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Histochemistry and Cell Biology

, Volume 142, Issue 1, pp 19–41 | Cite as

Phototransformable fluorescent proteins: which one for which application?

  • Virgile AdamEmail author
Review

Abstract

In these last two decades , fluorescent proteins (FPs) have become highly valued imaging tools for cell biology, owing to their compatibility with living samples, their low levels of invasiveness and the possibility to specifically fuse them to a variety of proteins of interest. Remarkably, the recent development of phototransformable fluorescent proteins (PTFPs) has made it possible to conceive optical imaging experiments that were unimaginable only a few years ago. For example, it is nowadays possible to monitor intra- or intercellular trafficking, to optically individualize single cells in tissues or to observe single molecules in live cells. The tagging specificity brought by these genetically encoded highlighters leads to constant progress in the engineering of increasingly powerful, versatile and non-cytotoxic FPs. This review is focused on the recent developments of PTFPs and highlights their contribution to studies within cells, tissues and even living organisms. The aspects of single-molecule localization microscopy, intracellular tracking of photoactivated molecules, applications of PTFPs in biotechnology/optobiology and complementarities between PTFPs and other microscopy techniques are particularly discussed.

Keywords

Fluorescent proteins Photoactivation Live cell Imaging Super-resolution Biotechnology 

Notes

Acknowledgments

VA is grateful to Dominique Bourgeois for stimulating and fruitful discussions and to Jacques-Philippe Colletier for critical reading of the manuscript. Institutional Grants from the CNRS, CEA and UJF and financial support by the ANR (ANR-2011-BSV5-012-01 NOBLEACH) are acknowledged.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institut de Biologie Structurale (IBS)Univ. Grenoble AlpesGrenobleFrance
  2. 2.IBSCNRSGrenobleFrance
  3. 3.CEA, DSVIBSGrenobleFrance

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