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Phosphorylation of histone H3 on Ser-10 by Aurora B is essential for chromosome condensation in porcine embryos during the first mitotic division

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Abstract

Phosphorylation of histone H3 on Ser-10 (H3S10ph) is involved in regulating mitotic chromosome condensation and decondensation, which plays an important regulatory role during mitotic cell cycle progression in mammalian cells. However, whether H3S10ph plays a similar role in early porcine embryos during the first mitotic division remains uncertain. In this study, the subcellular localization and possible roles of H3S10ph were evaluated in the first mitotic cell cycle progression of porcine embryos using western blot, indirect immunofluorescence and barasertib (H3S10ph upstream regulator Aurora-B inhibitor) treatments. H3S10ph exhibited a dynamic localization pattern and was localized to chromosomes from prometaphase to anaphase stages. Treatment of porcine embryos with barasertib inhibited mitotic division at the prophase stage and was associated with a defect in chromosome condensation accompanied by the reduction of H3S10ph. These results indicated that H3S10ph is involved in the first mitotic division in porcine embryos through its regulatory function in chromosome condensation, which further affects porcine embryo cell cycle progression during mitotic division.

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Acknowledgements

The work was supported by the National Natural Science Foundation of China (31572589), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20130097110020), and the Priority Academic Program Development (PAPD) of Jiangsu Province. We thank Hao Zhang for providing porcine ovaries. We express our gratitude to Guoqing Huang for his help with using confocal laser-scanning microscopy.

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Authors and Affiliations

  1. College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, 210095, China

    Changchao Chen, Zixiao Zhang, Panpan Cui, Yaya Liao, Yue Zhang, Lingyun Yao, Rong Rui & Shiqiang Ju

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  1. Changchao Chen
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  2. Zixiao Zhang
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  3. Panpan Cui
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  4. Yaya Liao
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  5. Yue Zhang
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  8. Shiqiang Ju
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Correspondence to Shiqiang Ju.

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The authors declare no conflict of interest.

Ethical approval

The animals used in this study and their care were according to the guidelines of Animal Research Institute Committee which is prescribed by Nanjing Agricultural University, China. The workers who executed the slaughtering complied with the pig slaughtering regulations (State Council of the People’s Republic of China, No. 666).

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Chen, C., Zhang, Z., Cui, P. et al. Phosphorylation of histone H3 on Ser-10 by Aurora B is essential for chromosome condensation in porcine embryos during the first mitotic division. Histochem Cell Biol 148, 73–83 (2017). https://doi.org/10.1007/s00418-017-1546-8

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