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Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells

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Abstract

Dental pulp stem cells (hDP-SCs) were primarily derived from pulp tissues of primary incisors, exfoliated deciduous and permanent third molar teeth. To understand the characteristics of hDP-SCs from impacted third molar, proliferation capacities, gene expression profiles, phenotypic, ultrastructural, and differentiation characteristics were analyzed in comparison with human bone marrow-derived mesenchymal stem cells (hBM-MSCs), extensively. hDP-SCs showed more developed and metabolically active cells. Contrary to hBM-MSCs, hDP-SCs strongly expressed both cytokeratin (CK)-18 and -19, which could involve in odontoblast differentiation and dentine repair. The intrinsic neuro-glia characteristics of hDP-MSCs were demonstrated by the expression of several specific transcripts and proteins of neural stem cell and neurons. These cells not only differentiate into adipogenic, osteogenic, and chondrogenic lineage, but also share some special characteristics of expressing some neural stem cell and epithelial markers. Under defined conditions, hDP-SCs are able to differentiate into both neural and vascular endothelial cells in vitro. Dental pulp might provide an alternative source for human MSCs. hDP-SCs with a promising differentiation capacity could be easily isolated, and possible clinical use could be developed for neurodegenerative and oral diseases in the future.

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Acknowledgment

This study was supported by grants [108S404] and [108S291] of the Scientific and Research Council of Turkey (TUBITAK). We thank Alpaslan Okcu and Cansu Subası for their excellent technical assistance.

Conflict of interest

The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Kocaeli University, 41380, Kocaeli, Turkey

    Erdal Karaöz, Pınar Cetinalp Demircan, Özlem Sağlam, Ayca Aksoy & Gökhan Duruksu

  2. Faculty of Medicine, Department of Histology and Embryology, Hacettepe University, 06100, Ankara, Turkey

    Figen Kaymaz

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  1. Erdal Karaöz
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  2. Pınar Cetinalp Demircan
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  3. Özlem Sağlam
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  5. Figen Kaymaz
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  6. Gökhan Duruksu
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Correspondence to Erdal Karaöz.

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Karaöz, E., Demircan, P.C., Sağlam, Ö. et al. Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells. Histochem Cell Biol 136, 455–473 (2011). https://doi.org/10.1007/s00418-011-0858-3

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  • DOI: https://doi.org/10.1007/s00418-011-0858-3

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