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Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner

Histochemistry and Cell Biology volume 134, pages 53–57 (2010)Cite this article

Abstract

Manserin is a recently characterized 40-amino acid neuropeptide derived from secretogranin II, a protein belonging to the chromogranin family. Although the physiological roles of manserin have not been elucidated to date, manserin has been shown to distribute in not only the brain but also the endocrine system such as the pituitary and adrenal glands, suggesting its role in the endocrine system. The present study aimed to explore the occurrence and distribution of manserin in the rat pancreas using an immunohistochemical technique with a polyclonal antibody against rat manserin. Immunoreactivity for manserin was readily detected in almost whole islets of Langerhans whereas not at all in the exocrine pancreas. Manserin-expressing cells were not colocalized with the glucagon-secreting cells (α cells), whereas they colocalized with insulin-secreting cells (β cells) and somatostatin-secreting cells (δ cells), although their intracellular distribution was different. These results indicate that manserin, occurring in the endocrine pancreas, may have a potential role in the endocrine system.

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Affiliations

  1. Department of Developmental and Regenerative Medicine, Graduate School of Medicine, Mie University, 2-174, Edobashi, Tsu, Mie, Japan

    Kaori Tano, Akiko Oyabu, Yasura Tashiro, Naoki Kamada & Masaaki Narita

  2. Department of Acupuncture and Moxibustion, Suzuka University of Medical Science, Suzuka, Mie, Japan

    Kaori Tano & Fumio Nasu

  3. Department of Education, Bunkyo University, Tokyo, Japan

    Naoko Narita

Authors
  1. Kaori Tano
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  2. Akiko Oyabu
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  3. Yasura Tashiro
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  4. Naoki Kamada
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  5. Naoko Narita
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  6. Fumio Nasu
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  7. Masaaki Narita
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Correspondence to Masaaki Narita.

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Tano, K., Oyabu, A., Tashiro, Y. et al. Manserin, a secretogranin II-derived peptide, distributes in the rat endocrine pancreas colocalized with islet-cell specific manner. Histochem Cell Biol 134, 53–57 (2010). https://doi.org/10.1007/s00418-010-0709-7

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  • DOI: https://doi.org/10.1007/s00418-010-0709-7

Keywords

  • Neuropeptide
  • Manserin
  • Secretogranin
  • Pancreas