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Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells

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Abstract

High fructose intake is known to be associated with increased plasma triglyceride concentration, impaired glucose tolerance, insulin resistance, and high blood pressure. In addition, excess fructose intake is also thought to be a risk factor for dementia. Previous immunohistochemical studies have shown the presence of glucose transporter 5 (GLUT5), a major transporter of fructose, in the epithelial cells of the choroid plexus and ependymal cells in the brains of humans, rats, and mice, while GLUT2, a minor transporter of fructose, was localized in the ependymal cells of rat brain. In this study, immunoreactivity for the fructose transporter GLUT8 was observed in the cytoplasm of the epithelial cells in the choroid plexus and in the ependymal cells of the brains of humans and mice. These structures were not immunoreactive for GLUT7, GLUT11, and GLUT12. Our findings support the hypothesis of the transport of intravascular fructose through the epithelial cells of the choroid plexus and the ependymal cells.

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Acknowledgments

This study was supported by a Grant-in-Aid for Scientific Research (C) 26430055 (M.U.) for the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The authors thank Ms. K. Yasutomi for editorial assistance.

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Authors and Affiliations

  1. Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho (Kita-gun), Kagawa, 761-0793, Japan

    Ryuta Murakami, Yoichi Chiba, Koichi Matsumoto, Machi Kawauchi, Ryuji Fujihara, Masato Mashima & Masaki Ueno

  2. Department of Biochemistry, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan

    Kazuhito Tsuboi

  3. Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan

    Ryuji Fujihara & Tetsuji Yamamoto

  4. Department of Perinatology and Gynecology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, 761-0793, Japan

    Masato Mashima & Kenji Kanenishi

Authors
  1. Ryuta Murakami
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  2. Yoichi Chiba
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  3. Kazuhito Tsuboi
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  10. Masaki Ueno
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Correspondence to Masaki Ueno.

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Murakami, R., Chiba, Y., Tsuboi, K. et al. Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells. Histochem Cell Biol 146, 231–236 (2016). https://doi.org/10.1007/s00418-016-1444-5

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  • DOI: https://doi.org/10.1007/s00418-016-1444-5

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