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Pnn and SR family proteins are differentially expressed in mouse central nervous system

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Abstract

Pinin (pnn) is an SR-related protein that is ubiquitously expressed in most cell types and functions in regulating pre-mRNA splicing and mRNA export. Previously, we demonstrated that pnn is expressed in all tissues during mouse embryonic development with highest levels of expression in the central nervous system (CNS). Here we show that pnn and other SR proteins including SC35 are differentially expressed in the adult mouse CNS, displaying cell type-specific distribution patterns. Immunohistochemical analysis of whole-brain sections showed that levels of pnn and SR proteins expression were very low or nonexistent in the corpus callosum and white matter of cerebellum and spinal cord. Double-immunostaining with antibodies specific to neuron or glial cells showed that most astrocytes and microglia expressed neither pnn nor SR proteins. In contrast, oligodendrocytes and neurons expressed moderate and high levels, respectively, of both pnn and SR proteins. These results suggest that astrocytes are unique among cell types of neuroblast origin in terms of expression SR family proteins. Our results pave the way for future studies of the functional roles of pnn and SR family proteins in adults.

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Acknowledgments

This work was supported by grants from Chang Gung Memorial Hospital (CMRPD 170073), the Ministry of Education (EMRPD 190481) and the National Science Council, ROC (NSC-98-2320-B-182-019-MY3).

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Authors and Affiliations

  1. Graduate Institute of Biomedical Sciences, Epithelial Biology Laboratory, Department of Anatomy, Chang Gung University Medical College, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan, Republic of China

    Shu-Yuan Hsu

  2. Transgenic Mouse Core-Lab, Epithelial Biology Laboratory, Department of Anatomy, Chang Gung University Medical College, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan, Republic of China

    Yen-Jung Chen

  3. Graduate Institute of Biomedical Sciences, Transgenic Mouse Core-Lab, Epithelial Biology Laboratory, Department of Anatomy, Chang Gung University Medical College, 259 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, 333, Taiwan, Republic of China

    Pin Ouyang

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  1. Shu-Yuan Hsu
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  2. Yen-Jung Chen
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Correspondence to Pin Ouyang.

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Hsu, SY., Chen, YJ. & Ouyang, P. Pnn and SR family proteins are differentially expressed in mouse central nervous system. Histochem Cell Biol 135, 361–373 (2011). https://doi.org/10.1007/s00418-011-0795-1

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