Histochemistry and Cell Biology

, Volume 148, Issue 5, pp 569–573 | Cite as

Quality control in diagnostic immunohistochemistry: integrated on-slide positive controls

  • A. Bragoni
  • A. Gambella
  • S. Pigozzi
  • M. Grigolini
  • R. Fiocca
  • L. Mastracci
  • F. Grillo
Short Communication


Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy—PB—tool) procedure to construct positive controls which can be integrated in the patient’s tissue slide. Sixteen frequently used control blocks were selected and multiple cylindrical samples were obtained using a 5-mm diameter punch biopsy tool, separately re-embedding them in single blocks. For each diagnostic immunoreaction requiring a positive control, an integrated PB-control section (cut from the appropriate PB-control block) was added to the top right corner of the diagnostic slide before immunostaining. This integrated control technique permitted a saving of 4.75% in total direct lab costs and proved to be technically feasible and reliable. Our proposal is easy to perform and within the reach of all pathology labs, requires easily available tools, its application costs is less than using external paired controls and ensures that a specific control for each slide is always available.


Quality assurance Immunohistochemistry Positive controls Cost-saving 



We wish to thank all the immunohistochemistry laboratory staff who helped with the project: Viviana Accorrà, Laura Barabino, Silvia Bonadio, Barbara Garzoglio and Laura Zito.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Histopathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC)University of GenoaGenoaItaly
  2. 2.Ospedale Policlinico San MartinoGenoaItaly

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