Histochemistry and Cell Biology

, Volume 134, Issue 5, pp 445–452 | Cite as

Identification of FABP7 in fibroblastic reticular cells of mouse lymph nodes

  • Nobuko Tokuda
  • Toshiaki Adachi
  • Yasuhiro Adachi
  • Mayumi Higashi
  • Kazem Sharifi
  • Tuerhong Tuerxun
  • Tomoo Sawada
  • Hisatake Kondo
  • Yuji Owada
Original Paper


Fatty acids and their metabolites regulate immune cell function. The present study was undertaken to examine the detailed distribution of fatty acid binding proteins (FABPs), the cytosolic chaperones of fatty acids, in mouse peripheral immune organs. Using immunohistochemistry, FABP7 was localized to the alpha-smooth muscle actin (SMA)+ fibroblastic reticular cells, which construct the stromal reticula in the T cell areas of the peripheral lymph nodes and spleen. Immunoelectron microscopy showed that FABP7+ cells enclosed the collagen fibers, forming a conduit system, which transport lymph and associated low-molecular-mass proteins. In contrast, FABP5+ cells were distributed throughout the lymph node and contained well-developed lysosome and phagocytic materials within the cytoplasm. The mesenteric lymph nodes of FABP7 knockout mice showed normal histological features, but the percentage of CD4+ cells was significantly increased compared with that in wild-type mice. These data indicate that FABP7 may be involved in T cell homeostasis, possibly by modulating lipid metabolism in fibroblastic reticular cells within the peripheral lymph nodes.


Fibroblastic reticular cell Fatty acid binding protein Lymph node Spleen 

Supplementary material

418_2010_754_MOESM1_ESM.doc (48 kb)
Supplementary Table 1 (DOC 48 kb)
418_2010_754_MOESM2_ESM.tif (50 kb)
Supplementary Fig. 1 Gene expression of FABP7 in the mouse mesenteric lymph node (MLN). By RT-PCR analysis, the gene expression of FABP7 was detected clearly in MLN. The expression in the neonatal cerebrum and cerebellum were shown as positive controls (TIFF 49 kb)
418_2010_754_MOESM3_ESM.tif (624 kb)
Supplementary Fig. 2 Localization of FABP7 and CD31 in the paracortex of mouse mesenteric lymph node. Note that CD31 immunoreactivities were observed in the endothelial cells of high endothelial venules (a) and capillary vessels (b), both of which were FABP7 negative. Scale Bar, 20 μm (TIFF 623 kb)
418_2010_754_MOESM4_ESM.tif (134 kb)
Supplementary Fig. 3 Flow cytometry analysis of CD4 and CD8 expression in mesenteric lymph node and spleen cells from WT and FABP7 KO mice. The numbers in each quadrant indicate the percentage of cells. Data are representative of six to nine independent experiments (TIFF 134 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Nobuko Tokuda
    • 1
  • Toshiaki Adachi
    • 1
  • Yasuhiro Adachi
    • 1
  • Mayumi Higashi
    • 1
  • Kazem Sharifi
    • 1
  • Tuerhong Tuerxun
    • 1
  • Tomoo Sawada
    • 1
  • Hisatake Kondo
    • 2
  • Yuji Owada
    • 1
  1. 1.Department of Organ AnatomyYamaguchi University Graduate School of MedicineUbeJapan
  2. 2.Division of Histology, Department of Rehabilitation, Faculty of Health Science and WelfareTohoku Bunka Gakuen UniversitySendaiJapan

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