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Characterization of bovine fetal Leydig cells by KIT expression

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Abstract

The origin of fetal Leydig cells (FLC) and whether they share a common lineage with adult Leydig cells (ALC) is still under debate, and a marker to reliably track and isolate fetal Leydig precursor cells remains to be identified. We analyzed KIT positive (KIT+) cells in gonads from bovine fetuses with crown-rump-length (CRL) 2.5–85 cm by immunohistochemistry, and found that KIT expression was gender-specific. In female gonads, expression was mainly associated with epithelial cell cords, which extended from the surface epithelium towards the KIT-negative inner stroma. In male gonads of fetuses, after CRL 2.9 cm, KIT expression was strikingly strong in interstitial cells (IC). Only a few KIT+ cells were detected in the epithelial cell cords and in the stromal layer under the surface epithelium after CRL 3.5 cm. In the male fetuses, KIT expression in IC was a continuous and characteristic feature until full term. At all developmental stages KIT+ areas alternated with anti-Müllerian hormone-positive areas. Platelet-derived growth factor receptor α production was initiated after the expression of KIT at CRL 4.5 cm. Detection of cytochrome P450 side chain cleavage enzyme and steroidogenic acute regulatory protein in KIT+ IC identified them as FLC. KIT+ cells, isolated from testes by magnetic-activated cell sorting, retained their steroidogenic capacity in vitro. Together, these findings show that KIT+ IC of fetal testis correspond to FLC, which can be successfully cultivated for advanced studies.

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Acknowledgments

We cordially thank Prof. J. Y. Picard at the Unité de Recherches sur l’Endocrinologie du Développement, INSERM u493, Clamart, France, for the generous gift of the antibody. We are grateful to Annemarie Brachmann and Angela Ehrlich for their outstanding laboratory support. Katja Sygnecka, Katja Hummitzsch and Daniela Koch provided technical advice during the entire project. We thank Dr. G. Domel and his veterinarian team for their support at Südost Fleisch GmBH, Alternburg Germany. We also acknowledge the assistance of San Francisco Edit in editing this manuscript. Financial support was obtained from the German Research Foundation (DFG-Sp 232/12-1).

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  1. Institute of Anatomy, University of Leipzig, Liebigstrasse 13, 04103, Leipzig, Germany

    Nikoloz Tsikolia, Claudia Merkwitz, Kristina Sass, Katharina Spanel-Borowski & Albert Markus Ricken

  2. Molecular Biology and Immunology Research Division, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-0856, Japan

    Michiharu Sakurai

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  1. Nikoloz Tsikolia
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Correspondence to Albert Markus Ricken.

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K. Spanel-Borowski and A. M. Ricken contributed equally to this work as senior authors.

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Tsikolia, N., Merkwitz, C., Sass, K. et al. Characterization of bovine fetal Leydig cells by KIT expression. Histochem Cell Biol 132, 623–632 (2009). https://doi.org/10.1007/s00418-009-0640-y

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