s layer, and in the cuticle of the inner root sheath/the hair cuticle, while moderate immunoreactivity existed in the lower-to-mid cortex and the companion layer, followed by lower immunoreactivity in the outer root sheath, including the bulge region and in the deeper medulla, in which the immunoreactivity was also evident in their nuclei. In addition, somewhat surprisingly, with IHC by APUT-32 mAb, we detected significant levels of putrescine in the compartments, in which the immunostaining pattern was the closely similar to that of the total spermine and spermidine. Thus, among these compartments, the cell types of the matrix, the Huxley’s layer, the deeper Henle’s layer, and the cuticle of the inner root sheath/the hair cuticle seem to have the biologically higher potential in compartments of anagen hair follicle, maybe suggesting that they are involved more critically in the biological event of hair growth. In addition, we noted sharp differences of immunostaining by IHCs between ASPM-29 mAb and APUT-32 mAb in the epidermis cells and fibroblast. ASPM-29 mAb resulted in strong staining in both the cell types, but APUT-32 mAb showed only very light staining in both types. Consequently, the use of the two IHCs could be extremely useful in further studies on hair cycle and epidermal tumor genesis experimentally or clinically.

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Abbreviations

PAs:

Polyamine(s)

Spm:

Spermine

Spd:

Spermidine

Put:

Putrescine

t-SP:

Total Spm and Spd

IHC:

Immunohistochemistry

ASPM-29 mAb:

Anti-spermine monoclonal antibody

APUT-32 mAb:

Anti-putrescine monoclonal antibody

RT:

Room temperature

HF:

Hair follicle

PBS:

Phosphate-buffered saline

TBS:

50 mM Tris–HCl buffer pH 7.4 containing 0.86% NaCl

TBST:

TBS supplemented with 0.1% Triton X-100

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Authors and Affiliations

  1. Department of Applied Life Science, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, Kumamoto, 860-0082, Japan

    Yutaro Yamamoto, Kunio Fujiwara & Masashi Shin

  2. Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1Honjyo, Kumamoto, 860-8556, Japan

    Takamitsu Makino, Hideo Kudo & Hironobu Ihn

  3. Department of Molecular Biology, The Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo, 105-8461, Japan

    Yasuko Murakami & Senya Matsufuji

Authors
  1. Yutaro Yamamoto
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  2. Takamitsu Makino
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  3. Hideo Kudo
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  4. Hironobu Ihn
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  5. Yasuko Murakami
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  6. Senya Matsufuji
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  7. Kunio Fujiwara
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  8. Masashi Shin
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Correspondence to Kunio Fujiwara.

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Yamamoto, Y., Makino, T., Kudo, H. et al. Expression and distribution patterns of spermine, spermidine, and putrescine in rat hair follicle. Histochem Cell Biol 149, 161–167 (2018). https://doi.org/10.1007/s00418-017-1621-1

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  • DOI: https://doi.org/10.1007/s00418-017-1621-1

Keywords

  • Polyamine(s)
  • Spermine
  • Spermidine
  • Putrescine
  • Immunohistochemistry
  • Hair follicle(s)
  • Monoclonal antibody
  • Rat